Molecular detection and antibiogram of Staphylococcus aureus in rabbits, rabbit handlers, and Rabbitry in Terengganu, Malaysia
Objectives: This study aims to investigate the prevalence and antibiogram of Staphylococcus aureus and methicillin-resistance S. aureus (MRSA) in rabbits, rabbit handlers, and rabbitry environments in Terengganu. Materials and Methods: Swab samples from 183 rabbits (183 oral and 183 ear swabs), 45...
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Network for the Veterinarians of Bangladesh
2021
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| author | Chai, Min Hian Sukiman, Muhammad Zikree Mohammad Najib, Nurlailasari Mohabbar, Nor Arifah Mohd Azizan, Nur Aina Nadhirah Mohamad, Noor Muzamil Zainal Ariffin, Siti Mariam Ghazali, Mohd Faizal |
| author_facet | Chai, Min Hian Sukiman, Muhammad Zikree Mohammad Najib, Nurlailasari Mohabbar, Nor Arifah Mohd Azizan, Nur Aina Nadhirah Mohamad, Noor Muzamil Zainal Ariffin, Siti Mariam Ghazali, Mohd Faizal |
| author_sort | Chai, Min Hian |
| collection | UPM |
| description | Objectives: This study aims to investigate the prevalence and antibiogram of Staphylococcus aureus and methicillin-resistance S. aureus (MRSA) in rabbits, rabbit handlers, and rabbitry environments in Terengganu.
Materials and Methods: Swab samples from 183 rabbits (183 oral and 183 ear swabs), 45 rabbit handlers (45 oral and 45 nasal), and environmental (n = 180) samples from rabbitries were collected from 10 rabbit farms in Terengganu. The associated S. aureus isolates from the swabs were isolated using phenotypic microbiology tests. The bacteria were confirmed by polymerase chain reaction targeting nuc (S. aureus) and mecA (MRSA) genes. The antibiogram of all S. aureus isolates was determined using the Kirby–Bauer test.
Results: Staphylococcus aureus was detected in 19% of rabbits, 26.7% of rabbit handlers, and 8.8% of swabs from the rabbitry environment. However, MRSA (0%) could not be detected. Antibiotic susceptibility test revealed that S. aureus from rabbits showed low resistance (<20%) against 15 different antibiotics while fully susceptible to 4 antibiotics. Meanwhile, S. aureus from rabbit handlers showed high resistance against penicillin (86%), oxacillin (64%), and amoxicillin (50%).
Conclusions: This study suggests the emergence of antibiotic-resistant S. aureus in rabbit farms settings. Therefore, careful selection of antimicrobial agents will be essential to preserve the effectiveness of treatments toward S. aureus infections. |
| first_indexed | 2024-03-06T10:59:20Z |
| format | Article |
| id | upm.eprints-94247 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T10:59:20Z |
| publishDate | 2021 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | dspace |
| spelling | upm.eprints-942472023-05-09T02:00:48Z http://psasir.upm.edu.my/id/eprint/94247/ Molecular detection and antibiogram of Staphylococcus aureus in rabbits, rabbit handlers, and Rabbitry in Terengganu, Malaysia Chai, Min Hian Sukiman, Muhammad Zikree Mohammad Najib, Nurlailasari Mohabbar, Nor Arifah Mohd Azizan, Nur Aina Nadhirah Mohamad, Noor Muzamil Zainal Ariffin, Siti Mariam Ghazali, Mohd Faizal Objectives: This study aims to investigate the prevalence and antibiogram of Staphylococcus aureus and methicillin-resistance S. aureus (MRSA) in rabbits, rabbit handlers, and rabbitry environments in Terengganu. Materials and Methods: Swab samples from 183 rabbits (183 oral and 183 ear swabs), 45 rabbit handlers (45 oral and 45 nasal), and environmental (n = 180) samples from rabbitries were collected from 10 rabbit farms in Terengganu. The associated S. aureus isolates from the swabs were isolated using phenotypic microbiology tests. The bacteria were confirmed by polymerase chain reaction targeting nuc (S. aureus) and mecA (MRSA) genes. The antibiogram of all S. aureus isolates was determined using the Kirby–Bauer test. Results: Staphylococcus aureus was detected in 19% of rabbits, 26.7% of rabbit handlers, and 8.8% of swabs from the rabbitry environment. However, MRSA (0%) could not be detected. Antibiotic susceptibility test revealed that S. aureus from rabbits showed low resistance (<20%) against 15 different antibiotics while fully susceptible to 4 antibiotics. Meanwhile, S. aureus from rabbit handlers showed high resistance against penicillin (86%), oxacillin (64%), and amoxicillin (50%). Conclusions: This study suggests the emergence of antibiotic-resistant S. aureus in rabbit farms settings. Therefore, careful selection of antimicrobial agents will be essential to preserve the effectiveness of treatments toward S. aureus infections. Network for the Veterinarians of Bangladesh 2021-09-19 Article PeerReviewed Chai, Min Hian and Sukiman, Muhammad Zikree and Mohammad Najib, Nurlailasari and Mohabbar, Nor Arifah and Mohd Azizan, Nur Aina Nadhirah and Mohamad, Noor Muzamil and Zainal Ariffin, Siti Mariam and Ghazali, Mohd Faizal (2021) Molecular detection and antibiogram of Staphylococcus aureus in rabbits, rabbit handlers, and Rabbitry in Terengganu, Malaysia. Journal of Advanced Veterinary and Animal Research, 8 (3). 388 - 395. ISSN 2311-7710 https://bdvets.org/JAVAR/issue-v8i321.html 10.5455/javar.2021.h527 |
| spellingShingle | Chai, Min Hian Sukiman, Muhammad Zikree Mohammad Najib, Nurlailasari Mohabbar, Nor Arifah Mohd Azizan, Nur Aina Nadhirah Mohamad, Noor Muzamil Zainal Ariffin, Siti Mariam Ghazali, Mohd Faizal Molecular detection and antibiogram of Staphylococcus aureus in rabbits, rabbit handlers, and Rabbitry in Terengganu, Malaysia |
| title | Molecular detection and antibiogram of Staphylococcus aureus in rabbits, rabbit handlers, and Rabbitry in Terengganu, Malaysia |
| title_full | Molecular detection and antibiogram of Staphylococcus aureus in rabbits, rabbit handlers, and Rabbitry in Terengganu, Malaysia |
| title_fullStr | Molecular detection and antibiogram of Staphylococcus aureus in rabbits, rabbit handlers, and Rabbitry in Terengganu, Malaysia |
| title_full_unstemmed | Molecular detection and antibiogram of Staphylococcus aureus in rabbits, rabbit handlers, and Rabbitry in Terengganu, Malaysia |
| title_short | Molecular detection and antibiogram of Staphylococcus aureus in rabbits, rabbit handlers, and Rabbitry in Terengganu, Malaysia |
| title_sort | molecular detection and antibiogram of staphylococcus aureus in rabbits rabbit handlers and rabbitry in terengganu malaysia |
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