CaWRKY30 positively regulates pepper immunity by targeting CaWRKY40 against Ralstonia solanacearum inoculation through modulating defense-related genes
The WRKY transcription factors (TFs) network is composed of WRKY TFs’ subset, which performs a critical role in immunity regulation of plants. However, functions of WRKY TFs’ network remain unclear, particularly in non-model plants such as pepper (Capsicum annuum L.). This study functionally charact...
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2021
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| _version_ | 1825937691653439488 |
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| author | Hussain, Ansar Khan, Muhammad Ifnan Albaqami, Mohammed Mahpara, Shahzadi Noorka, Ijaz Rasool Ahmed, Mohamed A. A. Aljuaid, Bandar S. El-Shehawi, Ahmed M. Liu, Zhiqin Farooq, Shahid Tan, Ali Kee Zuan |
| author_facet | Hussain, Ansar Khan, Muhammad Ifnan Albaqami, Mohammed Mahpara, Shahzadi Noorka, Ijaz Rasool Ahmed, Mohamed A. A. Aljuaid, Bandar S. El-Shehawi, Ahmed M. Liu, Zhiqin Farooq, Shahid Tan, Ali Kee Zuan |
| author_sort | Hussain, Ansar |
| collection | UPM |
| description | The WRKY transcription factors (TFs) network is composed of WRKY TFs’ subset, which performs a critical role in immunity regulation of plants. However, functions of WRKY TFs’ network remain unclear, particularly in non-model plants such as pepper (Capsicum annuum L.). This study functionally characterized CaWRKY30—a member of group III Pepper WRKY protein—for immunity of pepper against Ralstonia solanacearum infection. The CaWRKY30 was detected in nucleus, and its transcriptional expression levels were significantly upregulated by R. solanacearum inoculation (RSI), and foliar application ethylene (ET), abscisic acid (ABA), and salicylic acid (SA). Virus induced gene silencing (VIGS) of CaWRKY30 amplified pepper’s vulnerability to RSI. Additionally, the silencing of CaWRKY30 by VIGS compromised HR-like cell death triggered by RSI and downregulated defense-associated marker genes, like CaPR1, CaNPR1, CaDEF1, CaABR1, CaHIR1, and CaWRKY40. Conversely, transient over-expression of CaWRKY30 in pepper leaves instigated HR-like cell death and upregulated defense-related maker genes. Furthermore, transient over-expression of CaWRKY30 upregulated transcriptional levels of CaWRKY6, CaWRKY22, CaWRKY27, and CaWRKY40. On the other hand, transient over-expression of CaWRKY6, CaWRKY22, CaWRKY27, and CaWRKY40 upregulated transcriptional expression levels of CaWRKY30. The results recommend that newly characterized CaWRKY30 positively regulates pepper’s immunity against Ralstonia attack, which is governed by synergistically mediated signaling by phytohormones like ET, ABA, and SA, and transcriptionally assimilating into WRKY TFs networks, consisting of CaWRKY6, CaWRKY22, CaWRKY27, and CaWRKY40. Collectively, our data will facilitate to explicate the underlying mechanism of crosstalk between pepper’s immunity and response to RSI. |
| first_indexed | 2024-03-06T11:03:22Z |
| format | Article |
| id | upm.eprints-96306 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T11:03:22Z |
| publishDate | 2021 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | upm.eprints-963062023-01-31T01:58:29Z http://psasir.upm.edu.my/id/eprint/96306/ CaWRKY30 positively regulates pepper immunity by targeting CaWRKY40 against Ralstonia solanacearum inoculation through modulating defense-related genes Hussain, Ansar Khan, Muhammad Ifnan Albaqami, Mohammed Mahpara, Shahzadi Noorka, Ijaz Rasool Ahmed, Mohamed A. A. Aljuaid, Bandar S. El-Shehawi, Ahmed M. Liu, Zhiqin Farooq, Shahid Tan, Ali Kee Zuan The WRKY transcription factors (TFs) network is composed of WRKY TFs’ subset, which performs a critical role in immunity regulation of