Statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from Antarctica
The extensive industrial use of the heavy metal molybdenum (Mo) has led to an emerging global pollution with its traces that can even be found in Antarctica. In response, a reduction process that transforms hexamolybdate (Mo6+) to a less toxic compound, Mo-blue, using microorganisms provides a susta...
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| Format: | Article |
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MDPI AG
2021
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| _version_ | 1825937523911688192 |
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| author | Darham, Syazani Mohd Zahri, Khadijah Nabilah Zulkharnain, Azham Sabri, Suriana Gomez-Fuentes, Claudio Convey, Peter Abdul Khalil, Khalilah Ahmad, Siti Aqlima |
| author_facet | Darham, Syazani Mohd Zahri, Khadijah Nabilah Zulkharnain, Azham Sabri, Suriana Gomez-Fuentes, Claudio Convey, Peter Abdul Khalil, Khalilah Ahmad, Siti Aqlima |
| author_sort | Darham, Syazani |
| collection | UPM |
| description | The extensive industrial use of the heavy metal molybdenum (Mo) has led to an emerging global pollution with its traces that can even be found in Antarctica. In response, a reduction process that transforms hexamolybdate (Mo6+) to a less toxic compound, Mo-blue, using microorganisms provides a sustainable remediation approach. The aim of this study was to investigate the reduction of Mo by a psychrotolerant Antarctic marine bacterium, Marinomonas sp. strain AQ5-A9. Mo reduction was optimised using One-Factor-At-a-Time (OFAT) and Response Surface Methodology (RSM). Subsequently, Mo reduction kinetics were further studied. OFAT results showed that maximum Mo reduction occurred in culture media conditions of pH 6.0 and 50 ppt salinity at 15 °C, with initial sucrose, nitrogen and molybdate concentrations of 2.0%, 3.0 g/L and 10 mM, respectively. Further optimization using RSM identified improved optimum conditions of pH 6.0 and 47 ppt salinity at 16 °C, with initial sucrose, nitrogen and molybdate concentrations of 1.8%, 2.25 g/L and 16 mM, respectively. Investigation of the kinetics of Mo reduction revealed Aiba as the best-fitting model. The calculated Aiba coefficient of maximum Mo reduction rate (µmax) was 0.067 h−1. The data obtained support the potential use of marine bacteria in the bioremediation of Mo. |
| first_indexed | 2024-03-06T11:00:48Z |
| format | Article |
| id | upm.eprints-95153 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T11:00:48Z |
| publishDate | 2021 |
| publisher | MDPI AG |
| record_format | dspace |
| spelling | upm.eprints-951532023-01-09T03:47:41Z http://psasir.upm.edu.my/id/eprint/95153/ Statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from Antarctica Darham, Syazani Mohd Zahri, Khadijah Nabilah Zulkharnain, Azham Sabri, Suriana Gomez-Fuentes, Claudio Convey, Peter Abdul Khalil, Khalilah Ahmad, Siti Aqlima The extensive industrial use of the heavy metal molybdenum (Mo) has led to an emerging global pollution with its traces that can even be found in Antarctica. In response, a reduction process that transforms hexamolybdate (Mo6+) to a less toxic compound, Mo-blue, using microorganisms provides a sustainable remediation approach. The aim of this study was to investigate the reduction of Mo by a psychrotolerant Antarctic marine bacterium, Marinomonas sp. strain AQ5-A9. Mo reduction was optimised using One-Factor-At-a-Time (OFAT) and Response Surface Methodology (RSM). Subsequently, Mo reduction kinetics were further studied. OFAT results showed that maximum Mo reduction occurred in culture media conditions of pH 6.0 and 50 ppt salinity at 15 °C, with initial sucrose, nitrogen and molybdate concentrations of 2.0%, 3.0 g/L and 10 mM, respectively. Further optimization using RSM identified improved optimum conditions of pH 6.0 and 47 ppt salinity at 16 °C, with initial sucrose, nitrogen and molybdate concentrations of 1.8%, 2.25 g/L and 16 mM, respectively. Investigation of the kinetics of Mo reduction revealed Aiba as the best-fitting model. The calculated Aiba coefficient of maximum Mo reduction rate (µmax) was 0.067 h−1. The data obtained support the potential use of marine bacteria in the bioremediation of Mo. MDPI AG 2021-06-11 Article PeerReviewed Darham, Syazani and Mohd Zahri, Khadijah Nabilah and Zulkharnain, Azham and Sabri, Suriana and Gomez-Fuentes, Claudio and Convey, Peter and Abdul Khalil, Khalilah and Ahmad, Siti Aqlima (2021) Statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from Antarctica. Journal of Marine Science and Engineering, 9 (6). art. no. 648. pp. 1-17. ISSN 2077-1312 https://www.mdpi.com/2077-1312/9/6/648/htm 10.3390/jmse9060648 |
| spellingShingle | Darham, Syazani Mohd Zahri, Khadijah Nabilah Zulkharnain, Azham Sabri, Suriana Gomez-Fuentes, Claudio Convey, Peter Abdul Khalil, Khalilah Ahmad, Siti Aqlima Statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from Antarctica |
| title | Statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from Antarctica |
| title_full | Statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from Antarctica |
| title_fullStr | Statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from Antarctica |
| title_full_unstemmed | Statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from Antarctica |
| title_short | Statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from Antarctica |
| title_sort | statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from antarctica |
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