Isolation, Characterization and Application of Micro Satellite Markers in the Southeast Asian River Catfish (Baung) Mystus Nemurus (C&V)
In Malaysia 70% of the dietary protein comes from fish. The popularity of the river catfish Mystus nemurus throughout Malaysia and also Southeast Asia has made it an important aquaculture candidate. Among the vast choice of molecular markers available to carry out a population study including sto...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2002
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| Online Access: | http://psasir.upm.edu.my/id/eprint/9519/1/FSAS_2002_48_A.pdf |
| Summary: | In Malaysia 70% of the dietary protein comes from fish. The popularity of the river
catfish Mystus nemurus throughout Malaysia and also Southeast Asia has made it an
important aquaculture candidate. Among the vast choice of molecular markers
available to carry out a population study including stock identification and
improvement, microsatellites have proven to be the most efficient.
The main objectives of this study were: (1) to develop efficient microsatellite
isolation protocols for Mystus nemurus followed by a population study using the newly developed microsatellite markers and (2) to test two new techniques, direct
amplification of length polymorphism (DALP) and random hybridising
microsatellites (RAHM), as potential DNA fingerprinting markers for population
studies. |
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