Development of detached root and leaf assays to evaluate the antagonistic properties of biocontrol agents against Fusarium wilt of banana
The failure of bio-control agents (BCAs) in suppressing Fusarium oxysporum f. sp. cubense tropical race 4 (Foc-TR4) was reported in vivo although antagonism was obtained from in vitro dual culture assay. This study aims to develop rapid assays to validate the antagonism of BCAs using detached banana...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor and Francis
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/88431/1/ABSTRACT.pdf |
| _version_ | 1825936517174919168 |
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| author | Wong, Clement Kiing Fook Chui, Yao Teh Vadamalai, Ganesan Saidi, Noor Baity Zulperi, Dzarifah |
| author_facet | Wong, Clement Kiing Fook Chui, Yao Teh Vadamalai, Ganesan Saidi, Noor Baity Zulperi, Dzarifah |
| author_sort | Wong, Clement Kiing Fook |
| collection | UPM |
| description | The failure of bio-control agents (BCAs) in suppressing Fusarium oxysporum f. sp. cubense tropical race 4 (Foc-TR4) was reported in vivo although antagonism was obtained from in vitro dual culture assay. This study aims to develop rapid assays to validate the antagonism of BCAs using detached banana leaves and roots. Both Trichoderma harzianum and Pseudomonas aeruginosa reduced root lesion length (2.7–3.1 cm) compared to 4.9 cm in TR4 treatment. BCA-treated leaves reduced wilting severity from 93.75 to 33.75%. Trypan blue staining showed lower cell death area in BCA-treated roots and leaves. Reduced leaf wilting was indicated by lower red ink content and higher chlorophyll content in BCA-treated leaves. Both defense-related genes, PR-1 and PR-3 were expressed in BCA-treated roots and leaves which could be associated with reduced root necrosis and leave wilting. In conclusion, both assays could be served as rapid methods to select potential BCAs before attempting large-scale trials. |
| first_indexed | 2024-03-06T10:45:29Z |
| format | Article |
| id | upm.eprints-88431 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:45:29Z |
| publishDate | 2020 |
| publisher | Taylor and Francis |
| record_format | dspace |
| spelling | upm.eprints-884312021-12-28T06:49:36Z http://psasir.upm.edu.my/id/eprint/88431/ Development of detached root and leaf assays to evaluate the antagonistic properties of biocontrol agents against Fusarium wilt of banana Wong, Clement Kiing Fook Chui, Yao Teh Vadamalai, Ganesan Saidi, Noor Baity Zulperi, Dzarifah The failure of bio-control agents (BCAs) in suppressing Fusarium oxysporum f. sp. cubense tropical race 4 (Foc-TR4) was reported in vivo although antagonism was obtained from in vitro dual culture assay. This study aims to develop rapid assays to validate the antagonism of BCAs using detached banana leaves and roots. Both Trichoderma harzianum and Pseudomonas aeruginosa reduced root lesion length (2.7–3.1 cm) compared to 4.9 cm in TR4 treatment. BCA-treated leaves reduced wilting severity from 93.75 to 33.75%. Trypan blue staining showed lower cell death area in BCA-treated roots and leaves. Reduced leaf wilting was indicated by lower red ink content and higher chlorophyll content in BCA-treated leaves. Both defense-related genes, PR-1 and PR-3 were expressed in BCA-treated roots and leaves which could be associated with reduced root necrosis and leave wilting. In conclusion, both assays could be served as rapid methods to select potential BCAs before attempting large-scale trials. Taylor and Francis 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88431/1/ABSTRACT.pdf Wong, Clement Kiing Fook and Chui, Yao Teh and Vadamalai, Ganesan and Saidi, Noor Baity and Zulperi, Dzarifah (2020) Development of detached root and leaf assays to evaluate the antagonistic properties of biocontrol agents against Fusarium wilt of banana. Archives of Phytopathology and Plant Protection, 53 (9-10). 479 - 494. ISSN 0323-5408; ESSN: 1477-2906 https://www.tandfonline.com/doi/abs/10.1080/03235408.2020.1761222 10.1080/03235408.2020.1761222 |
| spellingShingle | Wong, Clement Kiing Fook Chui, Yao Teh Vadamalai, Ganesan Saidi, Noor Baity Zulperi, Dzarifah Development of detached root and leaf assays to evaluate the antagonistic properties of biocontrol agents against Fusarium wilt of banana |
| title | Development of detached root and leaf assays to evaluate the antagonistic properties of biocontrol agents against Fusarium wilt of banana |
| title_full | Development of detached root and leaf assays to evaluate the antagonistic properties of biocontrol agents against Fusarium wilt of banana |
| title_fullStr | Development of detached root and leaf assays to evaluate the antagonistic properties of biocontrol agents against Fusarium wilt of banana |
| title_full_unstemmed | Development of detached root and leaf assays to evaluate the antagonistic properties of biocontrol agents against Fusarium wilt of banana |
| title_short | Development of detached root and leaf assays to evaluate the antagonistic properties of biocontrol agents against Fusarium wilt of banana |
| title_sort | development of detached root and leaf assays to evaluate the antagonistic properties of biocontrol agents against fusarium wilt of banana |
| url | http://psasir.upm.edu.my/id/eprint/88431/1/ABSTRACT.pdf |
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