Effects of heavy metals on Antarctic bacterial cell growth kinetics and degradation of waste canola oil

Aim: The aim of the present study was to study the effect of heavy metals on growth kinetics of Antarctic bacterial in degradation of waste canola oil. Methodology: The BS14 Antarctic bacterial community was introduced in the minimal salt media containing 1 ppm of heavy metals (Cd, Cr, Al, Zn, Ni, A...

Full description

Bibliographic Details
Main Authors: Mohd Zahri, Khadijah Nabilah, Zulkharnain, Azham, Gomez-Fuentes, Claudio, Sabri, Suriana, Ahmad, Siti Aqlima
Format: Article
Language:English
Published: Triveni Enterprises 2020
Online Access:http://psasir.upm.edu.my/id/eprint/88709/1/ABSTRACT.pdf
_version_ 1825936559077064704
author Mohd Zahri, Khadijah Nabilah
Zulkharnain, Azham
Gomez-Fuentes, Claudio
Sabri, Suriana
Ahmad, Siti Aqlima
author_facet Mohd Zahri, Khadijah Nabilah
Zulkharnain, Azham
Gomez-Fuentes, Claudio
Sabri, Suriana
Ahmad, Siti Aqlima
author_sort Mohd Zahri, Khadijah Nabilah
collection UPM
description Aim: The aim of the present study was to study the effect of heavy metals on growth kinetics of Antarctic bacterial in degradation of waste canola oil. Methodology: The BS14 Antarctic bacterial community was introduced in the minimal salt media containing 1 ppm of heavy metals (Cd, Cr, Al, Zn, Ni, As and Co) with 1% waste canola oil, and the effects of heavy metals on biodegradation of waste canola oil was analysed by gravimetric analysis. The turbidity of bacteria was obtained through UV-visible spectroscopy at 600 nm of wavelength for every 24 hr within seven days of incubation period, and the data were regressed with linear and non-linear kinetic equations. Results: The results demonstrated that Co was the most active metal that led to 4.217% increase in waste canola oil and the least active metal in biodegradation of oil was zinc, as it degraded the waste canola oil only to 29.26%. Overall, the bacterial growth was inhibited in increasing order of Al > Cd> As> Zn> Ni> Cr> Co whereas the waste canola oil biodegradation was inhibited in the order of Zn> Cr> Ni> Al> Cd> As> Co. The best fittedregression model was determined by comparing the kinetic parameters 2 estimated between linear and non-linear model equations, where the R value for non-linear regression was highest at 0.8421, and low sy.x at 0.324 for Ni -1 with a maximum growth rate (0.01131 hr ) of the Antarctic bacterial in degrading waste canola oil, meantime best-fitted in the linear regression 2 - model was Zn with high R and growth constant values (0.9082 and 0.2075 hr 1, respectively) as well as low value of statistical error, which was 0.2075. Interpretation: The presence of heavy metals in Antarctic bacterial community could suppress the ability of bacteria to degrade waste canola oil, and this can slower the rate of bacterial growth in the kinetics studies. Hence, this work would be helpful in actual bioremediation operations by understanding and manipulating the process of the kinetics parameters.
first_indexed 2024-03-06T10:46:07Z
format Article
id upm.eprints-88709
institution Universiti Putra Malaysia
language English
last_indexed 2024-03-06T10:46:07Z
publishDate 2020
publisher Triveni Enterprises
record_format dspace
