DNA barcoding analysis of larval fishes in Peninsular Malaysia
Aim: To identify fish larvae to species level by DNA barcoding method using the mitochondrial Cytochrome c Oxidase I (COI) gene. The lack of species identification work on fish larvae in Peninsular Malaysia has warrant this study to be conducted. Methodology: Identification of fish larvae species fr...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Academy of Environmental Biology
2020
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/88497/1/ABSTRACT.pdf |
| _version_ | 1796981981591896064 |
|---|---|
| author | Azmir, Izzati Adilah Esa, Yuzine S. M., Nurul Amin Md Yasin, Ina Salwany |
| author_facet | Azmir, Izzati Adilah Esa, Yuzine S. M., Nurul Amin Md Yasin, Ina Salwany |
| author_sort | Azmir, Izzati Adilah |
| collection | UPM |
| description | Aim: To identify fish larvae to species level by DNA barcoding method using the mitochondrial Cytochrome c Oxidase I (COI) gene. The lack of species identification work on fish larvae in Peninsular Malaysia has warrant this study to be conducted. Methodology: Identification of fish larvae species from mangrove areas of Pendas Johor, Matang Perak, Pekan Pahang and Setiu Terengganu was conducted. Samples were collected from April 2015 to September 2015 using a bongo net, towed at a depth of about 0.5 m from the surface for 5 min against the tidal flow. From the total of 354 fish larvae collected, a representative of 177 fish larvae was selected and sequenced using COI gene Results: Results from BLAST and BOLDSYSTEM search showed all sequences had high percentage identity index and similarity (90% to 100%). Fish larvae were identified through phylogenetics analysis showing monophyletic status between query sequences with reference sequences obtained from own collection and GenBank. The Sillago vittata and Sillago sihama sequences was found to be in separate clusters despite their similar genus. A few strong match of specimens from different genus was found with high bootstrap value (n>90%) through Neighbour-Joining (NJ) and Maximum likelihood (ML) analysis e.g the Paramugil parmatus with Liza melinoptera (NJ =100%, ML = 99%) and Pseudogobius oligactis with Eugnathogobius oligactis (NJ = 92%, ML 94%). Interpretation: Identification of fish larvae were best conducted with the aid of molecular method, DNA barcoding in particular rather than comparative taxonomical studiesalone that able to identify fish larvae sample to genus level at best. |
| first_indexed | 2024-03-06T10:45:38Z |
| format | Article |
| id | upm.eprints-88497 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:45:38Z |
| publishDate | 2020 |
| publisher | Academy of Environmental Biology |
| record_format | dspace |
| spelling | upm.eprints-884972021-12-27T02:44:22Z http://psasir.upm.edu.my/id/eprint/88497/ DNA barcoding analysis of larval fishes in Peninsular Malaysia Azmir, Izzati Adilah Esa, Yuzine S. M., Nurul Amin Md Yasin, Ina Salwany Aim: To identify fish larvae to species level by DNA barcoding method using the mitochondrial Cytochrome c Oxidase I (COI) gene. The lack of species identification work on fish larvae in Peninsular Malaysia has warrant this study to be conducted. Methodology: Identification of fish larvae species from mangrove areas of Pendas Johor, Matang Perak, Pekan Pahang and Setiu Terengganu was conducted. Samples were collected from April 2015 to September 2015 using a bongo net, towed at a depth of about 0.5 m from the surface for 5 min against the tidal flow. From the total of 354 fish larvae collected, a representative of 177 fish larvae was selected and sequenced using COI gene Results: Results from BLAST and BOLDSYSTEM search showed all sequences had high percentage identity index and similarity (90% to 100%). Fish larvae were identified through phylogenetics analysis showing monophyletic status between query sequences with reference sequences obtained from own collection and GenBank. The Sillago vittata and Sillago sihama sequences was found to be in separate clusters despite their similar genus. A few strong match of specimens from different genus was found with high bootstrap value (n>90%) through Neighbour-Joining (NJ) and Maximum likelihood (ML) analysis e.g the Paramugil parmatus with Liza melinoptera (NJ =100%, ML = 99%) and Pseudogobius oligactis with Eugnathogobius oligactis (NJ = 92%, ML 94%). Interpretation: Identification of fish larvae were best conducted with the aid of molecular method, DNA barcoding in particular rather than comparative taxonomical studiesalone that able to identify fish larvae sample to genus level at best. Academy of Environmental Biology 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88497/1/ABSTRACT.pdf Azmir, Izzati Adilah and Esa, Yuzine and S. M., Nurul Amin and Md Yasin, Ina Salwany (2020) DNA barcoding analysis of larval fishes in Peninsular Malaysia. Journal of Environmental Biology, 41 (5). pp. 1295-1308. ISSN 0254-8704; ESSN: 2394-0379 http://www.jeb.co.in/index.php?page=abstract&issue=202009_sep20_spl&number=24 10.22438/jeb/41/5(SI)/MS_23 |
| spellingShingle | Azmir, Izzati Adilah Esa, Yuzine S. M., Nurul Amin Md Yasin, Ina Salwany DNA barcoding analysis of larval fishes in Peninsular Malaysia |
| title | DNA barcoding analysis of larval fishes in Peninsular Malaysia |
| title_full | DNA barcoding analysis of larval fishes in Peninsular Malaysia |
| title_fullStr | DNA barcoding analysis of larval fishes in Peninsular Malaysia |
| title_full_unstemmed | DNA barcoding analysis of larval fishes in Peninsular Malaysia |
| title_short | DNA barcoding analysis of larval fishes in Peninsular Malaysia |
| title_sort | dna barcoding analysis of larval fishes in peninsular malaysia |
| url | http://psasir.upm.edu.my/id/eprint/88497/1/ABSTRACT.pdf |
| work_keys_str_mv | AT azmirizzatiadilah dnabarcodinganalysisoflarvalfishesinpeninsularmalaysia AT esayuzine dnabarcodinganalysisoflarvalfishesinpeninsularmalaysia AT smnurulamin dnabarcodinganalysisoflarvalfishesinpeninsularmalaysia AT mdyasininasalwany dnabarcodinganalysisoflarvalfishesinpeninsularmalaysia |