Application of kenaf seed oil-nanostructured lipid carrier to palm-based α-tocopherol cream for photoprotection
The application of a nanostructured lipid carrier (NLC) to ultraviolet (UV) filter encapsulation was found to enhance its safe use. In this work, a kenaf seed oil-NLC (KSO-NLC) coloaded with 1% diethylamino hydroxybenzoyl hexyl benzoate and 1% ethylhexyl triazone was used as an active ingredient in...
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| Format: | Article |
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John Wiley & Sons
2021
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| _version_ | 1825937644733857792 |
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| author | Chu, Chee Chin Azizul Hasan, Zafarizal Aldrin Tan, Chin Ping Nyam, Kar Lin |
| author_facet | Chu, Chee Chin Azizul Hasan, Zafarizal Aldrin Tan, Chin Ping Nyam, Kar Lin |
| author_sort | Chu, Chee Chin |
| collection | UPM |
| description | The application of a nanostructured lipid carrier (NLC) to ultraviolet (UV) filter encapsulation was found to enhance its safe use. In this work, a kenaf seed oil-NLC (KSO-NLC) coloaded with 1% diethylamino hydroxybenzoyl hexyl benzoate and 1% ethylhexyl triazone was used as an active ingredient in α-tocopherol cream to develop a photoprotective prototype. It was then subjected to further analysis to determine its physical properties, storage stability, and cytotoxicity. The ratio of KSO-NLC to α-tocopherol cream was optimized based on the sun protection factor (SPF) value using a UV transmittance analyzer. The physical properties of the samples were analyzed, and the amount of α-tocopherol was quantified by ultra-high-performance liquid chromatography. The optimized sample was then evaluated for in vitro antioxidant activities using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid assays. Meanwhile, in vitro cytotoxicity was studied on a normal human dermal fibroblast cell line using thiazolyl blue tetrazolium bromide and cell counting kit-8 assays. The sample of KSO-NLC to α-tocopherol cream in a ratio of 1:2 (N3) showed SPF value >50. Besides, the samples showed microbial stability and sustainable α-tocopherol content upon 12 weeks of storage. The cytotoxicity evaluation of N3 and α-tocopherol cream (N5) showed >100% cell proliferation, which indicated that there is no side effect on the cell growth, while triggering the cell proliferation with the presence of bioactive compounds. Overall, the findings of the study are promising for the development of new sunscreen formulation with the utilization of KSO-NLC and palm-based α-tocopherol cream. |
| first_indexed | 2024-03-06T11:02:39Z |
| format | Article |
| id | upm.eprints-95953 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T11:02:39Z |
| publishDate | 2021 |
| publisher | John Wiley & Sons |
| record_format | dspace |
| spelling | upm.eprints-959532023-03-17T02:09:09Z http://psasir.upm.edu.my/id/eprint/95953/ Application of kenaf seed oil-nanostructured lipid carrier to palm-based α-tocopherol cream for photoprotection Chu, Chee Chin Azizul Hasan, Zafarizal Aldrin Tan, Chin Ping Nyam, Kar Lin The application of a nanostructured lipid carrier (NLC) to ultraviolet (UV) filter encapsulation was found to enhance its safe use. In this work, a kenaf seed oil-NLC (KSO-NLC) coloaded with 1% diethylamino hydroxybenzoyl hexyl benzoate and 1% ethylhexyl triazone was used as an active ingredient in α-tocopherol cream to develop a photoprotective prototype. It was then subjected to further analysis to determine its physical properties, storage stability, and cytotoxicity. The ratio of KSO-NLC to α-tocopherol cream was optimized based on the sun protection factor (SPF) value using a UV transmittance analyzer. The physical properties of the samples were analyzed, and the amount of α-tocopherol was quantified by ultra-high-performance liquid chromatography. The optimized sample was then evaluated for in vitro antioxidant activities using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid assays. Meanwhile, in vitro cytotoxicity was studied on a normal human dermal fibroblast cell line using thiazolyl blue tetrazolium bromide and cell counting kit-8 assays. The sample of KSO-NLC to α-tocopherol cream in a ratio of 1:2 (N3) showed SPF value >50. Besides, the samples showed microbial stability and sustainable α-tocopherol content upon 12 weeks of storage. The cytotoxicity evaluation of N3 and α-tocopherol cream (N5) showed >100% cell proliferation, which indicated that there is no side effect on the cell growth, while triggering the cell proliferation with the presence of bioactive compounds. Overall, the findings of the study are promising for the development of new sunscreen formulation with the utilization of KSO-NLC and palm-based α-tocopherol cream. John Wiley & Sons 2021 Article PeerReviewed Chu, Chee Chin and Azizul Hasan, Zafarizal Aldrin and Tan, Chin Ping and Nyam, Kar Lin (2021) Application of kenaf seed oil-nanostructured lipid carrier to palm-based α-tocopherol cream for photoprotection. Journal of the American Oil Chemists' Society, 98 (2). 201 - 210. ISSN 0003-021X; ESSN: 1558-9331 https://aocs.onlinelibrary.wiley.com/doi/abs/10.1002/aocs.12460 10.1002/aocs.12460 |
| spellingShingle | Chu, Chee Chin Azizul Hasan, Zafarizal Aldrin Tan, Chin Ping Nyam, Kar Lin Application of kenaf seed oil-nanostructured lipid carrier to palm-based α-tocopherol cream for photoprotection |
| title | Application of kenaf seed oil-nanostructured lipid carrier to palm-based α-tocopherol cream for photoprotection |
| title_full | Application of kenaf seed oil-nanostructured lipid carrier to palm-based α-tocopherol cream for photoprotection |
| title_fullStr | Application of kenaf seed oil-nanostructured lipid carrier to palm-based α-tocopherol cream for photoprotection |
| title_full_unstemmed | Application of kenaf seed oil-nanostructured lipid carrier to palm-based α-tocopherol cream for photoprotection |
| title_short | Application of kenaf seed oil-nanostructured lipid carrier to palm-based α-tocopherol cream for photoprotection |
| title_sort | application of kenaf seed oil nanostructured lipid carrier to palm based α tocopherol cream for photoprotection |
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