spelling upm.eprints-887092021-11-03T05:27:25Z http://psasir.upm.edu.my/id/eprint/88709/ Effects of heavy metals on Antarctic bacterial cell growth kinetics and degradation of waste canola oil Mohd Zahri, Khadijah Nabilah Zulkharnain, Azham Gomez-Fuentes, Claudio Sabri, Suriana Ahmad, Siti Aqlima Aim: The aim of the present study was to study the effect of heavy metals on growth kinetics of Antarctic bacterial in degradation of waste canola oil. Methodology: The BS14 Antarctic bacterial community was introduced in the minimal salt media containing 1 ppm of heavy metals (Cd, Cr, Al, Zn, Ni, As and Co) with 1% waste canola oil, and the effects of heavy metals on biodegradation of waste canola oil was analysed by gravimetric analysis. The turbidity of bacteria was obtained through UV-visible spectroscopy at 600 nm of wavelength for every 24 hr within seven days of incubation period, and the data were regressed with linear and non-linear kinetic equations. Results: The results demonstrated that Co was the most active metal that led to 4.217% increase in waste canola oil and the least active metal in biodegradation of oil was zinc, as it degraded the waste canola oil only to 29.26%. Overall, the bacterial growth was inhibited in increasing order of Al > Cd> As> Zn> Ni> Cr> Co whereas the waste canola oil biodegradation was inhibited in the order of Zn> Cr> Ni> Al> Cd> As> Co. The best fittedregression model was determined by comparing the kinetic parameters 2 estimated between linear and non-linear model equations, where the R value for non-linear regression was highest at 0.8421, and low sy.x at 0.324 for Ni -1 with a maximum growth rate (0.01131 hr ) of the Antarctic bacterial in degrading waste canola oil, meantime best-fitted in the linear regression 2 - model was Zn with high R and growth constant values (0.9082 and 0.2075 hr 1, respectively) as well as low value of statistical error, which was 0.2075. Interpretation: The presence of heavy metals in Antarctic bacterial community could suppress the ability of bacteria to degrade waste canola oil, and this can slower the rate of bacterial growth in the kinetics studies. Hence, this work would be helpful in actual bioremediation operations by understanding and manipulating the process of the kinetics parameters. Triveni Enterprises 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88709/1/ABSTRACT.pdf Mohd Zahri, Khadijah Nabilah and Zulkharnain, Azham and Gomez-Fuentes, Claudio and Sabri, Suriana and Ahmad, Siti Aqlima (2020) Effects of heavy metals on Antarctic bacterial cell growth kinetics and degradation of waste canola oil. Journal of Environmental Biology, 41 (6). 1433 - 1441. ISSN 0254-8704; ESSN: 2394-0379 http://www.jeb.co.in/index.php?page=issue_toc&issue=202011_nov20 10.22438/JEB/41/6/MRN-1464
spellingShingle Mohd Zahri, Khadijah Nabilah
Zulkharnain, Azham
Gomez-Fuentes, Claudio
Sabri, Suriana
Ahmad, Siti Aqlima
Effects of heavy metals on Antarctic bacterial cell growth kinetics and degradation of waste canola oil
title Effects of heavy metals on Antarctic bacterial cell growth kinetics and degradation of waste canola oil
title_full Effects of heavy metals on Antarctic bacterial cell growth kinetics and degradation of waste canola oil
title_fullStr Effects of heavy metals on Antarctic bacterial cell growth kinetics and degradation of waste canola oil
title_full_unstemmed Effects of heavy metals on Antarctic bacterial cell growth kinetics and degradation of waste canola oil
title_short Effects of heavy metals on Antarctic bacterial cell growth kinetics and degradation of waste canola oil
title_sort effects of heavy metals on antarctic bacterial cell growth kinetics and degradation of waste canola oil
url http://psasir.upm.edu.my/id/eprint/88709/1/ABSTRACT.pdf
work_keys_str_mv AT mohdzahrikhadijahnabilah effectsofheavymetalsonantarcticbacterialcellgrowthkineticsanddegradationofwastecanolaoil
AT zulkharnainazham effectsofheavymetalsonantarcticbacterialcellgrowthkineticsanddegradationofwastecanolaoil
AT gomezfuentesclaudio effectsofheavymetalsonantarcticbacterialcellgrowthkineticsanddegradationofwastecanolaoil
AT sabrisuriana effectsofheavymetalsonantarcticbacterialcellgrowthkineticsanddegradationofwastecanolaoil
AT ahmadsitiaqlima effectsofheavymetalsonantarcticbacterialcellgrowthkineticsanddegradationofwastecanolaoil