Evaluation of agro-based waste substrates for micropropagule formation in biocontrol fungi, Trichoderma asperellum and T. harzianum

Trichoderma species have shown efficiency on biocontrol of phytopathogens. For commercial application, it must be propagated in mass scale using a cost-effective method. As an alternative way to effectively deliver biocontrol fungi inoculum to the field; seven agro-based wastes including rice bran,...

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Main Authors: Hasan, Z. A. E., Mohd Zainudin, Nur Ain Izzati, Aris, A., Ibrahim, Mohd Hafiz, Yusof, Mohd Termizi
Format: Article
Language:English
Published: Maximum Academic Press 2020
Online Access:http://psasir.upm.edu.my/id/eprint/88863/1/ABSTRACT.pdf
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author Hasan, Z. A. E.
Mohd Zainudin, Nur Ain Izzati
Aris, A.
Ibrahim, Mohd Hafiz
Yusof, Mohd Termizi
author_facet Hasan, Z. A. E.
Mohd Zainudin, Nur Ain Izzati
Aris, A.
Ibrahim, Mohd Hafiz
Yusof, Mohd Termizi
author_sort Hasan, Z. A. E.
collection UPM
description Trichoderma species have shown efficiency on biocontrol of phytopathogens. For commercial application, it must be propagated in mass scale using a cost-effective method. As an alternative way to effectively deliver biocontrol fungi inoculum to the field; seven agro-based wastes including rice bran, biochar, empty fruit bunches, coconut fibre, compost, topsoil and mixed soil were used in this study for evaluating mass multiplication of Trichoderma species. Based on the evaluation of colony-forming units (cfu) among the agro-based waste media used, coconut fibre is the most suitable in promoting the sporulation of Trichoderma asperellum and T. harzianum. Trichoderma asperellum C1667 showed the higher micropropagule count through incubation period compared to T. harzianum C1675. After 120 days on the agro-based waste media, T. asperellum C1667 and T. harzianum C1675 produced the highest (7.717×105 cfu/g and 6.836±13.79×105 cfu/g) coconut fibres, respectively. Meanwhile, the mixed soil appeared with the lowest cfu. Coconut fibres were shown as a great biocomposting medium for both Trichoderma species. Findings of the present study are valuable for disease management using agro-based wastes as a cost-effective medium for biocontrol agents like Trichoderma species.
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spelling upm.eprints-888632021-11-03T05:55:20Z http://psasir.upm.edu.my/id/eprint/88863/ Evaluation of agro-based waste substrates for micropropagule formation in biocontrol fungi, Trichoderma asperellum and T. harzianum Hasan, Z. A. E. Mohd Zainudin, Nur Ain Izzati Aris, A. Ibrahim, Mohd Hafiz Yusof, Mohd Termizi Trichoderma species have shown efficiency on biocontrol of phytopathogens. For commercial application, it must be propagated in mass scale using a cost-effective method. As an alternative way to effectively deliver biocontrol fungi inoculum to the field; seven agro-based wastes including rice bran, biochar, empty fruit bunches, coconut fibre, compost, topsoil and mixed soil were used in this study for evaluating mass multiplication of Trichoderma species. Based on the evaluation of colony-forming units (cfu) among the agro-based waste media used, coconut fibre is the most suitable in promoting the sporulation of Trichoderma asperellum and T. harzianum. Trichoderma asperellum C1667 showed the higher micropropagule count through incubation period compared to T. harzianum C1675. After 120 days on the agro-based waste media, T. asperellum C1667 and T. harzianum C1675 produced the highest (7.717×105 cfu/g and 6.836±13.79×105 cfu/g) coconut fibres, respectively. Meanwhile, the mixed soil appeared with the lowest cfu. Coconut fibres were shown as a great biocomposting medium for both Trichoderma species. Findings of the present study are valuable for disease management using agro-based wastes as a cost-effective medium for biocontrol agents like Trichoderma species. Maximum Academic Press 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88863/1/ABSTRACT.pdf Hasan, Z. A. E. and Mohd Zainudin, Nur Ain Izzati and Aris, A. and Ibrahim, Mohd Hafiz and Yusof, Mohd Termizi (2020) Evaluation of agro-based waste substrates for micropropagule formation in biocontrol fungi, Trichoderma asperellum and T. harzianum. Studies in Fungi, 5 (1). 94 - 102. ISSN 2465-4973 http://www.maxapress.com/article/id/be66deee-c45f-4b17-939a-65e85f9ea976 10.5943/sif/5/1/9
spellingShingle Hasan, Z. A. E.
Mohd Zainudin, Nur Ain Izzati
Aris, A.
Ibrahim, Mohd Hafiz
Yusof, Mohd Termizi
Evaluation of agro-based waste substrates for micropropagule formation in biocontrol fungi, Trichoderma asperellum and T. harzianum
title Evaluation of agro-based waste substrates for micropropagule formation in biocontrol fungi, Trichoderma asperellum and T. harzianum
title_full Evaluation of agro-based waste substrates for micropropagule formation in biocontrol fungi, Trichoderma asperellum and T. harzianum
title_fullStr Evaluation of agro-based waste substrates for micropropagule formation in biocontrol fungi, Trichoderma asperellum and T. harzianum
title_full_unstemmed Evaluation of agro-based waste substrates for micropropagule formation in biocontrol fungi, Trichoderma asperellum and T. harzianum
title_short Evaluation of agro-based waste substrates for micropropagule formation in biocontrol fungi, Trichoderma asperellum and T. harzianum
title_sort evaluation of agro based waste substrates for micropropagule formation in biocontrol fungi trichoderma asperellum and t harzianum
url http://psasir.upm.edu.my/id/eprint/88863/1/ABSTRACT.pdf
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