Characterization and extraction influence protein profiling of edible bird’s nest
The edible bird nest (EBN) from Aerodramus fuciphagus has been consumed as a Chinese traditional food for health and medicinal purposes due to its elevated nutritional value. The present study focused on the influence of characterization and extraction methods on protein profiling, which could be a...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Published: |
Multidisciplinary Digital Publishing Institute
2021
|
| _version_ | 1825937698680995840 |
|---|---|
| author | Mohamad Nasir, Nurul Nadiah Mohamad Ibrahim, Ramlah Abu Bakar @ Zakaria, Md Zuki Mahmud, Rozi Ab Razak, Nor Asma |
| author_facet | Mohamad Nasir, Nurul Nadiah Mohamad Ibrahim, Ramlah Abu Bakar @ Zakaria, Md Zuki Mahmud, Rozi Ab Razak, Nor Asma |
| author_sort | Mohamad Nasir, Nurul Nadiah |
| collection | UPM |
| description | The edible bird nest (EBN) from Aerodramus fuciphagus has been consumed as a Chinese traditional food for health and medicinal purposes due to its elevated nutritional value. The present study focused on the influence of characterization and extraction methods on protein profiling, which could be a guideline for grading the EBN. The proposed extraction method is similar to the common food preparation methods of consumers and thus can accurately establish the bioactive protein available upon human consumption. The characterization includes physicochemical analysis (physical, morphology, elemental composition, and microbial content) and chemical analysis (crude protein and amino acid). The morphology of half-cup EBN was found to be uniformly shaped and rich in calcium as compared to rough surface of stripe-shaped EBN, and there was no significant microbial growth in both types of EBN. The crude protein and amino acid content in half-cup EBN were significantly higher than stripe-shaped EBN. The full stew (FS) and stew (SE) extraction methods produced a maximal yield of soluble protein. Sialic acid content in SE extract (8.47%, w/w) and FS extract (7.91%, w/w) were recorded. About seven parent proteins (39.15 to 181.68 kDa) were identified by LC-MS/MS Q-TOF, namely 78 kDa glucose-regulated protein, lysyl oxidase-3, Mucin-5AC-like, acidic mammalian chitinase-like, 45 kDa calcium-binding protein, nucleobindin-2, and ovoinhibitor-like. In conclusion, the characteristics and extraction methods influence the availability of bioactive protein and peptides, demonstrating the potential usage of EBN in improving its biological activities and nutritional properties. |
| first_indexed | 2024-03-06T11:03:28Z |
| format | Article |
| id | upm.eprints-96351 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T11:03:28Z |
| publishDate | 2021 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | upm.eprints-963512023-01-31T02:02:00Z http://psasir.upm.edu.my/id/eprint/96351/ Characterization and extraction influence protein profiling of edible bird’s nest Mohamad Nasir, Nurul Nadiah Mohamad Ibrahim, Ramlah Abu Bakar @ Zakaria, Md Zuki Mahmud, Rozi Ab Razak, Nor Asma The edible bird nest (EBN) from Aerodramus fuciphagus has been consumed as a Chinese traditional food for health and medicinal purposes due to its elevated nutritional value. The present study focused on the influence of characterization and extraction methods on protein profiling, which could be a guideline for grading the EBN. The proposed extraction method is similar to the common food preparation methods of consumers and thus can accurately establish the bioactive protein available upon human consumption. The characterization includes physicochemical analysis (physical, morphology, elemental composition, and microbial content) and chemical analysis (crude protein and amino acid). The morphology of half-cup EBN was found to be uniformly shaped and rich in calcium as compared to rough surface of stripe-shaped EBN, and there was no significant microbial growth in both types of EBN. The crude protein and amino acid content in half-cup EBN were significantly higher than stripe-shaped EBN. The full stew (FS) and stew (SE) extraction methods produced a maximal yield of soluble protein. Sialic acid content in SE extract (8.47%, w/w) and FS extract (7.91%, w/w) were recorded. About seven parent proteins (39.15 to 181.68 kDa) were identified by LC-MS/MS Q-TOF, namely 78 kDa glucose-regulated protein, lysyl oxidase-3, Mucin-5AC-like, acidic mammalian chitinase-like, 45 kDa calcium-binding protein, nucleobindin-2, and ovoinhibitor-like. In conclusion, the characteristics and extraction methods influence the availability of bioactive protein and peptides, demonstrating the potential usage of EBN in improving its biological activities and nutritional properties. Multidisciplinary Digital Publishing Institute 2021 Article PeerReviewed Mohamad Nasir, Nurul Nadiah and Mohamad Ibrahim, Ramlah and Abu Bakar @ Zakaria, Md Zuki and Mahmud, Rozi and Ab Razak, Nor Asma (2021) Characterization and extraction influence protein profiling of edible bird’s nest. Foods, 10 (10). art. no. 2248. pp. 1-20. ISSN 2304-8158 https://www.mdpi.com/2304-8158/10/10/2248 10.3390/foods10102248 |
| spellingShingle | Mohamad Nasir, Nurul Nadiah Mohamad Ibrahim, Ramlah Abu Bakar @ Zakaria, Md Zuki Mahmud, Rozi Ab Razak, Nor Asma Characterization and extraction influence protein profiling of edible bird’s nest |
| title | Characterization and extraction influence protein profiling of edible bird’s nest |
| title_full | Characterization and extraction influence protein profiling of edible bird’s nest |
| title_fullStr | Characterization and extraction influence protein profiling of edible bird’s nest |
| title_full_unstemmed | Characterization and extraction influence protein profiling of edible bird’s nest |
| title_short | Characterization and extraction influence protein profiling of edible bird’s nest |
| title_sort | characterization and extraction influence protein profiling of edible bird s nest |
| work_keys_str_mv | AT mohamadnasirnurulnadiah characterizationandextractioninfluenceproteinprofilingofediblebirdsnest AT mohamadibrahimramlah characterizationandextractioninfluenceproteinprofilingofediblebirdsnest AT abubakarzakariamdzuki characterizationandextractioninfluenceproteinprofilingofediblebirdsnest AT mahmudrozi characterizationandextractioninfluenceproteinprofilingofediblebirdsnest AT abrazaknorasma characterizationandextractioninfluenceproteinprofilingofediblebirdsnest |