The wheat Sr22, Sr33, Sr35 and Sr45 genes confer resistance against stem rust in barley
In the last 20 years, stem rust caused by the fungus Puccinia graminis f. sp. tritici (Pgt), has re-emerged as a major threat to wheat and barley production in Africa and Europe. In contrast to wheat with 60 designated stem rust (Sr) resistance genes, barley’s genetic variation for stem rust resista...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/93367/1/The%20wheat%20Sr22.pdf |
| _version_ | 1825937349840732160 |
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| author | Md Hatta, Muhammad Asyraf Arora, Sanu Ghosh, Sreya Matny, Oadi Smedley, Mark A. Yu, Guotai Chakraborty, Soma Bhatt, Dhara Xia, Xiaodi Steuernagel, Burkhard Richardson, Terese Mago, Rohit Lagudah, Evans S. Patron, Nicola Ayliffe, Michael Rouse, Matthew N. Harwood, Wendy Periyannan, Sambasivam |
| author_facet | Md Hatta, Muhammad Asyraf Arora, Sanu Ghosh, Sreya Matny, Oadi Smedley, Mark A. Yu, Guotai Chakraborty, Soma Bhatt, Dhara Xia, Xiaodi Steuernagel, Burkhard Richardson, Terese Mago, Rohit Lagudah, Evans S. Patron, Nicola Ayliffe, Michael Rouse, Matthew N. Harwood, Wendy Periyannan, Sambasivam |
| author_sort | Md Hatta, Muhammad Asyraf |
| collection | UPM |
| description | In the last 20 years, stem rust caused by the fungus Puccinia graminis f. sp. tritici (Pgt), has re-emerged as a major threat to wheat and barley production in Africa and Europe. In contrast to wheat with 60 designated stem rust (Sr) resistance genes, barley’s genetic variation for stem rust resistance is very narrow with only ten resistance genes genetically identified. Of these, only one complex locus consisting of three genes is effective against TTKSK, a widely virulent Pgt race of the Ug99 tribe which emerged in Uganda in 1999 and has since spread to much of East Africa and parts of the Middle East. The objective of this study was to assess the functionality, in barley, of cloned wheat Sr genes effective against race TTKSK. Sr22, Sr33, Sr35 and Sr45 were transformed into barley cv. Golden Promise using Agrobacterium-mediated transformation. All four genes were found to confer effective stem rust resistance. The barley transgenics remained susceptible to the barley leaf rust pathogen Puccinia hordei, indicating that the resistance conferred by these wheat Sr genes was specific for Pgt. Furthermore, these transgenic plants did not display significant adverse agronomic effects in the absence of disease. Cloned Sr genes from wheat are therefore a potential source of resistance against wheat stem rust in barley. |
| first_indexed | 2024-03-06T10:58:11Z |
| format | Article |
| id | upm.eprints-93367 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:58:11Z |
| publishDate | 2021 |
| publisher | Wiley |
| record_format | dspace |
| spelling | upm.eprints-933672022-11-22T07:24:44Z http://psasir.upm.edu.my/id/eprint/93367/ The wheat Sr22, Sr33, Sr35 and Sr45 genes confer resistance against stem rust in barley Md Hatta, Muhammad Asyraf Arora, Sanu Ghosh, Sreya Matny, Oadi Smedley, Mark A. Yu, Guotai Chakraborty, Soma Bhatt, Dhara Xia, Xiaodi Steuernagel, Burkhard Richardson, Terese Mago, Rohit Lagudah, Evans S. Patron, Nicola Ayliffe, Michael Rouse, Matthew N. Harwood, Wendy Periyannan, Sambasivam In the last 20 years, stem rust caused by the fungus Puccinia graminis f. sp. tritici (Pgt), has re-emerged as a major threat to wheat and barley production in Africa and Europe. In contrast to wheat with 60 designated stem rust (Sr) resistance genes, barley’s genetic variation for stem rust resistance is very narrow with only ten resistance genes genetically identified. Of these, only one complex locus consisting of three genes is effective against TTKSK, a widely virulent Pgt race of the Ug99 tribe which emerged in Uganda in 1999 and has since spread to much of East Africa and parts of the Middle East. The objective of this study was to assess the functionality, in barley, of cloned wheat Sr genes effective against race TTKSK. Sr22, Sr33, Sr35 and Sr45 were transformed into barley cv. Golden Promise using Agrobacterium-mediated transformation. All four genes were found to confer effective stem rust resistance. The barley transgenics remained susceptible to the barley leaf rust pathogen Puccinia hordei, indicating that the resistance conferred by these wheat Sr genes was specific for Pgt. Furthermore, these transgenic plants did not display significant adverse agronomic effects in the absence of disease. Cloned Sr genes from wheat are therefore a potential source of resistance against wheat stem rust in barley. Wiley 2021-02 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/93367/1/The%20wheat%20Sr22.pdf Md Hatta, Muhammad Asyraf and Arora, Sanu and Ghosh, Sreya and Matny, Oadi and Smedley, Mark A. and Yu, Guotai and Chakraborty, Soma and Bhatt, Dhara and Xia, Xiaodi and Steuernagel, Burkhard and Richardson, Terese and Mago, Rohit and Lagudah, Evans S. and Patron, Nicola and Ayliffe, Michael and Rouse, Matthew N. and Harwood, Wendy and Periyannan, Sambasivam (2021) The wheat Sr22, Sr33, Sr35 and Sr45 genes confer resistance against stem rust in barley. Plant Biotechnology Journal, 19 (2). 273 - 284. ISSN 1467-7644; ESSN:1467-7652 https://onlinelibrary.wiley.com/doi/full/10.1111/pbi.13460 10.1111/pbi.13460 |
| spellingShingle | Md Hatta, Muhammad Asyraf Arora, Sanu Ghosh, Sreya Matny, Oadi Smedley, Mark A. Yu, Guotai Chakraborty, Soma Bhatt, Dhara Xia, Xiaodi Steuernagel, Burkhard Richardson, Terese Mago, Rohit Lagudah, Evans S. Patron, Nicola Ayliffe, Michael Rouse, Matthew N. Harwood, Wendy Periyannan, Sambasivam The wheat Sr22, Sr33, Sr35 and Sr45 genes confer resistance against stem rust in barley |
| title | The wheat Sr22, Sr33, Sr35 and Sr45 genes confer resistance against stem rust in barley |
| title_full | The wheat Sr22, Sr33, Sr35 and Sr45 genes confer resistance against stem rust in barley |
| title_fullStr | The wheat Sr22, Sr33, Sr35 and Sr45 genes confer resistance against stem rust in barley |
| title_full_unstemmed | The wheat Sr22, Sr33, Sr35 and Sr45 genes confer resistance against stem rust in barley |
| title_short | The wheat Sr22, Sr33, Sr35 and Sr45 genes confer resistance against stem rust in barley |
| title_sort | wheat sr22 sr33 sr35 and sr45 genes confer resistance against stem rust in barley |
| url | http://psasir.upm.edu.my/id/eprint/93367/1/The%20wheat%20Sr22.pdf |
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