Molecular characterisation and antibiotic sensitivity profile of Pasteurella multocida isolated from poultry farms in Malaysia
Fowl cholera has caused significant economic losses in many poultry producing countries worldwide. In Malaysia, outbreaks of fowl cholera are frequently reported and encountered in different types of poultry productions. The objective of this study was to characterise 13 avian Pasteurella multocida,...
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| Format: | Article |
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Facultad de Ciencias Veterinarias,Universidad Austral de Chile
2021
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| _version_ | 1796982947082928128 |
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| author | Sabsabi, Mohammad A. Sabsabi Zakaria, Zunita Abu, Jalila Nik M. Faiza |
| author_facet | Sabsabi, Mohammad A. Sabsabi Zakaria, Zunita Abu, Jalila Nik M. Faiza |
| author_sort | Sabsabi, Mohammad A. Sabsabi |
| collection | UPM |
| description | Fowl cholera has caused significant economic losses in many poultry producing countries worldwide. In Malaysia, outbreaks of fowl cholera are frequently reported and encountered in different types of poultry productions. The objective of this study was to characterise 13 avian Pasteurella multocida, isolated from fowl cholera outbreaks in Central Peninsular Malaysia in the period between 2000 and 2018. The isolates were subjected to multiplex polymerase chain reaction (PCR) for capsular serotyping, disc diffusion method for antimicrobial susceptibility profiles, and molecular genotyping using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The capsular serotyping showed all 13 Pasteurella multocida isolates belonging to capsular serotype A. The antimicrobial susceptibility showed several multidrug resistance strains among the P. multocida isolates. All the isolates were resistant to erythromycin (100%), streptomycin (68%), tetracycline (37%), enrofloxacin (37%), florfenicol (23%), penicillin G (14%), gentamicin (14%), and amoxicillin (14%). The PFGE analysis clustered the isolates into three clones. Group A included isolates with a similarity of 87% from the year 2000, 2013, and 2018. Three sequence types were identified using MLST typing namely, ST129, ST231, and ST355. The ST355 was assigned for the first time in the Rural Industries Research and Development Corporation (RIRDC) database. Besides, ST129 has been reported in India, China, and Sri Lanka, which highlights the possibility of transmission between Asian countries. This study provides an insight into epidemiological information of Pasteurella multocida that causes fowl cholera outbreaks in the central region of Peninsular Malaysia. |
| first_indexed | 2024-03-06T10:59:20Z |
| format | Article |
| id | upm.eprints-94243 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T10:59:20Z |
| publishDate | 2021 |
| publisher | Facultad de Ciencias Veterinarias,Universidad Austral de Chile |
| record_format | dspace |
| spelling | upm.eprints-942432023-05-09T01:56:15Z http://psasir.upm.edu.my/id/eprint/94243/ Molecular characterisation and antibiotic sensitivity profile of Pasteurella multocida isolated from poultry farms in Malaysia Sabsabi, Mohammad A. Sabsabi Zakaria, Zunita Abu, Jalila Nik M. Faiza Fowl cholera has caused significant economic losses in many poultry producing countries worldwide. In Malaysia, outbreaks of fowl cholera are frequently reported and encountered in different types of poultry productions. The objective of this study was to characterise 13 avian Pasteurella multocida, isolated from fowl cholera outbreaks in Central Peninsular Malaysia in the period between 2000 and 2018. The isolates were subjected to multiplex polymerase chain reaction (PCR) for capsular serotyping, disc diffusion method for antimicrobial susceptibility profiles, and molecular genotyping using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The capsular serotyping showed all 13 Pasteurella multocida isolates belonging to capsular serotype A. The antimicrobial susceptibility showed several multidrug resistance strains among the P. multocida isolates. All the isolates were resistant to erythromycin (100%), streptomycin (68%), tetracycline (37%), enrofloxacin (37%), florfenicol (23%), penicillin G (14%), gentamicin (14%), and amoxicillin (14%). The PFGE analysis clustered the isolates into three clones. Group A included isolates with a similarity of 87% from the year 2000, 2013, and 2018. Three sequence types were identified using MLST typing namely, ST129, ST231, and ST355. The ST355 was assigned for the first time in the Rural Industries Research and Development Corporation (RIRDC) database. Besides, ST129 has been reported in India, China, and Sri Lanka, which highlights the possibility of transmission between Asian countries. This study provides an insight into epidemiological information of Pasteurella multocida that causes fowl cholera outbreaks in the central region of Peninsular Malaysia. Facultad de Ciencias Veterinarias,Universidad Austral de Chile 2021 Article PeerReviewed Sabsabi, Mohammad A. Sabsabi and Zakaria, Zunita and Abu, Jalila and Nik M. Faiza (2021) Molecular characterisation and antibiotic sensitivity profile of Pasteurella multocida isolated from poultry farms in Malaysia. Austral journal of veterinary sciences, 53 (2). 121 - 126. ISSN 0719-8000; ESSN 0719-8132 https://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322021000200121&lng=en&nrm=iso&tlng=en 10.4067/S0719-81322021000200121 |
| spellingShingle | Sabsabi, Mohammad A. Sabsabi Zakaria, Zunita Abu, Jalila Nik M. Faiza Molecular characterisation and antibiotic sensitivity profile of Pasteurella multocida isolated from poultry farms in Malaysia |
| title | Molecular characterisation and antibiotic sensitivity profile of Pasteurella multocida isolated from poultry farms in Malaysia |
| title_full | Molecular characterisation and antibiotic sensitivity profile of Pasteurella multocida isolated from poultry farms in Malaysia |
| title_fullStr | Molecular characterisation and antibiotic sensitivity profile of Pasteurella multocida isolated from poultry farms in Malaysia |
| title_full_unstemmed | Molecular characterisation and antibiotic sensitivity profile of Pasteurella multocida isolated from poultry farms in Malaysia |
| title_short | Molecular characterisation and antibiotic sensitivity profile of Pasteurella multocida isolated from poultry farms in Malaysia |
| title_sort | molecular characterisation and antibiotic sensitivity profile of pasteurella multocida isolated from poultry farms in malaysia |
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