Immunological analysis of the Hepatitis B virus “a” determinant displayed on chimeric virus-like particles of Macrobrachium rosenbergii nodavirus capsid protein produced in Sf9 cells
Chimeric virus-like particles (VLPs) have been widely exploited for various purposes including their use as vaccine candidates, particularly due to their ability to induce stronger immune responses than VLPs consisting of single viral proteins. In the present study, VLPs of the Macrobrachium rosenbe...
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| Format: | Article |
| Language: | English |
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Multidisciplinary Digital Publishing Institute
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/89272/1/MICE.pdf |
| _version_ | 1825936651601313792 |
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| author | Ninyio, Nathaniel Nyakaat Ho, Kok Lian Ong, Hui Kian Yong, Chean Yeah Chee, Hui Yee Hamid, Muhajir Tan, Wen Siang |
| author_facet | Ninyio, Nathaniel Nyakaat Ho, Kok Lian Ong, Hui Kian Yong, Chean Yeah Chee, Hui Yee Hamid, Muhajir Tan, Wen Siang |
| author_sort | Ninyio, Nathaniel Nyakaat |
| collection | UPM |
| description | Chimeric virus-like particles (VLPs) have been widely exploited for various purposes including their use as vaccine candidates, particularly due to their ability to induce stronger immune responses than VLPs consisting of single viral proteins. In the present study, VLPs of the Macrobrachium rosenbergii nodavirus (MrNV) capsid protein (Nc) displaying the hepatitis B virus “a” determinant (aD) were produced in Spodoptera frugiperda (Sf9) insect cells. BALB/c mice immunised with the purified chimeric Nc-aD VLPs elicited a sustained titre of anti-aD antibody, which was significantly higher than that elicited by a commercially available hepatitis B vaccine and Escherichia coli-produced Nc-aD VLPs. Immunophenotyping showed that the Sf9-produced Nc-aD VLPs induced proliferation of cytotoxic T-lymphocytes and NK1.1 natural killer cells. Furthermore, enzyme-linked immunospot (ELISPOT)analysis showed the presence of antibody-secreting memory B cells in the mice splenocytes stimulated with the synthetic aD peptide. The significant humoral, natural killer cell and memory B cell immune responses induced by the Sf9-produced Nc-aD VLPs suggest that they present good prospects for use as a hepatitis B vaccine candidate. |
| first_indexed | 2024-03-06T10:47:32Z |
| format | Article |
| id | upm.eprints-89272 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:47:32Z |
| publishDate | 2020 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | upm.eprints-892722021-09-20T22:58:56Z http://psasir.upm.edu.my/id/eprint/89272/ Immunological analysis of the Hepatitis B virus “a” determinant displayed on chimeric virus-like particles of Macrobrachium rosenbergii nodavirus capsid protein produced in Sf9 cells Ninyio, Nathaniel Nyakaat Ho, Kok Lian Ong, Hui Kian Yong, Chean Yeah Chee, Hui Yee Hamid, Muhajir Tan, Wen Siang Chimeric virus-like particles (VLPs) have been widely exploited for various purposes including their use as vaccine candidates, particularly due to their ability to induce stronger immune responses than VLPs consisting of single viral proteins. In the present study, VLPs of the Macrobrachium rosenbergii nodavirus (MrNV) capsid protein (Nc) displaying the hepatitis B virus “a” determinant (aD) were produced in Spodoptera frugiperda (Sf9) insect cells. BALB/c mice immunised with the purified chimeric Nc-aD VLPs elicited a sustained titre of anti-aD antibody, which was significantly higher than that elicited by a commercially available hepatitis B vaccine and Escherichia coli-produced Nc-aD VLPs. Immunophenotyping showed that the Sf9-produced Nc-aD VLPs induced proliferation of cytotoxic T-lymphocytes and NK1.1 natural killer cells. Furthermore, enzyme-linked immunospot (ELISPOT)analysis showed the presence of antibody-secreting memory B cells in the mice splenocytes stimulated with the synthetic aD peptide. The significant humoral, natural killer cell and memory B cell immune responses induced by the Sf9-produced Nc-aD VLPs suggest that they present good prospects for use as a hepatitis B vaccine candidate. Multidisciplinary Digital Publishing Institute 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/89272/1/MICE.pdf Ninyio, Nathaniel Nyakaat and Ho, Kok Lian and Ong, Hui Kian and Yong, Chean Yeah and Chee, Hui Yee and Hamid, Muhajir and Tan, Wen Siang (2020) Immunological analysis of the Hepatitis B virus “a” determinant displayed on chimeric virus-like particles of Macrobrachium rosenbergii nodavirus capsid protein produced in Sf9 cells. Vaccines, 8 (2). art. no. 275. pp. 1-15. ISSN 2076-393X https://www.mdpi.com/2076-393X/8/2/275 10.3390/vaccines8020275 |
| spellingShingle | Ninyio, Nathaniel Nyakaat Ho, Kok Lian Ong, Hui Kian Yong, Chean Yeah Chee, Hui Yee Hamid, Muhajir Tan, Wen Siang Immunological analysis of the Hepatitis B virus “a” determinant displayed on chimeric virus-like particles of Macrobrachium rosenbergii nodavirus capsid protein produced in Sf9 cells |
| title | Immunological analysis of the Hepatitis B virus “a” determinant displayed on chimeric virus-like particles of Macrobrachium rosenbergii nodavirus capsid protein produced in Sf9 cells |
| title_full | Immunological analysis of the Hepatitis B virus “a” determinant displayed on chimeric virus-like particles of Macrobrachium rosenbergii nodavirus capsid protein produced in Sf9 cells |
| title_fullStr | Immunological analysis of the Hepatitis B virus “a” determinant displayed on chimeric virus-like particles of Macrobrachium rosenbergii nodavirus capsid protein produced in Sf9 cells |
| title_full_unstemmed | Immunological analysis of the Hepatitis B virus “a” determinant displayed on chimeric virus-like particles of Macrobrachium rosenbergii nodavirus capsid protein produced in Sf9 cells |
| title_short | Immunological analysis of the Hepatitis B virus “a” determinant displayed on chimeric virus-like particles of Macrobrachium rosenbergii nodavirus capsid protein produced in Sf9 cells |
| title_sort | immunological analysis of the hepatitis b virus a determinant displayed on chimeric virus like particles of macrobrachium rosenbergii nodavirus capsid protein produced in sf9 cells |
| url | http://psasir.upm.edu.my/id/eprint/89272/1/MICE.pdf |
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