Muscle and serum metabolomics for different chicken breeds under commercial conditions by GC–MS

Globally, village chicken is popular and is known as a premium meat with a higher price. Food fraud can occur by selling other chicken breeds at a premium price in local markets. This study aimed to distinguish local village chicken from other chicken breeds available in the market, namely, colored...

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Main Authors: Tan, Cheng keng, Selamat, Jinap, Jambari, Nuzul Noorahya, Sukor, Rashidah, Murugesu, Suganya, Khatib, Alfi
Format: Article
Published: MDPI 2021
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author Tan, Cheng keng
Selamat, Jinap
Jambari, Nuzul Noorahya
Sukor, Rashidah
Murugesu, Suganya
Khatib, Alfi
author_facet Tan, Cheng keng
Selamat, Jinap
Jambari, Nuzul Noorahya
Sukor, Rashidah
Murugesu, Suganya
Khatib, Alfi
author_sort Tan, Cheng keng
collection UPM
description Globally, village chicken is popular and is known as a premium meat with a higher price. Food fraud can occur by selling other chicken breeds at a premium price in local markets. This study aimed to distinguish local village chicken from other chicken breeds available in the market, namely, colored broiler (Hubbard), broiler (Cobb), and spent laying hen (Dekalb) in pectoralis major and serum under commercial conditions using an untargeted metabolomics approach. Both pectoralis major and serum were analyzed using gas chromatography–mass spectrometry (GC–MS). The principal component analysis (PCA) results distinguished four different chicken breeds into three main groups for pectoralis major and serum. A total of 30 and 40 characteristic metabolites were identified for pectoralis major and serum, respectively. The four chicken breeds were characterized by the abundance of metabolites such as amino acids (L−glutamic acid, L−threonine, L−serine, L−leucine), organic acids (L−lactic acid, succinic acid, 3−hydroxybutyric acid), sugars (D−allose, D−glucose), sugar alcohols (myo−inositol), and fatty acids (linoleic acid). Our results suggest that an untargeted metabolomics approach using GC–MS and PCA could discriminate chicken breeds for pectoralis major and serum under commercial conditions. In this study, village chicken could only be distinguished from colored broiler (Hubbard) by serum samples.
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spelling upm.eprints-943062023-05-08T03:08:05Z http://psasir.upm.edu.my/id/eprint/94306/ Muscle and serum metabolomics for different chicken breeds under commercial conditions by GC–MS Tan, Cheng keng Selamat, Jinap Jambari, Nuzul Noorahya Sukor, Rashidah Murugesu, Suganya Khatib, Alfi Globally, village chicken is popular and is known as a premium meat with a higher price. Food fraud can occur by selling other chicken breeds at a premium price in local markets. This study aimed to distinguish local village chicken from other chicken breeds available in the market, namely, colored broiler (Hubbard), broiler (Cobb), and spent laying hen (Dekalb) in pectoralis major and serum under commercial conditions using an untargeted metabolomics approach. Both pectoralis major and serum were analyzed using gas chromatography–mass spectrometry (GC–MS). The principal component analysis (PCA) results distinguished four different chicken breeds into three main groups for pectoralis major and serum. A total of 30 and 40 characteristic metabolites were identified for pectoralis major and serum, respectively. The four chicken breeds were characterized by the abundance of metabolites such as amino acids (L−glutamic acid, L−threonine, L−serine, L−leucine), organic acids (L−lactic acid, succinic acid, 3−hydroxybutyric acid), sugars (D−allose, D−glucose), sugar alcohols (myo−inositol), and fatty acids (linoleic acid). Our results suggest that an untargeted metabolomics approach using GC–MS and PCA could discriminate chicken breeds for pectoralis major and serum under commercial conditions. In this study, village chicken could only be distinguished from colored broiler (Hubbard) by serum samples. MDPI 2021-09-14 Article PeerReviewed Tan, Cheng keng and Selamat, Jinap and Jambari, Nuzul Noorahya and Sukor, Rashidah and Murugesu, Suganya and Khatib, Alfi (2021) Muscle and serum metabolomics for different chicken breeds under commercial conditions by GC–MS. Foods, 10 (9). art. no. 2174. pp. 1-17. ISSN 2304-8158 https://www.mdpi.com/2304-8158/10/9/2174 10.3390/foods10092174
spellingShingle Tan, Cheng keng
Selamat, Jinap
Jambari, Nuzul Noorahya
Sukor, Rashidah
Murugesu, Suganya
Khatib, Alfi
Muscle and serum metabolomics for different chicken breeds under commercial conditions by GC–MS
title Muscle and serum metabolomics for different chicken breeds under commercial conditions by GC–MS
title_full Muscle and serum metabolomics for different chicken breeds under commercial conditions by GC–MS
title_fullStr Muscle and serum metabolomics for different chicken breeds under commercial conditions by GC–MS
title_full_unstemmed Muscle and serum metabolomics for different chicken breeds under commercial conditions by GC–MS
title_short Muscle and serum metabolomics for different chicken breeds under commercial conditions by GC–MS
title_sort muscle and serum metabolomics for different chicken breeds under commercial conditions by gc ms
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AT selamatjinap muscleandserummetabolomicsfordifferentchickenbreedsundercommercialconditionsbygcms
AT jambarinuzulnoorahya muscleandserummetabolomicsfordifferentchickenbreedsundercommercialconditionsbygcms
AT sukorrashidah muscleandserummetabolomicsfordifferentchickenbreedsundercommercialconditionsbygcms
AT murugesusuganya muscleandserummetabolomicsfordifferentchickenbreedsundercommercialconditionsbygcms
AT khatibalfi muscleandserummetabolomicsfordifferentchickenbreedsundercommercialconditionsbygcms