plants. However, functions of WRKY TFs’ network remain unclear, particularly in non-model plants such as pepper (Capsicum annuum L.). This study functionally characterized CaWRKY30—a member of group III Pepper WRKY protein—for immunity of pepper against Ralstonia solanacearum infection. The CaWRKY30 was detected in nucleus, and its transcriptional expression levels were significantly upregulated by R. solanacearum inoculation (RSI), and foliar application ethylene (ET), abscisic acid (ABA), and salicylic acid (SA). Virus induced gene silencing (VIGS) of CaWRKY30 amplified pepper’s vulnerability to RSI. Additionally, the silencing of CaWRKY30 by VIGS compromised HR-like cell death triggered by RSI and downregulated defense-associated marker genes, like CaPR1, CaNPR1, CaDEF1, CaABR1, CaHIR1, and CaWRKY40. Conversely, transient over-expression of CaWRKY30 in pepper leaves instigated HR-like cell death and upregulated defense-related maker genes. Furthermore, transient over-expression of CaWRKY30 upregulated transcriptional levels of CaWRKY6, CaWRKY22, CaWRKY27, and CaWRKY40. On the other hand, transient over-expression of CaWRKY6, CaWRKY22, CaWRKY27, and CaWRKY40 upregulated transcriptional expression levels of CaWRKY30. The results recommend that newly characterized CaWRKY30 positively regulates pepper’s immunity against Ralstonia attack, which is governed by synergistically mediated signaling by phytohormones like ET, ABA, and SA, and transcriptionally assimilating into WRKY TFs networks, consisting of CaWRKY6, CaWRKY22, CaWRKY27, and CaWRKY40. Collectively, our data will facilitate to explicate the underlying mechanism of crosstalk between pepper’s immunity and response to RSI. Multidisciplinary Digital Publishing Institute 2021 Article PeerReviewed Hussain, Ansar and Khan, Muhammad Ifnan and Albaqami, Mohammed and Mahpara, Shahzadi and Noorka, Ijaz Rasool and Ahmed, Mohamed A. A. and Aljuaid, Bandar S. and El-Shehawi, Ahmed M. and Liu, Zhiqin and Farooq, Shahid and Tan, Ali Kee Zuan (2021) CaWRKY30 positively regulates pepper immunity by targeting CaWRKY40 against Ralstonia solanacearum inoculation through modulating defense-related genes. International Journal of Molecular Sciences, 22 (21). art. no. 12091. pp. 1-18. ISSN 1661-6596; ESSN: 1422-0067 https://www.mdpi.com/1422-0067/22/21/12091 10.3390/ijms222112091 |
| spellingShingle | Hussain, Ansar Khan, Muhammad Ifnan Albaqami, Mohammed Mahpara, Shahzadi Noorka, Ijaz Rasool Ahmed, Mohamed A. A. Aljuaid, Bandar S. El-Shehawi, Ahmed M. Liu, Zhiqin Farooq, Shahid Tan, Ali Kee Zuan CaWRKY30 positively regulates pepper immunity by targeting CaWRKY40 against Ralstonia solanacearum inoculation through modulating defense-related genes |
| title | CaWRKY30 positively regulates pepper immunity by targeting CaWRKY40 against Ralstonia solanacearum inoculation through modulating defense-related genes |
| title_full | CaWRKY30 positively regulates pepper immunity by targeting CaWRKY40 against Ralstonia solanacearum inoculation through modulating defense-related genes |
| title_fullStr | CaWRKY30 positively regulates pepper immunity by targeting CaWRKY40 against Ralstonia solanacearum inoculation through modulating defense-related genes |
| title_full_unstemmed | CaWRKY30 positively regulates pepper immunity by targeting CaWRKY40 against Ralstonia solanacearum inoculation through modulating defense-related genes |
| title_short | CaWRKY30 positively regulates pepper immunity by targeting CaWRKY40 against Ralstonia solanacearum inoculation through modulating defense-related genes |
| title_sort | cawrky30 positively regulates pepper immunity by targeting cawrky40 against ralstonia solanacearum inoculation through modulating defense related genes |
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