Fibronectin-binding protein F1 (prtF1) gene is highly distributed in tetracycline-resistant, erythromycin-sensitive streptococcus pyogenes isolates
Introduction: The internalization process of group A streptococci (GAS) into human cells is one of the crucial steps in the pathogenesis of GAS infections, which could also affect their susceptibility responses toward several antibiotics. Currently, data on the distribution of internalization-associ...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/90356/1/2021040613492033_MJMHS_0821.pdf |
| _version_ | 1825936815922610176 |
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| author | Simon, Onyema Azi Kumar, Suresh Than, Leslie Thian Lung Osman, Malina Awang Hamat, Rukman |
| author_facet | Simon, Onyema Azi Kumar, Suresh Than, Leslie Thian Lung Osman, Malina Awang Hamat, Rukman |
| author_sort | Simon, Onyema Azi |
| collection | UPM |
| description | Introduction: The internalization process of group A streptococci (GAS) into human cells is one of the crucial steps in the pathogenesis of GAS infections, which could also affect their susceptibility responses toward several antibiotics. Currently, data on the distribution of internalization-associated genes and susceptibility patterns are still lacking in Malaysia. This study investigated the distribution of fibronectin-binding protein F1 (prtF1) and streptococcal pyrogenic exotoxin B (speB) genes in GAS isolates with their susceptibility profiles and source of samples. Methods: We used 43 GAS isolates from our previous stock culture and performed antibiotic susceptibility testing by Kirby-Bauer disk diffusion method and interpreted the results according to the established guidelines. We detected virulence (prtF1 and speB) and resistance (ermA, ermB, mefA, tetM and lnuA) genes by PCR method using established primers and protocols. Results: High resistance rates were observed against doxycycline (58.1%) and clindamycin (16.3%). In comparison, 100.0% and 46.5% of GAS isolates carried speB and prtF1 genes, respectively. tetM and lnuA genes were detected in all respective resistant isolates (100% for each). No macrolide resistance genes were detected. Interestingly, prtF1 gene was highly distributed in doxycycline-resistant than doxycycline-sensitive isolates (60.0% versus 27.8%). Conclusions: High resistance rate of GAS toward doxycycline in our study may potentially reflect the uncontrol dissemination of tetM gene among our isolates. The presence of prtF1 gene among this strain would enhance its ability to evade the intracellular action of antibiotics, which may affect the management of GAS diseases. Thus, close monitoring of GAS by molecular methods is required in the future. |
| first_indexed | 2024-03-06T10:50:02Z |
| format | Article |
| id | upm.eprints-90356 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:50:02Z |
| publishDate | 2021 |
| publisher | Faculty of Medicine and Health Sciences, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-903562021-09-10T00:59:07Z http://psasir.upm.edu.my/id/eprint/90356/ Fibronectin-binding protein F1 (prtF1) gene is highly distributed in tetracycline-resistant, erythromycin-sensitive streptococcus pyogenes isolates Simon, Onyema Azi Kumar, Suresh Than, Leslie Thian Lung Osman, Malina Awang Hamat, Rukman Introduction: The internalization process of group A streptococci (GAS) into human cells is one of the crucial steps in the pathogenesis of GAS infections, which could also affect their susceptibility responses toward several antibiotics. Currently, data on the distribution of internalization-associated genes and susceptibility patterns are still lacking in Malaysia. This study investigated the distribution of fibronectin-binding protein F1 (prtF1) and streptococcal pyrogenic exotoxin B (speB) genes in GAS isolates with their susceptibility profiles and source of samples. Methods: We used 43 GAS isolates from our previous stock culture and performed antibiotic susceptibility testing by Kirby-Bauer disk diffusion method and interpreted the results according to the established guidelines. We detected virulence (prtF1 and speB) and resistance (ermA, ermB, mefA, tetM and lnuA) genes by PCR method using established primers and protocols. Results: High resistance rates were observed against doxycycline (58.1%) and clindamycin (16.3%). In comparison, 100.0% and 46.5% of GAS isolates carried speB and prtF1 genes, respectively. tetM and lnuA genes were detected in all respective resistant isolates (100% for each). No macrolide resistance genes were detected. Interestingly, prtF1 gene was highly distributed in doxycycline-resistant than doxycycline-sensitive isolates (60.0% versus 27.8%). Conclusions: High resistance rate of GAS toward doxycycline in our study may potentially reflect the uncontrol dissemination of tetM gene among our isolates. The presence of prtF1 gene among this strain would enhance its ability to evade the intracellular action of antibiotics, which may affect the management of GAS diseases. Thus, close monitoring of GAS by molecular methods is required in the future. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2021-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/90356/1/2021040613492033_MJMHS_0821.pdf Simon, Onyema Azi and Kumar, Suresh and Than, Leslie Thian Lung and Osman, Malina and Awang Hamat, Rukman (2021) Fibronectin-binding protein F1 (prtF1) gene is highly distributed in tetracycline-resistant, erythromycin-sensitive streptococcus pyogenes isolates. Malaysian Journal of Medicine and Health Sciences, 17 (2). pp. 239-245. ISSN 1675-8544; ESSN: 2636-9346 https://medic.upm.edu.my/upload/dokumen/2021040613492033_MJMHS_0821.pdf |
| spellingShingle | Simon, Onyema Azi Kumar, Suresh Than, Leslie Thian Lung Osman, Malina Awang Hamat, Rukman Fibronectin-binding protein F1 (prtF1) gene is highly distributed in tetracycline-resistant, erythromycin-sensitive streptococcus pyogenes isolates |
| title | Fibronectin-binding protein F1 (prtF1) gene is highly distributed in tetracycline-resistant, erythromycin-sensitive streptococcus pyogenes isolates |
| title_full | Fibronectin-binding protein F1 (prtF1) gene is highly distributed in tetracycline-resistant, erythromycin-sensitive streptococcus pyogenes isolates |
| title_fullStr | Fibronectin-binding protein F1 (prtF1) gene is highly distributed in tetracycline-resistant, erythromycin-sensitive streptococcus pyogenes isolates |
| title_full_unstemmed | Fibronectin-binding protein F1 (prtF1) gene is highly distributed in tetracycline-resistant, erythromycin-sensitive streptococcus pyogenes isolates |
| title_short | Fibronectin-binding protein F1 (prtF1) gene is highly distributed in tetracycline-resistant, erythromycin-sensitive streptococcus pyogenes isolates |
| title_sort | fibronectin binding protein f1 prtf1 gene is highly distributed in tetracycline resistant erythromycin sensitive streptococcus pyogenes isolates |
| url | http://psasir.upm.edu.my/id/eprint/90356/1/2021040613492033_MJMHS_0821.pdf |
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