Electrotransformation on selected plant growth-promoting Rhizobacteria (PGPR) and Chromobacterium violaceum
Electrotransformation was successfully transferred a 21.6 kb cosmid vector pLAFR1 into the selected Plant Growth-Promoting Rhizobacteria (PGPR) and Chromobacterium violaceum with maximum efficiency about 107 transformants/µg at electric field strength of 10kV/cm for shorter pulse length range betwee...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Sultan Zainal Abidin
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/88779/1/ABSTRACT.pdf |
| _version_ | 1825936570578894848 |
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| author | Loke, Wai Keong Mohd Saud, Halimi |
| author_facet | Loke, Wai Keong Mohd Saud, Halimi |
| author_sort | Loke, Wai Keong |
| collection | UPM |
| description | Electrotransformation was successfully transferred a 21.6 kb cosmid vector pLAFR1 into the selected Plant Growth-Promoting Rhizobacteria (PGPR) and Chromobacterium violaceum with maximum efficiency about 107 transformants/µg at electric field strength of 10kV/cm for shorter pulse length range between 4.1 to 4.4 milliseconds and about 108 transformants/µg at electric field strength of 5kV/cm for a longer pulse length range between 8.5 to 8.7 milliseconds. Longer incubation period will increase the transformation efficiency but continuing increasing the period after 20 hours will decrease the transformation efficiency. |
| first_indexed | 2024-03-06T10:46:18Z |
| format | Article |
| id | upm.eprints-88779 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:46:18Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Sultan Zainal Abidin |
| record_format | dspace |
| spelling | upm.eprints-887792021-11-03T03:53:49Z http://psasir.upm.edu.my/id/eprint/88779/ Electrotransformation on selected plant growth-promoting Rhizobacteria (PGPR) and Chromobacterium violaceum Loke, Wai Keong Mohd Saud, Halimi Electrotransformation was successfully transferred a 21.6 kb cosmid vector pLAFR1 into the selected Plant Growth-Promoting Rhizobacteria (PGPR) and Chromobacterium violaceum with maximum efficiency about 107 transformants/µg at electric field strength of 10kV/cm for shorter pulse length range between 4.1 to 4.4 milliseconds and about 108 transformants/µg at electric field strength of 5kV/cm for a longer pulse length range between 8.5 to 8.7 milliseconds. Longer incubation period will increase the transformation efficiency but continuing increasing the period after 20 hours will decrease the transformation efficiency. Penerbit Universiti Sultan Zainal Abidin 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88779/1/ABSTRACT.pdf Loke, Wai Keong and Mohd Saud, Halimi (2020) Electrotransformation on selected plant growth-promoting Rhizobacteria (PGPR) and Chromobacterium violaceum. Journal of Agrobiotechnology, 11 (1). 63 - 69. ISSN 2180-1983 https://journal.unisza.edu.my/agrobiotechnology/index.php/agrobiotechnology/article/view/214 10.37231/jab.2020.11.1.214 |
| spellingShingle | Loke, Wai Keong Mohd Saud, Halimi Electrotransformation on selected plant growth-promoting Rhizobacteria (PGPR) and Chromobacterium violaceum |
| title | Electrotransformation on selected plant growth-promoting Rhizobacteria (PGPR) and Chromobacterium violaceum |
| title_full | Electrotransformation on selected plant growth-promoting Rhizobacteria (PGPR) and Chromobacterium violaceum |
| title_fullStr | Electrotransformation on selected plant growth-promoting Rhizobacteria (PGPR) and Chromobacterium violaceum |
| title_full_unstemmed | Electrotransformation on selected plant growth-promoting Rhizobacteria (PGPR) and Chromobacterium violaceum |
| title_short | Electrotransformation on selected plant growth-promoting Rhizobacteria (PGPR) and Chromobacterium violaceum |
| title_sort | electrotransformation on selected plant growth promoting rhizobacteria pgpr and chromobacterium violaceum |
| url | http://psasir.upm.edu.my/id/eprint/88779/1/ABSTRACT.pdf |
| work_keys_str_mv | AT lokewaikeong electrotransformationonselectedplantgrowthpromotingrhizobacteriapgprandchromobacteriumviolaceum AT mohdsaudhalimi electrotransformationonselectedplantgrowthpromotingrhizobacteriapgprandchromobacteriumviolaceum |