Octreotide acetate enhanced radio sensitivity and induced apoptosis in MCF7 breast cancer cell line
Introduction: Breast cancer is the most common type of cancer among women. Radiotherapy is one of the treatment options for breast cancer; it plays a significant role in tumour control after surgery. However, radio resistance developed by tumour cells is the main problem in radiotherapy. As such, ra...
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| Format: | Article |
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Elsevier
2021
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| _version_ | 1825937458941919232 |
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| author | Salih Mahmoud, Alkhansa Abu Bakar @ Zakaria, Md Zuki Hamzah, Hazilawati Tengku Ahmad, Tengku Ahbrizal Farizal Mohd Noor, Mohd Hezmee |
| author_facet | Salih Mahmoud, Alkhansa Abu Bakar @ Zakaria, Md Zuki Hamzah, Hazilawati Tengku Ahmad, Tengku Ahbrizal Farizal Mohd Noor, Mohd Hezmee |
| author_sort | Salih Mahmoud, Alkhansa |
| collection | UPM |
| description | Introduction: Breast cancer is the most common type of cancer among women. Radiotherapy is one of the treatment options for breast cancer; it plays a significant role in tumour control after surgery. However, radio resistance developed by tumour cells is the main problem in radiotherapy. As such, radiosensitization agents are used to improve radiotherapy efficiency. This study aims to investigate the role of octreotide acetate as a radiosensitizer for MCF7 cells.
Methods: The treatments were designated into four groups: untreated cells, cells treated with octreotide acetate,
cells treated with X-ray and cells treated with octreotide acetate in combination with X-ray irradiation. Cell viability assay, clonogenic assay, and qPCR was used as BAX apoptosis indicator marker analysis Morphological changes were examined under an inverted microscope.
Results: The results showed more effectiveness for the octreotide acetate combination to X-ray radiation in the
MCF7 cell line.
Conclusion: These findings suggested that octreotide acetate enhanced radiosensitivity and induced apoptosis in
MCF7 cell line. |
| first_indexed | 2024-03-06T10:59:49Z |
| format | Article |
| id | upm.eprints-94414 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T10:59:49Z |
| publishDate | 2021 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-944142023-03-29T07:43:54Z http://psasir.upm.edu.my/id/eprint/94414/ Octreotide acetate enhanced radio sensitivity and induced apoptosis in MCF7 breast cancer cell line Salih Mahmoud, Alkhansa Abu Bakar @ Zakaria, Md Zuki Hamzah, Hazilawati Tengku Ahmad, Tengku Ahbrizal Farizal Mohd Noor, Mohd Hezmee Introduction: Breast cancer is the most common type of cancer among women. Radiotherapy is one of the treatment options for breast cancer; it plays a significant role in tumour control after surgery. However, radio resistance developed by tumour cells is the main problem in radiotherapy. As such, radiosensitization agents are used to improve radiotherapy efficiency. This study aims to investigate the role of octreotide acetate as a radiosensitizer for MCF7 cells. Methods: The treatments were designated into four groups: untreated cells, cells treated with octreotide acetate, cells treated with X-ray and cells treated with octreotide acetate in combination with X-ray irradiation. Cell viability assay, clonogenic assay, and qPCR was used as BAX apoptosis indicator marker analysis Morphological changes were examined under an inverted microscope. Results: The results showed more effectiveness for the octreotide acetate combination to X-ray radiation in the MCF7 cell line. Conclusion: These findings suggested that octreotide acetate enhanced radiosensitivity and induced apoptosis in MCF7 cell line. Elsevier 2021-03 Article PeerReviewed Salih Mahmoud, Alkhansa and Abu Bakar @ Zakaria, Md Zuki and Hamzah, Hazilawati and Tengku Ahmad, Tengku Ahbrizal Farizal and Mohd Noor, Mohd Hezmee (2021) Octreotide acetate enhanced radio sensitivity and induced apoptosis in MCF7 breast cancer cell line. Journal of Radiation Research and Applied Sciences, 15 (1). pp. 193-198. ISSN 1687-8507 https://www.sciencedirect.com/science/article/pii/S168785072200108X 10.1016/j.jrras.2022.01.008 |
| spellingShingle | Salih Mahmoud, Alkhansa Abu Bakar @ Zakaria, Md Zuki Hamzah, Hazilawati Tengku Ahmad, Tengku Ahbrizal Farizal Mohd Noor, Mohd Hezmee Octreotide acetate enhanced radio sensitivity and induced apoptosis in MCF7 breast cancer cell line |
| title | Octreotide acetate enhanced radio sensitivity and induced apoptosis in MCF7 breast cancer cell line |
| title_full | Octreotide acetate enhanced radio sensitivity and induced apoptosis in MCF7 breast cancer cell line |
| title_fullStr | Octreotide acetate enhanced radio sensitivity and induced apoptosis in MCF7 breast cancer cell line |
| title_full_unstemmed | Octreotide acetate enhanced radio sensitivity and induced apoptosis in MCF7 breast cancer cell line |
| title_short | Octreotide acetate enhanced radio sensitivity and induced apoptosis in MCF7 breast cancer cell line |
| title_sort | octreotide acetate enhanced radio sensitivity and induced apoptosis in mcf7 breast cancer cell line |
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