Pathogenicity and immunogenicity of attenuated fowl adenovirus from chicken embryo liver cells in commercial broiler chickens
Fowl adenovirus (FAdV) infection is a major viral threat to poultry industry worldwide. The virus cause Inclusion body hepatitis (IBH) outbreaks with serious economic losses and it demands an effective prevention and control measures of the disease. The objectives of this study were to determine pat...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Published: |
Nexus Academic Publishers
2021
|
| _version_ | 1796982995852197888 |
|---|---|
| author | Mohamed Sohaimi, Norfitriah Bejo, Mohd Hair Omar, Abdul Rahman Ideris, Aini Mat Isa, Nurulfiza |
| author_facet | Mohamed Sohaimi, Norfitriah Bejo, Mohd Hair Omar, Abdul Rahman Ideris, Aini Mat Isa, Nurulfiza |
| author_sort | Mohamed Sohaimi, Norfitriah |
| collection | UPM |
| description | Fowl adenovirus (FAdV) infection is a major viral threat to poultry industry worldwide. The virus cause Inclusion body hepatitis (IBH) outbreaks with serious economic losses and it demands an effective prevention and control measures of the disease. The objectives of this study were to determine pathogenicity and immunogenicity of live attenuated FAdV isolate (UPM1137) in commercial broiler chickens. The FAdV isolate was propagated into primary chicken embryo liver (CEL) cells for 35th passages (UPM1137CEL35) and induced several molecular changes in hexon and fiber genes at capsid region of viral protein. Sixty-four 1 day-old commercial broiler chickens were used and divided into three groups, 20 chicks in the groups A and B, and 24 chicks in the group C. The isolate with titer of 106.7TCID/ml was inoculated (0.5mL) into one-day-old chicks either via oral (Group A) or intraperitoneal (Group B) route. Control (Group C) was included and remained uninoculated. Samples of serum, liver and gizzard were collected in the chickens at day 0 post inoculation (pi) in the Control group and at days 3, 7, 14 and 21 pi in all groups. The study demonstrated neither clinical signs, nor gross and histological lesions were observed in all chickens. The isolate induced a significantly (p<0.05) high FAdV antibody titre (2348 ± 1800) at day 21pi in Group B (intraperitoneal) when compared to the Group A (oral) (190 ± 136) in the present of maternal derived antibody (MDA) (7795 ± 1414 at day-old). High antibody response at day 21pi via intraperitoneal route indicates that the viral antigen capable to circumvent MDA from neutralization due to changes in epitopes in L1 loop of hexon and knob of fiber genes. Thus, the live attenuated FAdV isolate (UPM1137CEL35) has high potential for future use against FAdV serotype 8b infection in the chickens. |
| first_indexed | 2024-03-06T11:00:03Z |
| format | Article |
| id | upm.eprints-94497 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T11:00:03Z |
| publishDate | 2021 |
| publisher | Nexus Academic Publishers |
| record_format | dspace |
| spelling | upm.eprints-944972022-11-29T07:29:03Z http://psasir.upm.edu.my/id/eprint/94497/ Pathogenicity and immunogenicity of attenuated fowl adenovirus from chicken embryo liver cells in commercial broiler chickens Mohamed Sohaimi, Norfitriah Bejo, Mohd Hair Omar, Abdul Rahman Ideris, Aini Mat Isa, Nurulfiza Fowl adenovirus (FAdV) infection is a major viral threat to poultry industry worldwide. The virus cause Inclusion body hepatitis (IBH) outbreaks with serious economic losses and it demands an effective prevention and control measures of the disease. The objectives of this study were to determine pathogenicity and immunogenicity of live attenuated FAdV isolate (UPM1137) in commercial broiler chickens. The FAdV isolate was propagated into primary chicken embryo liver (CEL) cells for 35th passages (UPM1137CEL35) and induced several molecular changes in hexon and fiber genes at capsid region of viral protein. Sixty-four 1 day-old commercial broiler chickens were used and divided into three groups, 20 chicks in the groups A and B, and 24 chicks in the group C. The isolate with titer of 106.7TCID/ml was inoculated (0.5mL) into one-day-old chicks either via oral (Group A) or intraperitoneal (Group B) route. Control (Group C) was included and remained uninoculated. Samples of serum, liver and gizzard were collected in the chickens at day 0 post inoculation (pi) in the Control group and at days 3, 7, 14 and 21 pi in all groups. The study demonstrated neither clinical signs, nor gross and histological lesions were observed in all chickens. The isolate induced a significantly (p<0.05) high FAdV antibody titre (2348 ± 1800) at day 21pi in Group B (intraperitoneal) when compared to the Group A (oral) (190 ± 136) in the present of maternal derived antibody (MDA) (7795 ± 1414 at day-old). High antibody response at day 21pi via intraperitoneal route indicates that the viral antigen capable to circumvent MDA from neutralization due to changes in epitopes in L1 loop of hexon and knob of fiber genes. Thus, the live attenuated FAdV isolate (UPM1137CEL35) has high potential for future use against FAdV serotype 8b infection in the chickens. Nexus Academic Publishers 2021-03 Article PeerReviewed Mohamed Sohaimi, Norfitriah and Bejo, Mohd Hair and Omar, Abdul Rahman and Ideris, Aini and Mat Isa, Nurulfiza (2021) Pathogenicity and immunogenicity of attenuated fowl adenovirus from chicken embryo liver cells in commercial broiler chickens. Advances in Animal and Veterinary Sciences, 9 (5). 648 - 654. ISSN 2309-3331; ESSN: 2307-8316 https://nexusacademicpublishers.com/table_contents_detail/4/1747 10.17582/journal.aavs/2021/9.5.648.654 |
| spellingShingle | Mohamed Sohaimi, Norfitriah Bejo, Mohd Hair Omar, Abdul Rahman Ideris, Aini Mat Isa, Nurulfiza Pathogenicity and immunogenicity of attenuated fowl adenovirus from chicken embryo liver cells in commercial broiler chickens |
| title | Pathogenicity and immunogenicity of attenuated fowl adenovirus from chicken embryo liver cells in commercial broiler chickens |
| title_full | Pathogenicity and immunogenicity of attenuated fowl adenovirus from chicken embryo liver cells in commercial broiler chickens |
| title_fullStr | Pathogenicity and immunogenicity of attenuated fowl adenovirus from chicken embryo liver cells in commercial broiler chickens |
| title_full_unstemmed | Pathogenicity and immunogenicity of attenuated fowl adenovirus from chicken embryo liver cells in commercial broiler chickens |
| title_short | Pathogenicity and immunogenicity of attenuated fowl adenovirus from chicken embryo liver cells in commercial broiler chickens |
| title_sort | pathogenicity and immunogenicity of attenuated fowl adenovirus from chicken embryo liver cells in commercial broiler chickens |
| work_keys_str_mv | AT mohamedsohaiminorfitriah pathogenicityandimmunogenicityofattenuatedfowladenovirusfromchickenembryolivercellsincommercialbroilerchickens AT bejomohdhair pathogenicityandimmunogenicityofattenuatedfowladenovirusfromchickenembryolivercellsincommercialbroilerchickens AT omarabdulrahman pathogenicityandimmunogenicityofattenuatedfowladenovirusfromchickenembryolivercellsincommercialbroilerchickens AT iderisaini pathogenicityandimmunogenicityofattenuatedfowladenovirusfromchickenembryolivercellsincommercialbroilerchickens AT matisanurulfiza pathogenicityandimmunogenicityofattenuatedfowladenovirusfromchickenembryolivercellsincommercialbroilerchickens |