Fluorescence-based immunoassay for the detection of Xanthomonas oryzae pv. oryzae in rice leaf
The identification of rice bacterial leaf blight disease requires a simple, rapid, highly sensitive, and quantitative approach that can be applied as an early detection monitoring tool in rice health. This paper highlights the development of a turn-off fluorescence-based immunoassay for the early de...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/89049/1/DOT.pdf |
| _version_ | 1825936615117160448 |
|---|---|
| author | Awaludin, Norhafniza Abdullah, Jaafar Salam, Faridah Ramachandran, Kogeethavani Yusof, Nor Azah Wasoh, Helmi |
| author_facet | Awaludin, Norhafniza Abdullah, Jaafar Salam, Faridah Ramachandran, Kogeethavani Yusof, Nor Azah Wasoh, Helmi |
| author_sort | Awaludin, Norhafniza |
| collection | UPM |
| description | The identification of rice bacterial leaf blight disease requires a simple, rapid, highly sensitive, and quantitative approach that can be applied as an early detection monitoring tool in rice health. This paper highlights the development of a turn-off fluorescence-based immunoassay for the early detection of Xanthomonas oryzae pv. oryzae (Xoo), a gram-negative bacterium that causes rice bacterial leaf blight disease. Antibodies against Xoo bacterial cells were produced as specific bio-recognition molecules and the conjugation of these antibodies with graphene quantum dots and gold nanoparticles was performed and characterized, respectively. The combination of both these bio-probes as a fluorescent donor and metal quencher led to changes in the fluorescence signal. The immunoreaction between AntiXoo-GQDs, Xoo cells, and AntiXoo-AuNPs in the immuno-aggregation complex led to the energy transfer in the turn-off fluorescence-based quenching system. The change in fluorescence intensity was proportional to the logarithm of Xoo cells in the range of 100–105 CFU mL−1. The limit of detection was achieved at 22 CFU mL−1 and the specificity test against other plant disease pathogens showed high specificity towards Xoo. The detection of Xoo in real plant samples was also performed in this study and demonstrated satisfactory results. |
| first_indexed | 2024-03-06T10:46:58Z |
| format | Article |
| id | upm.eprints-89049 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:46:58Z |
| publishDate | 2020 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-890492021-09-21T10:00:02Z http://psasir.upm.edu.my/id/eprint/89049/ Fluorescence-based immunoassay for the detection of Xanthomonas oryzae pv. oryzae in rice leaf Awaludin, Norhafniza Abdullah, Jaafar Salam, Faridah Ramachandran, Kogeethavani Yusof, Nor Azah Wasoh, Helmi The identification of rice bacterial leaf blight disease requires a simple, rapid, highly sensitive, and quantitative approach that can be applied as an early detection monitoring tool in rice health. This paper highlights the development of a turn-off fluorescence-based immunoassay for the early detection of Xanthomonas oryzae pv. oryzae (Xoo), a gram-negative bacterium that causes rice bacterial leaf blight disease. Antibodies against Xoo bacterial cells were produced as specific bio-recognition molecules and the conjugation of these antibodies with graphene quantum dots and gold nanoparticles was performed and characterized, respectively. The combination of both these bio-probes as a fluorescent donor and metal quencher led to changes in the fluorescence signal. The immunoreaction between AntiXoo-GQDs, Xoo cells, and AntiXoo-AuNPs in the immuno-aggregation complex led to the energy transfer in the turn-off fluorescence-based quenching system. The change in fluorescence intensity was proportional to the logarithm of Xoo cells in the range of 100–105 CFU mL−1. The limit of detection was achieved at 22 CFU mL−1 and the specificity test against other plant disease pathogens showed high specificity towards Xoo. The detection of Xoo in real plant samples was also performed in this study and demonstrated satisfactory results. Elsevier 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/89049/1/DOT.pdf Awaludin, Norhafniza and Abdullah, Jaafar and Salam, Faridah and Ramachandran, Kogeethavani and Yusof, Nor Azah and Wasoh, Helmi (2020) Fluorescence-based immunoassay for the detection of Xanthomonas oryzae pv. oryzae in rice leaf. Analytical Biochemistry, 610. art. no. 113876. pp. 1-10. ISSN 0003-2697; ESSN: 1096-0309 https://www.sciencedirect.com/science/article/abs/pii/S0003269720304085 10.1016/j.ab.2020.113876 |
| spellingShingle | Awaludin, Norhafniza Abdullah, Jaafar Salam, Faridah Ramachandran, Kogeethavani Yusof, Nor Azah Wasoh, Helmi Fluorescence-based immunoassay for the detection of Xanthomonas oryzae pv. oryzae in rice leaf |
| title | Fluorescence-based immunoassay for the detection of Xanthomonas oryzae pv. oryzae in rice leaf |
| title_full | Fluorescence-based immunoassay for the detection of Xanthomonas oryzae pv. oryzae in rice leaf |
| title_fullStr | Fluorescence-based immunoassay for the detection of Xanthomonas oryzae pv. oryzae in rice leaf |
| title_full_unstemmed | Fluorescence-based immunoassay for the detection of Xanthomonas oryzae pv. oryzae in rice leaf |
| title_short | Fluorescence-based immunoassay for the detection of Xanthomonas oryzae pv. oryzae in rice leaf |
| title_sort | fluorescence based immunoassay for the detection of xanthomonas oryzae pv oryzae in rice leaf |
| url | http://psasir.upm.edu.my/id/eprint/89049/1/DOT.pdf |
| work_keys_str_mv | AT awaludinnorhafniza fluorescencebasedimmunoassayforthedetectionofxanthomonasoryzaepvoryzaeinriceleaf AT abdullahjaafar fluorescencebasedimmunoassayforthedetectionofxanthomonasoryzaepvoryzaeinriceleaf AT salamfaridah fluorescencebasedimmunoassayforthedetectionofxanthomonasoryzaepvoryzaeinriceleaf AT ramachandrankogeethavani fluorescencebasedimmunoassayforthedetectionofxanthomonasoryzaepvoryzaeinriceleaf AT yusofnorazah fluorescencebasedimmunoassayforthedetectionofxanthomonasoryzaepvoryzaeinriceleaf AT wasohhelmi fluorescencebasedimmunoassayforthedetectionofxanthomonasoryzaepvoryzaeinriceleaf |