DNA electrochemical biosensor based on iron oxide/nanocellulose crystalline composite modified screen-printed carbon electrode for detection of Mycobacterium tuberculosis
Death from tuberculosis has resulted in an increased need for early detection to prevent a tuberculosis (TB) epidemic, especially in closed and crowded populations. Herein, a sensitive electrochemical DNA biosensor based on functionalized iron oxide with mercaptopropionic acid (MPA-Fe3O4) nanopartic...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Multidisciplinary Digital Publishing Institute
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/88498/1/ABSTRACT.pdf |
| _version_ | 1796981981802659840 |
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| author | Mat Zaid, Mohd Hazani Che-Engku-Chik, Che Engku Noramalina Yusof, Nor Azah Abdullah, Jaafar Othman, Siti Sarah Issa, Rahizan Md Noh, Mohd Fairulnizal Wasoh, Helmi |
| author_facet | Mat Zaid, Mohd Hazani Che-Engku-Chik, Che Engku Noramalina Yusof, Nor Azah Abdullah, Jaafar Othman, Siti Sarah Issa, Rahizan Md Noh, Mohd Fairulnizal Wasoh, Helmi |
| author_sort | Mat Zaid, Mohd Hazani |
| collection | UPM |
| description | Death from tuberculosis has resulted in an increased need for early detection to prevent a tuberculosis (TB) epidemic, especially in closed and crowded populations. Herein, a sensitive electrochemical DNA biosensor based on functionalized iron oxide with mercaptopropionic acid (MPA-Fe3O4) nanoparticle and nanocellulose crystalline functionalized cetyl trimethyl ammonium bromide (NCC/CTAB) has been fabricated for the detection of Mycobacterium tuberculosis (MTB). In this study, a simple drop cast method was applied to deposit solution of MPA-Fe3O4/NCC/CTAB onto the surface of the screen-printed carbon electrode (SPCE). Then, a specific sequence of MTB DNA probe was immobilized onto a modified SPCE surface by using the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling mechanism. For better signal amplification and electrochemical response, ruthenium bipyridyl Ru(bpy)32+ was assigned as labels of hybridization followed by the characteristic test using differential pulse voltammetry (DPV). The results of this biosensor enable the detection of target DNA until a concentration as low as 7.96 × 10−13 M with a wide detection range from 1.0 × 10−6 to 1.0 × 10−12 M. In addition, the developed biosensor has shown a differentiation between positive and negative MTB samples in real sampel analysis. |
| first_indexed | 2024-03-06T10:45:38Z |
| format | Article |
| id | upm.eprints-88498 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:45:38Z |
| publishDate | 2020 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | upm.eprints-884982021-12-27T03:08:15Z http://psasir.upm.edu.my/id/eprint/88498/ DNA electrochemical biosensor based on iron oxide/nanocellulose crystalline composite modified screen-printed carbon electrode for detection of Mycobacterium tuberculosis Mat Zaid, Mohd Hazani Che-Engku-Chik, Che Engku Noramalina Yusof, Nor Azah Abdullah, Jaafar Othman, Siti Sarah Issa, Rahizan Md Noh, Mohd Fairulnizal Wasoh, Helmi Death from tuberculosis has resulted in an increased need for early detection to prevent a tuberculosis (TB) epidemic, especially in closed and crowded populations. Herein, a sensitive electrochemical DNA biosensor based on functionalized iron oxide with mercaptopropionic acid (MPA-Fe3O4) nanoparticle and nanocellulose crystalline functionalized cetyl trimethyl ammonium bromide (NCC/CTAB) has been fabricated for the detection of Mycobacterium tuberculosis (MTB). In this study, a simple drop cast method was applied to deposit solution of MPA-Fe3O4/NCC/CTAB onto the surface of the screen-printed carbon electrode (SPCE). Then, a specific sequence of MTB DNA probe was immobilized onto a modified SPCE surface by using the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling mechanism. For better signal amplification and electrochemical response, ruthenium bipyridyl Ru(bpy)32+ was assigned as labels of hybridization followed by the characteristic test using differential pulse voltammetry (DPV). The results of this biosensor enable the detection of target DNA until a concentration as low as 7.96 × 10−13 M with a wide detection range from 1.0 × 10−6 to 1.0 × 10−12 M. In addition, the developed biosensor has shown a differentiation between positive and negative MTB samples in real sampel analysis. Multidisciplinary Digital Publishing Institute 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88498/1/ABSTRACT.pdf Mat Zaid, Mohd Hazani and Che-Engku-Chik, Che Engku Noramalina and Yusof, Nor Azah and Abdullah, Jaafar and Othman, Siti Sarah and Issa, Rahizan and Md Noh, Mohd Fairulnizal and Wasoh, Helmi (2020) DNA electrochemical biosensor based on iron oxide/nanocellulose crystalline composite modified screen-printed carbon electrode for detection of Mycobacterium tuberculosis. Molecules, 25 (15). art. no. 3373. pp. 1-14. ISSN 1420-3049 https://www.mdpi.com/1420-3049/25/15/3373 10.3390/molecules25153373 |
| spellingShingle | Mat Zaid, Mohd Hazani Che-Engku-Chik, Che Engku Noramalina Yusof, Nor Azah Abdullah, Jaafar Othman, Siti Sarah Issa, Rahizan Md Noh, Mohd Fairulnizal Wasoh, Helmi DNA electrochemical biosensor based on iron oxide/nanocellulose crystalline composite modified screen-printed carbon electrode for detection of Mycobacterium tuberculosis |
| title | DNA electrochemical biosensor based on iron oxide/nanocellulose crystalline composite modified screen-printed carbon electrode for detection of Mycobacterium tuberculosis |
| title_full | DNA electrochemical biosensor based on iron oxide/nanocellulose crystalline composite modified screen-printed carbon electrode for detection of Mycobacterium tuberculosis |
| title_fullStr | DNA electrochemical biosensor based on iron oxide/nanocellulose crystalline composite modified screen-printed carbon electrode for detection of Mycobacterium tuberculosis |
| title_full_unstemmed | DNA electrochemical biosensor based on iron oxide/nanocellulose crystalline composite modified screen-printed carbon electrode for detection of Mycobacterium tuberculosis |
| title_short | DNA electrochemical biosensor based on iron oxide/nanocellulose crystalline composite modified screen-printed carbon electrode for detection of Mycobacterium tuberculosis |
| title_sort | dna electrochemical biosensor based on iron oxide nanocellulose crystalline composite modified screen printed carbon electrode for detection of mycobacterium tuberculosis |
| url | http://psasir.upm.edu.my/id/eprint/88498/1/ABSTRACT.pdf |
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