Metabolite variations and antioxidant activity of Muntingia calabura leaves in response to different drying methods and ethanol ratios elucidated by NMR-based metabolomics
Introduction Muntingia calabura from the Muntingiaceae family has been documented for several medicinal uses. The combinations of drying treatment and extracting solvents for a plant species need to be determined and optimised to ensure that the extracts contain adequate amounts of the bioactive me...
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| Format: | Article |
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John Wiley & Sons
2021
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| author | Zolkeflee, Nur Khaleeda Zulaikha Isamail, Nor Amira Maulidiani, Maulidiani Abdul Hamid, Nur Ashikin Ramli, Nurul Shazini Azlan, Azrina Abas, Faridah |
| author_facet | Zolkeflee, Nur Khaleeda Zulaikha Isamail, Nor Amira Maulidiani, Maulidiani Abdul Hamid, Nur Ashikin Ramli, Nurul Shazini Azlan, Azrina Abas, Faridah |
| author_sort | Zolkeflee, Nur Khaleeda Zulaikha |
| collection | UPM |
| description | Introduction
Muntingia calabura from the Muntingiaceae family has been documented for several medicinal uses. The combinations of drying treatment and extracting solvents for a plant species need to be determined and optimised to ensure that the extracts contain adequate amounts of the bioactive metabolites.
Objective
Evaluate the metabolite variations and antioxidant activity among M. calabura leaves subjected to different drying methods and extracted with different ethanol ratios using proton nuclear magnetic resonance (1H-NMR)-based metabolomics.
Methodology
The antioxidant activity of M. calabura leaves dried with three different drying methods and extracted with three different ethanol ratios was determined by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide (NO) scavenging assays. The metabolites variation among the extracts and correlation with antioxidant activity were analysed by 1H-NMR-based metabolomics.
Results
Muntingia calabura leaves extracted with 50% and 100% ethanol from air-drying and freeze-drying methods had the highest total phenolic content and the lowest IC50 value for the DPPH scavenging activity. Meanwhile, oven-dried leaves extracted with 100% ethanol had the lowest IC50 value for the NO scavenging activity. A total of 43 metabolites, including sugars, organic acids, amino acids, phytosterols, phenolics and terpene glycoside were tentatively identified. A noticeable discrimination was observed in the different ethanol ratios by the principal component analysis. The partial least-squares analysis suggested that 32 compounds out of 43 compounds identified were the contributors to the bioactivities.
Conclusion
The results established set the preliminary steps towards developing this plant into a high value product for phytomedicinal preparations. |
| first_indexed | 2024-03-06T10:59:05Z |
| format | Article |
| id | upm.eprints-94159 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T10:59:05Z |
| publishDate | 2021 |
| publisher | John Wiley & Sons |
| record_format | dspace |
| spelling | upm.eprints-941592023-03-28T06:18:40Z http://psasir.upm.edu.my/id/eprint/94159/ Metabolite variations and antioxidant activity of Muntingia calabura leaves in response to different drying methods and ethanol ratios elucidated by NMR-based metabolomics Zolkeflee, Nur Khaleeda Zulaikha Isamail, Nor Amira Maulidiani, Maulidiani Abdul Hamid, Nur Ashikin Ramli, Nurul Shazini Azlan, Azrina Abas, Faridah Introduction Muntingia calabura from the Muntingiaceae family has been documented for several medicinal uses. The combinations of drying treatment and extracting solvents for a plant species need to be determined and optimised to ensure that the extracts contain adequate amounts of the bioactive metabolites. Objective Evaluate the metabolite variations and antioxidant activity among M. calabura leaves subjected to different drying methods and extracted with different ethanol ratios using proton nuclear magnetic resonance (1H-NMR)-based metabolomics. Methodology The antioxidant activity of M. calabura leaves dried with three different drying methods and extracted with three different ethanol ratios was determined by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide (NO) scavenging assays. The metabolites variation among the extracts and correlation with antioxidant activity were analysed by 1H-NMR-based metabolomics. Results Muntingia calabura leaves extracted with 50% and 100% ethanol from air-drying and freeze-drying methods had the highest total phenolic content and the lowest IC50 value for the DPPH scavenging activity. Meanwhile, oven-dried leaves extracted with 100% ethanol had the lowest IC50 value for the NO scavenging activity. A total of 43 metabolites, including sugars, organic acids, amino acids, phytosterols, phenolics and terpene glycoside were tentatively identified. A noticeable discrimination was observed in the different ethanol ratios by the principal component analysis. The partial least-squares analysis suggested that 32 compounds out of 43 compounds identified were the contributors to the bioactivities. Conclusion The results established set the preliminary steps towards developing this plant into a high value product for phytomedicinal preparations. John Wiley & Sons 2021-01 Article PeerReviewed Zolkeflee, Nur Khaleeda Zulaikha and Isamail, Nor Amira and Maulidiani, Maulidiani and Abdul Hamid, Nur Ashikin and Ramli, Nurul Shazini and Azlan, Azrina and Abas, Faridah (2021) Metabolite variations and antioxidant activity of Muntingia calabura leaves in response to different drying methods and ethanol ratios elucidated by NMR-based metabolomics. Phytochemical Analysis, 32 (1). 69 - 83. ISSN 0958-0344; ESSN: 1099-1565 https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/pca.2917 10.1002/pca.2917 |
| spellingShingle | Zolkeflee, Nur Khaleeda Zulaikha Isamail, Nor Amira Maulidiani, Maulidiani Abdul Hamid, Nur Ashikin Ramli, Nurul Shazini Azlan, Azrina Abas, Faridah Metabolite variations and antioxidant activity of Muntingia calabura leaves in response to different drying methods and ethanol ratios elucidated by NMR-based metabolomics |
| title | Metabolite variations and antioxidant activity of Muntingia calabura leaves in response to different drying methods and ethanol ratios elucidated by NMR-based metabolomics |
| title_full | Metabolite variations and antioxidant activity of Muntingia calabura leaves in response to different drying methods and ethanol ratios elucidated by NMR-based metabolomics |
| title_fullStr | Metabolite variations and antioxidant activity of Muntingia calabura leaves in response to different drying methods and ethanol ratios elucidated by NMR-based metabolomics |
| title_full_unstemmed | Metabolite variations and antioxidant activity of Muntingia calabura leaves in response to different drying methods and ethanol ratios elucidated by NMR-based metabolomics |
| title_short | Metabolite variations and antioxidant activity of Muntingia calabura leaves in response to different drying methods and ethanol ratios elucidated by NMR-based metabolomics |
| title_sort | metabolite variations and antioxidant activity of muntingia calabura leaves in response to different drying methods and ethanol ratios elucidated by nmr based metabolomics |
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