Upregulation and hypomethylation of EGFR in Formalin-fixed Paraffin Embedded FFPE tissues of colon adenocarcinoma
Introduction: Colorectal cancer (CRC) is the third most common cancer worldwide. Even though many cancer therapies have been developed, considerable proportions of patients respond poorly to therapy and the number of resistance cases increases. CRC emerges as a result of genetic and/or epigenetic m...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Faculty of Medicine and Health Science, Universiti Putra Malaysia
2021
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/93494/1/2021120708131401_MJMHS_0571.pdf |
| _version_ | 1796982888808316928 |
|---|---|
| author | Othman, Rosfayati Mohtaruddin, Norhafizah Ahmad Zubir, Nurul Mahirah Seow, Heng Fong Ngan, Kah Wai Osman, Malina |
| author_facet | Othman, Rosfayati Mohtaruddin, Norhafizah Ahmad Zubir, Nurul Mahirah Seow, Heng Fong Ngan, Kah Wai Osman, Malina |
| author_sort | Othman, Rosfayati |
| collection | UPM |
| description | Introduction: Colorectal cancer (CRC) is the third most common cancer worldwide. Even though many cancer therapies have been developed, considerable proportions of patients respond poorly to therapy and the number of resistance cases increases. CRC emerges as a result of genetic and/or epigenetic modifications of epidermal growth factor receptor (EGFR) in colonic epithelial cells during tumourigenesis. Determination of DNA methylation status of EGFR is very crucial to further understand the role of this gene in carcinogenesis. However, the applicability of formalin-fixed paraffin embedded (FFPE) tissues in molecular studies is still limited due to high degradation of the nucleic acids. Hence, this study aimed to determine the gene expression and DNA methylation status of EGFR in FFPE CRC samples. Methods: Fifty-nine of archival FFPE CRC cases with the adjacent normal colon tissues were retrieved. Manual micro-dissection was performed prior to RNA and DNA extraction. EGFR expression and DNA methylation status was evaluated by qPCR and methylation specific PCR (MSP) techniques respectively. Results: EGFR was overexpressed in 54.2% (p-value=0.021) of CRC cases. Hypomethylation of EGFR was discovered in 81.4% and 79.7% of FFPE CRC tissues and normal adjacent tissues respectively. No significant association was found between DNA methylation and mRNA levels of EGFR. Conclusion: Determination of gene expression and DNA methylation in FFPE tissues were successfully carried out. The overexpression and hypomethylation of EGFR strongly suggest its important role in CRC tumourigenesis. Hypomethylation in normal tissue adjacent to the tumours indicates this epigenetic change occurs at the early step in carcinogenesis. |
| first_indexed | 2024-03-06T10:58:28Z |
| format | Article |
| id | upm.eprints-93494 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:58:28Z |
| publishDate | 2021 |
| publisher | Faculty of Medicine and Health Science, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-934942023-10-20T02:51:51Z http://psasir.upm.edu.my/id/eprint/93494/ Upregulation and hypomethylation of EGFR in Formalin-fixed Paraffin Embedded FFPE tissues of colon adenocarcinoma Othman, Rosfayati Mohtaruddin, Norhafizah Ahmad Zubir, Nurul Mahirah Seow, Heng Fong Ngan, Kah Wai Osman, Malina Introduction: Colorectal cancer (CRC) is the third most common cancer worldwide. Even though many cancer therapies have been developed, considerable proportions of patients respond poorly to therapy and the number of resistance cases increases. CRC emerges as a result of genetic and/or epigenetic modifications of epidermal growth factor receptor (EGFR) in colonic epithelial cells during tumourigenesis. Determination of DNA methylation