In vitro catharanthus roseus (L.) G. don callus responses to chitosan treatments

The present study investigates the effects of chitosan on callus growth and development ofCatharanthusroseus under light treatment. In the study, chitosan was supplemented in culture medium. Callus of C. roseus, previously initiated on MS medium before the onset of the experiment was cultured. Vario...

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Main Author: Ismail, Nur Atikah
Format: Project Paper Report
Language:English
Published: 2015
Online Access:http://psasir.upm.edu.my/id/eprint/91590/1/FP%202015%2089%20-%20IR.pdf
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author Ismail, Nur Atikah
author_facet Ismail, Nur Atikah
author_sort Ismail, Nur Atikah
collection UPM
description The present study investigates the effects of chitosan on callus growth and development ofCatharanthusroseus under light treatment. In the study, chitosan was supplemented in culture medium. Callus of C. roseus, previously initiated on MS medium before the onset of the experiment was cultured. Various levels of chitosan (0, 1, 2, 3, 4, 5 mg/L) were used. The experiment was conducted in a completely randomized design with 3 replications. Study parameters include dry weight of callus andboron level. Data recorded that the control, 0 mg/L chitosan concentration gave the highest dry weight of callus 0.210 g. Treatment with 1 mg/L chitosan concentration increased boron level in callus of C. roseusto 0.097 mg/L. The study suggests that the application of chitosan in culture medium did not give significant increase in dry weight of callus compared to the control. Chitosan treatment did not give significant increase or decrease in boron level of callus. The study concludes that chitosan had no significant effect on dry weight and boron level in callus of C. roseus under light treatment.
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spelling upm.eprints-915902021-12-01T04:05:39Z http://psasir.upm.edu.my/id/eprint/91590/ In vitro catharanthus roseus (L.) G. don callus responses to chitosan treatments Ismail, Nur Atikah The present study investigates the effects of chitosan on callus growth and development ofCatharanthusroseus under light treatment. In the study, chitosan was supplemented in culture medium. Callus of C. roseus, previously initiated on MS medium before the onset of the experiment was cultured. Various levels of chitosan (0, 1, 2, 3, 4, 5 mg/L) were used. The experiment was conducted in a completely randomized design with 3 replications. Study parameters include dry weight of callus andboron level. Data recorded that the control, 0 mg/L chitosan concentration gave the highest dry weight of callus 0.210 g. Treatment with 1 mg/L chitosan concentration increased boron level in callus of C. roseusto 0.097 mg/L. The study suggests that the application of chitosan in culture medium did not give significant increase in dry weight of callus compared to the control. Chitosan treatment did not give significant increase or decrease in boron level of callus. The study concludes that chitosan had no significant effect on dry weight and boron level in callus of C. roseus under light treatment. 2015 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/91590/1/FP%202015%2089%20-%20IR.pdf Ismail, Nur Atikah (2015) In vitro catharanthus roseus (L.) G. don callus responses to chitosan treatments. [Project Paper Report]
spellingShingle Ismail, Nur Atikah
In vitro catharanthus roseus (L.) G. don callus responses to chitosan treatments
title In vitro catharanthus roseus (L.) G. don callus responses to chitosan treatments
title_full In vitro catharanthus roseus (L.) G. don callus responses to chitosan treatments
title_fullStr In vitro catharanthus roseus (L.) G. don callus responses to chitosan treatments
title_full_unstemmed In vitro catharanthus roseus (L.) G. don callus responses to chitosan treatments
title_short In vitro catharanthus roseus (L.) G. don callus responses to chitosan treatments
title_sort in vitro catharanthus roseus l g don callus responses to chitosan treatments
url http://psasir.upm.edu.my/id/eprint/91590/1/FP%202015%2089%20-%20IR.pdf
work_keys_str_mv AT ismailnuratikah invitrocatharanthusroseuslgdoncallusresponsestochitosantreatments