Identification of antioxidant and antibacterial activities for the bioactive peptides generated from bitter beans (Parkia speciosa) via boiling and fermentation processes
The present study reports on the effects of boiling and fermentation processes on the antioxidant activity and antibacterial activity of the peptides generated from bitter beans. The protein content, peptide content, antioxidant activity and antibacterial activity were determined. The peptides were...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/89253/1/BEAN.pdf |
| _version_ | 1825936647902986240 |
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| author | Muhialdin, Belal J. Abdul Rani, Nur Fatin Meor Hussin, Anis Shobirin |
| author_facet | Muhialdin, Belal J. Abdul Rani, Nur Fatin Meor Hussin, Anis Shobirin |
| author_sort | Muhialdin, Belal J. |
| collection | UPM |
| description | The present study reports on the effects of boiling and fermentation processes on the antioxidant activity and antibacterial activity of the peptides generated from bitter beans. The protein content, peptide content, antioxidant activity and antibacterial activity were determined. The peptides were fractionated using size exclusion and the identified using LC-MS/MS in combination with PEAKS Studio software. The protein content for raw, boiled and fermented samples was 20, 15 and 13 g/100g DW and the peptide content was 0.3, 12.1 and 15.1 mg/g DW, respectively. Fermentation process increased the DPPH activity of bitter beans to 78.48 ± 3.16%. Fermentation process significantly increased the antibacterial activity of bitter beans towards Escherichia coli (45%), Salmonella Typhimurium (73%), Staphylococcus aureus (64%), and Listeria monocytogenes (57%). Out of the seven peptides identified in fraction 11, three peptides including EAKPSFYLK, PVNNNAWAYATNFVPGK and AIGIFVKPDTAV demonstrated antibacterial activity at different concentrations (1000, 500, 250 and 125 μg/mL). The DPPH activity was only observed for the peptides EAKPSFYLK (74%) and PVNNNAWAYATNFVPGK (87%). In conclusion, fermentation process increased the antioxidant and antibacterial activity of bitter beans due to the presence of low molecular weight peptides. The bioactive peptides are very promising ingredients to formulate functional foods and beverages. |
| first_indexed | 2024-03-06T10:47:28Z |
| format | Article |
| id | upm.eprints-89253 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:47:28Z |
| publishDate | 2020 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-892532021-09-03T08:55:36Z http://psasir.upm.edu.my/id/eprint/89253/ Identification of antioxidant and antibacterial activities for the bioactive peptides generated from bitter beans (Parkia speciosa) via boiling and fermentation processes Muhialdin, Belal J. Abdul Rani, Nur Fatin Meor Hussin, Anis Shobirin The present study reports on the effects of boiling and fermentation processes on the antioxidant activity and antibacterial activity of the peptides generated from bitter beans. The protein content, peptide content, antioxidant activity and antibacterial activity were determined. The peptides were fractionated using size exclusion and the identified using LC-MS/MS in combination with PEAKS Studio software. The protein content for raw, boiled and fermented samples was 20, 15 and 13 g/100g DW and the peptide content was 0.3, 12.1 and 15.1 mg/g DW, respectively. Fermentation process increased the DPPH activity of bitter beans to 78.48 ± 3.16%. Fermentation process significantly increased the antibacterial activity of bitter beans towards Escherichia coli (45%), Salmonella Typhimurium (73%), Staphylococcus aureus (64%), and Listeria monocytogenes (57%). Out of the seven peptides identified in fraction 11, three peptides including EAKPSFYLK, PVNNNAWAYATNFVPGK and AIGIFVKPDTAV demonstrated antibacterial activity at different concentrations (1000, 500, 250 and 125 μg/mL). The DPPH activity was only observed for the peptides EAKPSFYLK (74%) and PVNNNAWAYATNFVPGK (87%). In conclusion, fermentation process increased the antioxidant and antibacterial activity of bitter beans due to the presence of low molecular weight peptides. The bioactive peptides are very promising ingredients to formulate functional foods and beverages. Elsevier 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/89253/1/BEAN.pdf Muhialdin, Belal J. and Abdul Rani, Nur Fatin and Meor Hussin, Anis Shobirin (2020) Identification of antioxidant and antibacterial activities for the bioactive peptides generated from bitter beans (Parkia speciosa) via boiling and fermentation processes. LWT - Food Science and Technology, 131. art. no. 109776. pp. 1-7. ISSN 0023-6438; ESSN: 1096-1127 https://www.sciencedirect.com/science/article/abs/pii/S0023643820307659 10.1016/j.lwt.2020.109776 |
| spellingShingle | Muhialdin, Belal J. Abdul Rani, Nur Fatin Meor Hussin, Anis Shobirin Identification of antioxidant and antibacterial activities for the bioactive peptides generated from bitter beans (Parkia speciosa) via boiling and fermentation processes |
| title | Identification of antioxidant and antibacterial activities for the bioactive peptides generated from bitter beans (Parkia speciosa) via boiling and fermentation processes |
| title_full | Identification of antioxidant and antibacterial activities for the bioactive peptides generated from bitter beans (Parkia speciosa) via boiling and fermentation processes |
| title_fullStr | Identification of antioxidant and antibacterial activities for the bioactive peptides generated from bitter beans (Parkia speciosa) via boiling and fermentation processes |
| title_full_unstemmed | Identification of antioxidant and antibacterial activities for the bioactive peptides generated from bitter beans (Parkia speciosa) via boiling and fermentation processes |
| title_short | Identification of antioxidant and antibacterial activities for the bioactive peptides generated from bitter beans (Parkia speciosa) via boiling and fermentation processes |
| title_sort | identification of antioxidant and antibacterial activities for the bioactive peptides generated from bitter beans parkia speciosa via boiling and fermentation processes |
| url | http://psasir.upm.edu.my/id/eprint/89253/1/BEAN.pdf |
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