Exposure to Zoonotic West Nile Virus in long-tailed macaques and bats in Peninsular Malaysia
The role of wildlife such as wild birds, macaques, and bats in the spreading and maintenance of deadly zoonotic pathogens in nature have been well documented in many parts of the world. One such pathogen is the mosquitoes borne virus, namely the West Nile Virus (WNV). Previous research has shown tha...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Multidisciplinary Digital Publishing Institute
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/88931/1/WEST.pdf |
| _version_ | 1825936595143884800 |
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| author | Mohd Yuseri, Nur Ain Najwa Abd Rahaman, Nor Yasmin Arshad, Siti Suri Omar, Abdul Rahman Abu, Jalila Kumar, Kiven Mohammed, Hussni O. Jafar Ali, Natasha Mohammed, Mohammed Nma Bande, Faruku Abdullah, Mohd Lutfi Rovie-Ryan, Jeffrine J. |
| author_facet | Mohd Yuseri, Nur Ain Najwa Abd Rahaman, Nor Yasmin Arshad, Siti Suri Omar, Abdul Rahman Abu, Jalila Kumar, Kiven Mohammed, Hussni O. Jafar Ali, Natasha Mohammed, Mohammed Nma Bande, Faruku Abdullah, Mohd Lutfi Rovie-Ryan, Jeffrine J. |
| author_sort | Mohd Yuseri, Nur Ain Najwa |
| collection | UPM |
| description | The role of wildlife such as wild birds, macaques, and bats in the spreading and maintenance of deadly zoonotic pathogens in nature have been well documented in many parts of the world. One such pathogen is the mosquitoes borne virus, namely the West Nile Virus (WNV). Previous research has shown that 1:7 and 1:6 Malaysian wild birds are WNV antibody and RNA positive, respectively, and bats in North America may not be susceptible to the WNV infection. This study was conducted to determine the status of WNV in Malaysian macaques and bats found in mangrove forests and caves, respectively. Archive sera and oropharyngeal swabs from long-tailed macaques were subjected to the antibody detection using WNV competitive enzyme-linked immunosorbent assay (c-ELISA) and WNV RNA using RT-PCR, respectively, while the archive oropharyngeal and rectal swabs from bats were subjected to RT-PCR without serological analysis due to the unavailability of serum samples. The analysis revealed a WNV seropositivity of 29.63% (24/81) and none of the macaques were positive for WNV RNA. Meanwhile, 12.2% (5/41) of the bats from Pteropodidae, Emballonuridae, and Rhinolophidae families tested positive for WNV RNA. Here, we show a high WNV antibody prevalence in macaques and a moderate WNV RNA in various Malaysian bat species, suggesting that WNV circulates through Malaysian wild animals and Malaysian bat species may be susceptible to the WNV infection. |
| first_indexed | 2024-03-06T10:46:40Z |
| format | Article |
| id | upm.eprints-88931 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:46:40Z |
| publishDate | 2020 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | upm.eprints-889312021-10-04T22:22:13Z http://psasir.upm.edu.my/id/eprint/88931/ Exposure to Zoonotic West Nile Virus in long-tailed macaques and bats in Peninsular Malaysia Mohd Yuseri, Nur Ain Najwa Abd Rahaman, Nor Yasmin Arshad, Siti Suri Omar, Abdul Rahman Abu, Jalila Kumar, Kiven Mohammed, Hussni O. Jafar Ali, Natasha Mohammed, Mohammed Nma Bande, Faruku Abdullah, Mohd Lutfi Rovie-Ryan, Jeffrine J. The role of wildlife such as wild birds, macaques, and bats in the spreading and maintenance of deadly zoonotic pathogens in nature have been well documented in many parts of the world. One such pathogen is the mosquitoes borne virus, namely the West Nile Virus (WNV). Previous research has shown that 1:7 and 1:6 Malaysian wild birds are WNV antibody and RNA positive, respectively, and bats in North America may not be susceptible to the WNV infection. This study was conducted to determine the status of WNV in Malaysian macaques and bats found in mangrove forests and caves, respectively. Archive sera and oropharyngeal swabs from long-tailed macaques were subjected to the antibody detection using WNV competitive enzyme-linked immunosorbent assay (c-ELISA) and WNV RNA using RT-PCR, respectively, while the archive oropharyngeal and rectal swabs from bats were subjected to RT-PCR without serological analysis due to the unavailability of serum samples. The analysis revealed a WNV seropositivity of 29.63% (24/81) and none of the macaques were positive for WNV RNA. Meanwhile, 12.2% (5/41) of the bats from Pteropodidae, Emballonuridae, and Rhinolophidae families tested positive for WNV RNA. Here, we show a high WNV antibody prevalence in macaques and a moderate WNV RNA in various Malaysian bat species, suggesting that WNV circulates through Malaysian wild animals and Malaysian bat species may be susceptible to the WNV infection. Multidisciplinary Digital Publishing Institute 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88931/1/WEST.pdf Mohd Yuseri, Nur Ain Najwa and Abd Rahaman, Nor Yasmin and Arshad, Siti Suri and Omar, Abdul Rahman and Abu, Jalila and Kumar, Kiven and Mohammed, Hussni O. and Jafar Ali, Natasha and Mohammed, Mohammed Nma and Bande, Faruku and Abdullah, Mohd Lutfi and Rovie-Ryan, Jeffrine J. (2020) Exposure to Zoonotic West Nile Virus in long-tailed macaques and bats in Peninsular Malaysia. Animals, 10 (12). art. no. 2367. pp. 1-13. ISSN 2076-2615 https://www.mdpi.com/2076-2615/10/12/2367 10.3390/ani10122367 |
| spellingShingle | Mohd Yuseri, Nur Ain Najwa Abd Rahaman, Nor Yasmin Arshad, Siti Suri Omar, Abdul Rahman Abu, Jalila Kumar, Kiven Mohammed, Hussni O. Jafar Ali, Natasha Mohammed, Mohammed Nma Bande, Faruku Abdullah, Mohd Lutfi Rovie-Ryan, Jeffrine J. Exposure to Zoonotic West Nile Virus in long-tailed macaques and bats in Peninsular Malaysia |
| title | Exposure to Zoonotic West Nile Virus in long-tailed macaques and bats in Peninsular Malaysia |
| title_full | Exposure to Zoonotic West Nile Virus in long-tailed macaques and bats in Peninsular Malaysia |
| title_fullStr | Exposure to Zoonotic West Nile Virus in long-tailed macaques and bats in Peninsular Malaysia |
| title_full_unstemmed | Exposure to Zoonotic West Nile Virus in long-tailed macaques and bats in Peninsular Malaysia |
| title_short | Exposure to Zoonotic West Nile Virus in long-tailed macaques and bats in Peninsular Malaysia |
| title_sort | exposure to zoonotic west nile virus in long tailed macaques and bats in peninsular malaysia |
| url | http://psasir.upm.edu.my/id/eprint/88931/1/WEST.pdf |
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