Evaluation of acute toxicity of an aqueous extract of irradiated Labisia pumila on zebrafish embryo (Danio Rerio)
This research aimed to compare the toxicity effect of non-irradiated and irradiated Labisia pumila at a different dosage of 3, 6, 9 and 12 kilogray (kGy). Different irradiated dosages of L.pumila were prepared using Cobalt-60 gamma irradiation and the acute toxicity were assessed through zebrafish (...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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All India Institute of Medical Sciences
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/88862/1/ABSTRACT.pdf |
| _version_ | 1825936583080017920 |
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| author | Balan, Santhra Segaran Manokaram, Punithaamalar Shari, Norshafarina Mohd Uzid, Mahathir Bahari, Hasnah Che Ramli, Muhammad Danial Zainal Abidin, Azrina Rahmadi, Mahardian Arapoc, Daryl Jesus |
| author_facet | Balan, Santhra Segaran Manokaram, Punithaamalar Shari, Norshafarina Mohd Uzid, Mahathir Bahari, Hasnah Che Ramli, Muhammad Danial Zainal Abidin, Azrina Rahmadi, Mahardian Arapoc, Daryl Jesus |
| author_sort | Balan, Santhra Segaran |
| collection | UPM |
| description | This research aimed to compare the toxicity effect of non-irradiated and irradiated Labisia pumila at a different dosage of 3, 6, 9 and 12 kilogray (kGy). Different irradiated dosages of L.pumila were prepared using Cobalt-60 gamma irradiation and the acute toxicity were assessed through zebrafish (Danio rerio) embryo. The survival rate, hatching rate, heartbeat rate and scoliosis were observed. Data were analyzed using SPSS 25.0 windows. The lethal dose (LC50) value was calculated. The LC50 value of non- irradiated extract L. pumila is 125 μg/ml compared to irradiated extract is 62.5 μg/ml respectively. Hatchability of zebrafish of L.pumila extract reduce in the higher concentration for non-irradiated sample at 250 μg/ml and for irradiated sample at 125 μg/ml. Presence of scoliosis not observed in all concentration for irradiated and non-irradiated sample. The heartbeat of zebrafish embryo treated with irradiated L. pumila extract (0–62.5 μg/ml) was within the normal range (120–180 bpm for all doses), but at higher concentrations (125 μg/ml) the heartbeat differs from normal ranges for all the doses. From this time forward, irradiated and non-irradiated of this plant was safe to be consumed due to its pharmaceutical effect but it still exhibited mild toxicity effect on zebrafish embryo. The diverse irradiated doses show a change of toxicity level of this plant which higher doses show mild toxicity to the zebrafish embryo compared to low doses exposure. |
| first_indexed | 2024-03-06T10:46:29Z |
| format | Article |
| id | upm.eprints-88862 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:46:29Z |
| publishDate | 2020 |
| publisher | All India Institute of Medical Sciences |
| record_format | dspace |
| spelling | upm.eprints-888622021-11-02T20:06:05Z http://psasir.upm.edu.my/id/eprint/88862/ Evaluation of acute toxicity of an aqueous extract of irradiated Labisia pumila on zebrafish embryo (Danio Rerio) Balan, Santhra Segaran Manokaram, Punithaamalar Shari, Norshafarina Mohd Uzid, Mahathir Bahari, Hasnah Che Ramli, Muhammad Danial Zainal Abidin, Azrina Rahmadi, Mahardian Arapoc, Daryl Jesus This research aimed to compare the toxicity effect of non-irradiated and irradiated Labisia pumila at a different dosage of 3, 6, 9 and 12 kilogray (kGy). Different irradiated dosages of L.pumila were prepared using Cobalt-60 gamma irradiation and the acute toxicity were assessed through zebrafish (Danio rerio) embryo. The survival rate, hatching rate, heartbeat rate and scoliosis were observed. Data were analyzed using SPSS 25.0 windows. The lethal dose (LC50) value was calculated. The LC50 value of non- irradiated extract L. pumila is 125 μg/ml compared to irradiated extract is 62.5 μg/ml respectively. Hatchability of zebrafish of L.pumila extract reduce in the higher concentration for non-irradiated sample at 250 μg/ml and for irradiated sample at 125 μg/ml. Presence of scoliosis not observed in all concentration for irradiated and non-irradiated sample. The heartbeat of zebrafish embryo treated with irradiated L. pumila extract (0–62.5 μg/ml) was within the normal range (120–180 bpm for all doses), but at higher concentrations (125 μg/ml) the heartbeat differs from normal ranges for all the doses. From this time forward, irradiated and non-irradiated of this plant was safe to be consumed due to its pharmaceutical effect but it still exhibited mild toxicity effect on zebrafish embryo. The diverse irradiated doses show a change of toxicity level of this plant which higher doses show mild toxicity to the zebrafish embryo compared to low doses exposure. All India Institute of Medical Sciences 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88862/1/ABSTRACT.pdf Balan, Santhra Segaran and Manokaram, Punithaamalar and Shari, Norshafarina and Mohd Uzid, Mahathir and Bahari, Hasnah and Che Ramli, Muhammad Danial and Zainal Abidin, Azrina and Rahmadi, Mahardian and Arapoc, Daryl Jesus (2020) Evaluation of acute toxicity of an aqueous extract of irradiated Labisia pumila on zebrafish embryo (Danio Rerio). International Journal of Medical Toxicology and Legal Medicine, 23 (1 & 2). 42 - 46. ISSN 0972-0448; ESSN: 0974-4614 https://www.indianjournals.com/ijor.aspx?target=ijor:ijmtlm&volume=23&issue=1and2&article=008 10.5958/0974-4614.2020.00008.X |
| spellingShingle | Balan, Santhra Segaran Manokaram, Punithaamalar Shari, Norshafarina Mohd Uzid, Mahathir Bahari, Hasnah Che Ramli, Muhammad Danial Zainal Abidin, Azrina Rahmadi, Mahardian Arapoc, Daryl Jesus Evaluation of acute toxicity of an aqueous extract of irradiated Labisia pumila on zebrafish embryo (Danio Rerio) |
| title | Evaluation of acute toxicity of an aqueous extract of irradiated Labisia pumila on zebrafish embryo (Danio Rerio) |
| title_full | Evaluation of acute toxicity of an aqueous extract of irradiated Labisia pumila on zebrafish embryo (Danio Rerio) |
| title_fullStr | Evaluation of acute toxicity of an aqueous extract of irradiated Labisia pumila on zebrafish embryo (Danio Rerio) |
| title_full_unstemmed | Evaluation of acute toxicity of an aqueous extract of irradiated Labisia pumila on zebrafish embryo (Danio Rerio) |
| title_short | Evaluation of acute toxicity of an aqueous extract of irradiated Labisia pumila on zebrafish embryo (Danio Rerio) |
| title_sort | evaluation of acute toxicity of an aqueous extract of irradiated labisia pumila on zebrafish embryo danio rerio |
| url | http://psasir.upm.edu.my/id/eprint/88862/1/ABSTRACT.pdf |
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