Genome-wide identification and expression analysis of banana RBOH genes in response to Fusarium oxysporum f. sp. cubense Tropical Race 4
Respiratory burst oxidase homologs (RBOH) is the key enzyme responsible for the production of reactive oxygen species which act as important signal during plant responses to abiotic and biotic stresses. However, RBOH homologs have not been characterized in banana. In this study, we have identified t...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Malaysian Society for Biochemistry and Molecular Biology
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/89116/1/ABSTRACT.pdf |
| _version_ | 1796982084702568448 |
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| author | Chai, Siew Ying Tahir, Nur Fathiah Abu Bakar, Nur Syazana Teo, Chee How Mohd Yusoff, Nur Fatihah Baharum, Nadiya Akmal Saidi, Noor Baity |
| author_facet | Chai, Siew Ying Tahir, Nur Fathiah Abu Bakar, Nur Syazana Teo, Chee How Mohd Yusoff, Nur Fatihah Baharum, Nadiya Akmal Saidi, Noor Baity |
| author_sort | Chai, Siew Ying |
| collection | UPM |
| description | Respiratory burst oxidase homologs (RBOH) is the key enzyme responsible for the production of reactive oxygen species which act as important signal during plant responses to abiotic and biotic stresses. However, RBOH homologs have not been characterized in banana. In this study, we have identified twelve Rboh genes distributed on eight chromosomes of Musa acuminata subsp. malaccensis (DH Pahang) through a genome-wide analysis. MaRbohs exist as sibling paralogs with variable exon-intron
structures and highly conserved functional domain. Phylogenetic analysis clustered MaRBOH into four distinct subgroups (I, II, III and IV). The expression of MaRbohs following 24 hours of inoculation with Fusarium oxysporium f. sp. cubense Tropical
Race 4 revealed diverse patterns in root tissues. MaRbohH1 and MaRbohA2 were strongly upregulated and downregulated, respectively in response to the root-dip inoculation. This is the first report on genome-wide characterization of Rboh genes from banana and their expression in response to the fungal pathogen TR4. This research provides a basis for exploration of the role of NADPH oxidase in banana defence against the root pathogen. |
| first_indexed | 2024-03-06T10:47:08Z |
| format | Article |
| id | upm.eprints-89116 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:47:08Z |
| publishDate | 2020 |
| publisher | Malaysian Society for Biochemistry and Molecular Biology |
| record_format | dspace |
| spelling | upm.eprints-891162021-10-07T04:04:26Z http://psasir.upm.edu.my/id/eprint/89116/ Genome-wide identification and expression analysis of banana RBOH genes in response to Fusarium oxysporum f. sp. cubense Tropical Race 4 Chai, Siew Ying Tahir, Nur Fathiah Abu Bakar, Nur Syazana Teo, Chee How Mohd Yusoff, Nur Fatihah Baharum, Nadiya Akmal Saidi, Noor Baity Respiratory burst oxidase homologs (RBOH) is the key enzyme responsible for the production of reactive oxygen species which act as important signal during plant responses to abiotic and biotic stresses. However, RBOH homologs have not been characterized in banana. In this study, we have identified twelve Rboh genes distributed on eight chromosomes of Musa acuminata subsp. malaccensis (DH Pahang) through a genome-wide analysis. MaRbohs exist as sibling paralogs with variable exon-intron structures and highly conserved functional domain. Phylogenetic analysis clustered MaRBOH into four distinct subgroups (I, II, III and IV). The expression of MaRbohs following 24 hours of inoculation with Fusarium oxysporium f. sp. cubense Tropical Race 4 revealed diverse patterns in root tissues. MaRbohH1 and MaRbohA2 were strongly upregulated and downregulated, respectively in response to the root-dip inoculation. This is the first report on genome-wide characterization of Rboh genes from banana and their expression in response to the fungal pathogen TR4. This research provides a basis for exploration of the role of NADPH oxidase in banana defence against the root pathogen. Malaysian Society for Biochemistry and Molecular Biology 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/89116/1/ABSTRACT.pdf Chai, Siew Ying and Tahir, Nur Fathiah and Abu Bakar, Nur Syazana and Teo, Chee How and Mohd Yusoff, Nur Fatihah and Baharum, Nadiya Akmal and Saidi, Noor Baity (2020) Genome-wide identification and expression analysis of banana RBOH genes in response to Fusarium oxysporum f. sp. cubense Tropical Race 4. Malaysian Journal of Biochemistry and Molecular Biology, 23 (2). 46 - 52. ISSN 1511-2616 https://msbmb2010.wixsite.com/mjbmb/august-2020 |
| spellingShingle | Chai, Siew Ying Tahir, Nur Fathiah Abu Bakar, Nur Syazana Teo, Chee How Mohd Yusoff, Nur Fatihah Baharum, Nadiya Akmal Saidi, Noor Baity Genome-wide identification and expression analysis of banana RBOH genes in response to Fusarium oxysporum f. sp. cubense Tropical Race 4 |
| title | Genome-wide identification and expression analysis of banana RBOH genes in response to Fusarium oxysporum f. sp. cubense Tropical Race 4 |
| title_full | Genome-wide identification and expression analysis of banana RBOH genes in response to Fusarium oxysporum f. sp. cubense Tropical Race 4 |
| title_fullStr | Genome-wide identification and expression analysis of banana RBOH genes in response to Fusarium oxysporum f. sp. cubense Tropical Race 4 |
| title_full_unstemmed | Genome-wide identification and expression analysis of banana RBOH genes in response to Fusarium oxysporum f. sp. cubense Tropical Race 4 |
| title_short | Genome-wide identification and expression analysis of banana RBOH genes in response to Fusarium oxysporum f. sp. cubense Tropical Race 4 |
| title_sort | genome wide identification and expression analysis of banana rboh genes in response to fusarium oxysporum f sp cubense tropical race 4 |
| url | http://psasir.upm.edu.my/id/eprint/89116/1/ABSTRACT.pdf |
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