Lavender essential oil induces oxidative stress which modifies teh bacterial membrane permeability of carbapenase producing Klebsiella pnuemoniae
Misuse of antibiotics in the clinical and agricultural sectors has caused the emergence of multidrugresistant (MDR) Klebsiella pneumoniae which contributes a threat to human health. In this study, we assessed the feasibility of lavender essential oil (LVO) as an antimicrobial agent in combinatory th...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/89556/1/ABSTRACT.pdf |
| _version_ | 1825936697704054784 |
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| author | Yang, Shun Kai Yusoff, Khatijah Thomas, Warren Akseer, Riaz Sultan Alhosani, Maryam Abushelaibi, Aisha Lim, Swee Hua Erin Lai, Kok Song |
| author_facet | Yang, Shun Kai Yusoff, Khatijah Thomas, Warren Akseer, Riaz Sultan Alhosani, Maryam Abushelaibi, Aisha Lim, Swee Hua Erin Lai, Kok Song |
| author_sort | Yang, Shun Kai |
| collection | UPM |
| description | Misuse of antibiotics in the clinical and agricultural sectors has caused the emergence of multidrugresistant (MDR) Klebsiella pneumoniae which contributes a threat to human health. In this study, we assessed the feasibility of lavender essential oil (LVO) as an antimicrobial agent in combinatory therapy with meropenem in suppressing the growth of carbapenemase-producing K. pneumoniae (KPC-KP). Synergistic interactions between LVO and meropenem were detected, which signifcantly reduce the
inhibitory concentration of both LVO and meropenem by 15 and 4-fold respectively. Comparative proteomic profling identifed a disruption in the bacterial membrane via oxidative stress that was
indicated by loss of membrane and cytoplasmic proteins and the upregulation of oxidative regulators. As a proof of concept, zeta potential measurements showed a change in cell surface charge while outer membrane permeability measurement indicated an increase in membrane permeability following exposure to LVO. This was indicative of a disrupted outer membrane. Ethidium bromide infux/efux assays demonstrated no signifcant efux pump inhibition by LVO, and scanning electron microscopy revealed irregularities on the cell surface after exposure to LVO. Oxidative stress was also detected with increased level of ROS and lipid peroxidation in LVO-treated cells. In conclusion, our data suggest that LVO induced oxidative stress in K. pneumoniae which oxidizes the outer membrane, enabling the infux of generated ROS, LVO and meropenem into the bacterial cells, causing damage to the cells and eventually death. |
| first_indexed | 2024-03-06T10:48:14Z |
| format | Article |
| id | upm.eprints-89556 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:48:14Z |
| publishDate | 2020 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | upm.eprints-895562021-11-01T13:48:31Z http://psasir.upm.edu.my/id/eprint/89556/ Lavender essential oil induces oxidative stress which modifies teh bacterial membrane permeability of carbapenase producing Klebsiella pnuemoniae Yang, Shun Kai Yusoff, Khatijah Thomas, Warren Akseer, Riaz Sultan Alhosani, Maryam Abushelaibi, Aisha Lim, Swee Hua Erin Lai, Kok Song Misuse of antibiotics in the clinical and agricultural sectors has caused the emergence of multidrugresistant (MDR) Klebsiella pneumoniae which contributes a threat to human health. In this study, we assessed the feasibility of lavender essential oil (LVO) as an antimicrobial agent in combinatory therapy with meropenem in suppressing the growth of carbapenemase-producing K. pneumoniae (KPC-KP). Synergistic interactions between LVO and meropenem were detected, which signifcantly reduce the inhibitory concentration of both LVO and meropenem by 15 and 4-fold respectively. Comparative proteomic profling identifed a disruption in the bacterial membrane via oxidative stress that was indicated by loss of membrane and cytoplasmic proteins and the upregulation of oxidative regulators. As a proof of concept, zeta potential measurements showed a change in cell surface charge while outer membrane permeability measurement indicated an increase in membrane permeability following exposure to LVO. This was indicative of a disrupted outer membrane. Ethidium bromide infux/efux assays demonstrated no signifcant efux pump inhibition by LVO, and scanning electron microscopy revealed irregularities on the cell surface after exposure to LVO. Oxidative stress was also detected with increased level of ROS and lipid peroxidation in LVO-treated cells. In conclusion, our data suggest that LVO induced oxidative stress in K. pneumoniae which oxidizes the outer membrane, enabling the infux of generated ROS, LVO and meropenem into the bacterial cells, causing damage to the cells and eventually death. Nature Publishing Group 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/89556/1/ABSTRACT.pdf Yang, Shun Kai and Yusoff, Khatijah and Thomas, Warren and Akseer, Riaz and Sultan Alhosani, Maryam and Abushelaibi, Aisha and Lim, Swee Hua Erin and Lai, Kok Song (2020) Lavender essential oil induces oxidative stress which modifies teh bacterial membrane permeability of carbapenase producing Klebsiella pnuemoniae. Scientific Reports, 10 (1). art. no. 819. pp. 1-14. ISSN 2045-2322 https://www.nature.com/articles/s41598-019-55601-0 10.1038/s41598-019-55601-0 |
| spellingShingle | Yang, Shun Kai Yusoff, Khatijah Thomas, Warren Akseer, Riaz Sultan Alhosani, Maryam Abushelaibi, Aisha Lim, Swee Hua Erin Lai, Kok Song Lavender essential oil induces oxidative stress which modifies teh bacterial membrane permeability of carbapenase producing Klebsiella pnuemoniae |
| title | Lavender essential oil induces oxidative stress which modifies teh bacterial membrane permeability of carbapenase producing Klebsiella pnuemoniae |
| title_full | Lavender essential oil induces oxidative stress which modifies teh bacterial membrane permeability of carbapenase producing Klebsiella pnuemoniae |
| title_fullStr | Lavender essential oil induces oxidative stress which modifies teh bacterial membrane permeability of carbapenase producing Klebsiella pnuemoniae |
| title_full_unstemmed | Lavender essential oil induces oxidative stress which modifies teh bacterial membrane permeability of carbapenase producing Klebsiella pnuemoniae |
| title_short | Lavender essential oil induces oxidative stress which modifies teh bacterial membrane permeability of carbapenase producing Klebsiella pnuemoniae |
| title_sort | lavender essential oil induces oxidative stress which modifies teh bacterial membrane permeability of carbapenase producing klebsiella pnuemoniae |
| url | http://psasir.upm.edu.my/id/eprint/89556/1/ABSTRACT.pdf |
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