Performance of single and two-stage cross-flow ultrafiltration membrane in fractionation of peptide from microalgae protein hydrolysate (nannochloropsis gaditana)
Cross-flow ultrafiltration (UF) membrane with two different configurations; single (10 kDa and 5 kDa) and two-stage (10/5 kDa) in fractionating microalgae protein hydrolysate (MPH) were studied to obtain a low molecular weight of peptide. The effect of flow rate, trans-membrane pressure (TMP), and p...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/95556/1/Performance%20of%20single%20and%20two-stage%20cross-flow%20ultrafiltration%20membrane%20in%20fractionation%20of%20peptide%20from%20microalgae%20protein%20hydrolysate%20%28nannochloropsis%20gaditana%29.pdf |
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| author | Md Saleh, Nur Izzati Wan Ab Karim Ghani, Wan Azlina Mustapa Kamal, Siti Mazlina Harun, Razif |
| author_facet | Md Saleh, Nur Izzati Wan Ab Karim Ghani, Wan Azlina Mustapa Kamal, Siti Mazlina Harun, Razif |
| author_sort | Md Saleh, Nur Izzati |
| collection | UPM |
| description | Cross-flow ultrafiltration (UF) membrane with two different configurations; single (10 kDa and 5 kDa) and two-stage (10/5 kDa) in fractionating microalgae protein hydrolysate (MPH) were studied to obtain a low molecular weight of peptide. The effect of flow rate, trans-membrane pressure (TMP), and pH in fractionating MPH were evaluated based on permeate flux and peptide transmission. The results showed that, for single UF membrane, optimum operating parameters were at a flow rate of 23 mL/min, TMP of 1.5 bar, and pH of 9, with permeate flux of 43.65 L/m2 h (10 kDa) and 55.42 L/m2 h (5 kDa) and peptide transmission of 58.20% (10 kDa) and 67.34% (5 kDa). Meanwhile, for two-stage (10/5 kDa) UF membrane, the best parameters were observed at a flow rate of 23 mL/min, TMP of 1.5 bar, and pH of 2, with permeate flux of 69.85 L/m2 h and peptide transmission of 79.13%. Fractionation of MPH with two-stage UF membrane was observed to be better at producing a low molecular weight of peptide compared to single UF membrane. In conclusion, it was possible to produce permeate flux with a high amount of low molecular weight of peptide by controlling the operating parameters with the suitable configuration membrane. |
| first_indexed | 2024-03-06T11:01:54Z |
| format | Article |
| id | upm.eprints-95556 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T11:01:54Z |
| publishDate | 2021 |
| publisher | MDPI AG |
| record_format | dspace |
| spelling | upm.eprints-955562022-08-02T07:09:33Z http://psasir.upm.edu.my/id/eprint/95556/ Performance of single and two-stage cross-flow ultrafiltration membrane in fractionation of peptide from microalgae protein hydrolysate (nannochloropsis gaditana) Md Saleh, Nur Izzati Wan Ab Karim Ghani, Wan Azlina Mustapa Kamal, Siti Mazlina Harun, Razif Cross-flow ultrafiltration (UF) membrane with two different configurations; single (10 kDa and 5 kDa) and two-stage (10/5 kDa) in fractionating microalgae protein hydrolysate (MPH) were studied to obtain a low molecular weight of peptide. The effect of flow rate, trans-membrane pressure (TMP), and pH in fractionating MPH were evaluated based on permeate flux and peptide transmission. The results showed that, for single UF membrane, optimum operating parameters were at a flow rate of 23 mL/min, TMP of 1.5 bar, and pH of 9, with permeate flux of 43.65 L/m2 h (10 kDa) and 55.42 L/m2 h (5 kDa) and peptide transmission of 58.20% (10 kDa) and 67.34% (5 kDa). Meanwhile, for two-stage (10/5 kDa) UF membrane, the best parameters were observed at a flow rate of 23 mL/min, TMP of 1.5 bar, and pH of 2, with permeate flux of 69.85 L/m2 h and peptide transmission of 79.13%. Fractionation of MPH with two-stage UF membrane was observed to be better at producing a low molecular weight of peptide compared to single UF membrane. In conclusion, it was possible to produce permeate flux with a high amount of low molecular weight of peptide by controlling the operating parameters with the suitable configuration membrane. MDPI AG 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/95556/1/Performance%20of%20single%20and%20two-stage%20cross-flow%20ultrafiltration%20membrane%20in%20fractionation%20of%20peptide%20from%20microalgae%20protein%20hydrolysate%20%28nannochloropsis%20gaditana%29.pdf Md Saleh, Nur Izzati and Wan Ab Karim Ghani, Wan Azlina and Mustapa Kamal, Siti Mazlina and Harun, Razif (2021) Performance of single and two-stage cross-flow ultrafiltration membrane in fractionation of peptide from microalgae protein hydrolysate (nannochloropsis gaditana). Processes, 9 (4). art. no. 610. pp. 1-20. ISSN 2227-9717 https://www.mdpi.com/2227-9717/9/4/610/htm 10.3390/pr9040610 |
| spellingShingle | Md Saleh, Nur Izzati Wan Ab Karim Ghani, Wan Azlina Mustapa Kamal, Siti Mazlina Harun, Razif Performance of single and two-stage cross-flow ultrafiltration membrane in fractionation of peptide from microalgae protein hydrolysate (nannochloropsis gaditana) |
| title | Performance of single and two-stage cross-flow ultrafiltration membrane in fractionation of peptide from microalgae protein hydrolysate (nannochloropsis gaditana) |
| title_full | Performance of single and two-stage cross-flow ultrafiltration membrane in fractionation of peptide from microalgae protein hydrolysate (nannochloropsis gaditana) |
| title_fullStr | Performance of single and two-stage cross-flow ultrafiltration membrane in fractionation of peptide from microalgae protein hydrolysate (nannochloropsis gaditana) |
| title_full_unstemmed | Performance of single and two-stage cross-flow ultrafiltration membrane in fractionation of peptide from microalgae protein hydrolysate (nannochloropsis gaditana) |
| title_short | Performance of single and two-stage cross-flow ultrafiltration membrane in fractionation of peptide from microalgae protein hydrolysate (nannochloropsis gaditana) |
| title_sort | performance of single and two stage cross flow ultrafiltration membrane in fractionation of peptide from microalgae protein hydrolysate nannochloropsis gaditana |
| url | http://psasir.upm.edu.my/id/eprint/95556/1/Performance%20of%20single%20and%20two-stage%20cross-flow%20ultrafiltration%20membrane%20in%20fractionation%20of%20peptide%20from%20microalgae%20protein%20hydrolysate%20%28nannochloropsis%20gaditana%29.pdf |
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