Experimental evaluation on surface plasmon resonance sensor performance based on sensitive hyperbranched polymer nanocomposite thin films
The plethora of optical techniques have been developed to detect the dengue virus, regrettably, the existing techniques are cumbersome and have limitations to provide early identification of the low-level of dengue virus. For this purpose, surface plasmon resonance (SPR) optical sensor was designed...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/88896/1/CIP.pdf |
| _version_ | 1796982046770331648 |
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| author | Sheh Omar, Nur Alia Fen, Yap Wing Saleviter, Silvan Mustapha Kamil, Yasmin Mohd Daniyal, Wan Mohd Ebtisyam Mustaqim Abdullah, Jaafar Mahdi, Mohd Adzir |
| author_facet | Sheh Omar, Nur Alia Fen, Yap Wing Saleviter, Silvan Mustapha Kamil, Yasmin Mohd Daniyal, Wan Mohd Ebtisyam Mustaqim Abdullah, Jaafar Mahdi, Mohd Adzir |
| author_sort | Sheh Omar, Nur Alia |
| collection | UPM |
| description | The plethora of optical techniques have been developed to detect the dengue virus, regrettably, the existing techniques are cumbersome and have limitations to provide early identification of the low-level of dengue virus. For this purpose, surface plasmon resonance (SPR) optical sensor was designed with a highly sensitive thin film based on hyperbranched polymer nanocomposites for conjugation of antibodies that specifically detect the dengue virus type II (DENV). In particular, we investigate the SPR sensing performance for gold/cadmium sulfide quantum dots-polyamidoamine/antibody (Au/CdS QDs-PAMAM/IgM) sensor film in terms of linear sensitivity, binding affinity, detection accuracy, full-width-half-maximum, selectivity, and spike and recovery test. The results show a strong affinity value, good sensitivity and selectivity, high accuracy, and excellent recovery value, obeying the ideal value based on conventional assay analysis. Additionally, this Au/CdS QDs-PAMAM/IgM based SPR sensor was successfully applied for the lowest detection of 1 pM of DENV E-proteins. To this end, a highly sensitive Au/CdS QDs-PAMAM/IgM sensor film was characterized using Raman spectroscopy, ultraviolet-visible (UV–Vis-NIR) spectroscopy, and atomic force microscopy (AFM) to confirm the binding of DENV. |
| first_indexed | 2024-03-06T10:46:34Z |
| format | Article |
| id | upm.eprints-88896 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:46:34Z |
| publishDate | 2020 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-888962021-10-04T23:39:46Z http://psasir.upm.edu.my/id/eprint/88896/ Experimental evaluation on surface plasmon resonance sensor performance based on sensitive hyperbranched polymer nanocomposite thin films Sheh Omar, Nur Alia Fen, Yap Wing Saleviter, Silvan Mustapha Kamil, Yasmin Mohd Daniyal, Wan Mohd Ebtisyam Mustaqim Abdullah, Jaafar Mahdi, Mohd Adzir The plethora of optical techniques have been developed to detect the dengue virus, regrettably, the existing techniques are cumbersome and have limitations to provide early identification of the low-level of dengue virus. For this purpose, surface plasmon resonance (SPR) optical sensor was designed with a highly sensitive thin film based on hyperbranched polymer nanocomposites for conjugation of antibodies that specifically detect the dengue virus type II (DENV). In particular, we investigate the SPR sensing performance for gold/cadmium sulfide quantum dots-polyamidoamine/antibody (Au/CdS QDs-PAMAM/IgM) sensor film in terms of linear sensitivity, binding affinity, detection accuracy, full-width-half-maximum, selectivity, and spike and recovery test. The results show a strong affinity value, good sensitivity and selectivity, high accuracy, and excellent recovery value, obeying the ideal value based on conventional assay analysis. Additionally, this Au/CdS QDs-PAMAM/IgM based SPR sensor was successfully applied for the lowest detection of 1 pM of DENV E-proteins. To this end, a highly sensitive Au/CdS QDs-PAMAM/IgM sensor film was characterized using Raman spectroscopy, ultraviolet-visible (UV–Vis-NIR) spectroscopy, and atomic force microscopy (AFM) to confirm the binding of DENV. Elsevier 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88896/1/CIP.pdf Sheh Omar, Nur Alia and Fen, Yap Wing and Saleviter, Silvan and Mustapha Kamil, Yasmin and Mohd Daniyal, Wan Mohd Ebtisyam Mustaqim and Abdullah, Jaafar and Mahdi, Mohd Adzir (2020) Experimental evaluation on surface plasmon resonance sensor performance based on sensitive hyperbranched polymer nanocomposite thin films. Sensors and Actuators, A: Physical, 303. art. no. 111830. pp. 1-10. ISSN 0924-4247 https://www.sciencedirect.com/science/article/pii/S0924424719315493 10.1016/j.sna.2020.111830 |
| spellingShingle | Sheh Omar, Nur Alia Fen, Yap Wing Saleviter, Silvan Mustapha Kamil, Yasmin Mohd Daniyal, Wan Mohd Ebtisyam Mustaqim Abdullah, Jaafar Mahdi, Mohd Adzir Experimental evaluation on surface plasmon resonance sensor performance based on sensitive hyperbranched polymer nanocomposite thin films |
| title | Experimental evaluation on surface plasmon resonance sensor performance based on sensitive hyperbranched polymer nanocomposite thin films |
| title_full | Experimental evaluation on surface plasmon resonance sensor performance based on sensitive hyperbranched polymer nanocomposite thin films |
| title_fullStr | Experimental evaluation on surface plasmon resonance sensor performance based on sensitive hyperbranched polymer nanocomposite thin films |
| title_full_unstemmed | Experimental evaluation on surface plasmon resonance sensor performance based on sensitive hyperbranched polymer nanocomposite thin films |
| title_short | Experimental evaluation on surface plasmon resonance sensor performance based on sensitive hyperbranched polymer nanocomposite thin films |
| title_sort | experimental evaluation on surface plasmon resonance sensor performance based on sensitive hyperbranched polymer nanocomposite thin films |
| url | http://psasir.upm.edu.my/id/eprint/88896/1/CIP.pdf |
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