Comparison of the growth of two Arabidopsis thaliana ecotypes and tranformation efficiency in UPM’s transgenic house
Arabidopsis thaliana is a small flowering plant in the mustard family (Brassicaceae). It is used to study the genetic improvement of plants and is a model organism in agricultural biotechnology. Therefore, the identification of suitable varieties for genetic experiments including the Columbia (Co...
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| Format: | Project Paper Report |
| Language: | English |
| Published: |
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/90141/1/FP%202015%20144%20UPM%20IR.pdf |
| Summary: | Arabidopsis thaliana is a small flowering plant in the mustard family (Brassicaceae). It
is used to study the genetic improvement of plants and is a model organism in
agricultural biotechnology. Therefore, the identification of suitable varieties for genetic
experiments including the Columbia (Col-O) ecotype and Landsberg (Ler-0) ecotype is
important. The objective of this experiment is to compare the growth rate of Columbia
(Col-O) and Landsberg (Ler-0) A. thaliana ecotypes and the transformation efficiency
in UPM's Transgenic Green House. The parameters used to measure the growth rate
were size of leaf and height of plant. The Columbia (Col-O) and Landsberg (Ler-0) took
around eight weeks two finish the life cycle. The Columbia (Col-O) has been reported
to have very robust growth and average growth characteristic compared to Landsberg
(Ler-0) which has shorter stems and smaller siliques. It is expected that the two
ecotypes will also vary in the growth rate and transformation efficiency when grown in
the UPM’s Transgenic Greenhouse. Agrobacterium tumefaciens strain LBA4404 that
harbour the binary vector pMDC32 will mediate transformation of A. thaliana which
was performed on the Columbia (Col-0) ecotypes and the transformation efficiency was
compared subsequently after culturing on regeneration medium with containing
hygromycin at 60 mg/litre. The transformation was successfully where the percentages
seeds germinated on MS media are 56.67% with the addition of the antibiotic. |
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