status of EGFR is very crucial to further understand the role of this gene in carcinogenesis. However, the applicability of formalin-fixed paraffin embedded (FFPE) tissues in molecular studies is still limited due to high degradation of the nucleic acids. Hence, this study aimed to determine the gene expression and DNA methylation status of EGFR in FFPE CRC samples. Methods: Fifty-nine of archival FFPE CRC cases with the adjacent normal colon tissues were retrieved. Manual micro-dissection was performed prior to RNA and DNA extraction. EGFR expression and DNA methylation status was evaluated by qPCR and methylation specific PCR (MSP) techniques respectively. Results: EGFR was overexpressed in 54.2% (p-value=0.021) of CRC cases. Hypomethylation of EGFR was discovered in 81.4% and 79.7% of FFPE CRC tissues and normal adjacent tissues respectively. No significant association was found between DNA methylation and mRNA levels of EGFR. Conclusion: Determination of gene expression and DNA methylation in FFPE tissues were successfully carried out. The overexpression and hypomethylation of EGFR strongly suggest its important role in CRC tumourigenesis. Hypomethylation in normal tissue adjacent to the tumours indicates this epigenetic change occurs at the early step in carcinogenesis. Faculty of Medicine and Health Science, Universiti Putra Malaysia 2021-12 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/93494/1/2021120708131401_MJMHS_0571.pdf Othman, Rosfayati and Mohtaruddin, Norhafizah and Ahmad Zubir, Nurul Mahirah and Seow, Heng Fong and Ngan, Kah Wai and Osman, Malina (2021) Upregulation and hypomethylation of EGFR in Formalin-fixed Paraffin Embedded FFPE tissues of colon adenocarcinoma. Malaysian Journal of Medicine and Health Sciences, 17 (supp.10). pp. 2-9. ISSN 2636-9346 https://medic.upm.edu.my/jurnal_kami/volume_17_2021/mjmhs_vol17_supp_10_december_2021-64369 |
| spellingShingle | Othman, Rosfayati Mohtaruddin, Norhafizah Ahmad Zubir, Nurul Mahirah Seow, Heng Fong Ngan, Kah Wai Osman, Malina Upregulation and hypomethylation of EGFR in Formalin-fixed Paraffin Embedded FFPE tissues of colon adenocarcinoma |
| title | Upregulation and hypomethylation of EGFR in Formalin-fixed Paraffin Embedded FFPE tissues of colon adenocarcinoma |
| title_full | Upregulation and hypomethylation of EGFR in Formalin-fixed Paraffin Embedded FFPE tissues of colon adenocarcinoma |
| title_fullStr | Upregulation and hypomethylation of EGFR in Formalin-fixed Paraffin Embedded FFPE tissues of colon adenocarcinoma |
| title_full_unstemmed | Upregulation and hypomethylation of EGFR in Formalin-fixed Paraffin Embedded FFPE tissues of colon adenocarcinoma |
| title_short | Upregulation and hypomethylation of EGFR in Formalin-fixed Paraffin Embedded FFPE tissues of colon adenocarcinoma |
| title_sort | upregulation and hypomethylation of egfr in formalin fixed paraffin embedded ffpe tissues of colon adenocarcinoma |
| url | http://psasir.upm.edu.my/id/eprint/93494/1/2021120708131401_MJMHS_0571.pdf |
| work_keys_str_mv | AT othmanrosfayati upregulationandhypomethylationofegfrinformalinfixedparaffinembeddedffpetissuesofcolonadenocarcinoma AT mohtaruddinnorhafizah upregulationandhypomethylationofegfrinformalinfixedparaffinembeddedffpetissuesofcolonadenocarcinoma AT ahmadzubirnurulmahirah upregulationandhypomethylationofegfrinformalinfixedparaffinembeddedffpetissuesofcolonadenocarcinoma AT seowhengfong upregulationandhypomethylationofegfrinformalinfixedparaffinembeddedffpetissuesofcolonadenocarcinoma AT ngankahwai upregulationandhypomethylationofegfrinformalinfixedparaffinembeddedffpetissuesofcolonadenocarcinoma AT osmanmalina upregulationandhypomethylationofegfrinformalinfixedparaffinembeddedffpetissuesofcolonadenocarcinoma |