Combination of molecular networking and LC-MS/MS profiling in investigating the interrelationships between the antioxidant and antimicrobial properties of Curculigo latifolia
Curculigo is a potent plant with a variety of traditional uses, such as anti-oxidant, anti-diabetic, anti-tumor, anti-bacterial, anti-cancer, anti-osteoporosis, and wound-healing. The comprehensive profiling of the Curculigolatifolia metabolome was carried out by generating a molecular network (MN)...
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2021
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| author | Mad Nasir, Nadiah Ezam Shah, Nur Syafiqah Zainal, Nurul Zulaikha Kassim, Nur Kartinee Mohd Faudzi, Siti Munirah Hasan, Hanan |
| author_facet | Mad Nasir, Nadiah Ezam Shah, Nur Syafiqah Zainal, Nurul Zulaikha Kassim, Nur Kartinee Mohd Faudzi, Siti Munirah Hasan, Hanan |
| author_sort | Mad Nasir, Nadiah |
| collection | UPM |
| description | Curculigo is a potent plant with a variety of traditional uses, such as anti-oxidant, anti-diabetic, anti-tumor, anti-bacterial, anti-cancer, anti-osteoporosis, and wound-healing. The comprehensive profiling of the Curculigolatifolia metabolome was carried out by generating a molecular network (MN) from Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS) data to profile the methanol extract and correlating them with their antioxidant (2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), total phenolic contents (TPC), and β-carotene) and antimicrobial (disk-diffusion agar method, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC)) properties. The antioxidant capacity was observed to be significantly higher in the rhizome crude extract, with 18.10 ± 0.91 µg/mL DPPH activity, and a β-carotene bleaching result of 35.20%. For the antimicrobial activity, the leaf crude extract exhibited a strong Staphylococcus aureus and Salmonella choleraesuis (8–15 ± 3.0 mm) inhibition in the disk-diffusion agar. The leaf extract also exhibited maximum antibacterial activity against S. aureus (MIC = ±0.25 mg/mL, MBC = ±0.25 mg/mL) and S. choleraesuis (MIC = ±0.25 mg/mL, MBC = ±0.25 mg/mL). LC-MS/MS analysis and MN revealed norlignans and phenolic glycosides as major metabolites in the rhizome and leaf extracts of the negative mode (M − H)−. Fourteen known compounds were identified, and three unknown compounds were putatively identified in the rhizome extract, while ten known compounds and six unknown compounds were putatively identified in the leaf extract. |
| first_indexed | 2024-03-06T11:03:41Z |
| format | Article |
| id | upm.eprints-96435 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T11:03:41Z |
| publishDate | 2021 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | upm.eprints-964352023-01-26T02:26:35Z http://psasir.upm.edu.my/id/eprint/96435/ Combination of molecular networking and LC-MS/MS profiling in investigating the interrelationships between the antioxidant and antimicrobial properties of Curculigo latifolia Mad Nasir, Nadiah Ezam Shah, Nur Syafiqah Zainal, Nurul Zulaikha Kassim, Nur Kartinee Mohd Faudzi, Siti Munirah Hasan, Hanan Curculigo is a potent plant with a variety of traditional uses, such as anti-oxidant, anti-diabetic, anti-tumor, anti-bacterial, anti-cancer, anti-osteoporosis, and wound-healing. The comprehensive profiling of the Curculigolatifolia metabolome was carried out by generating a molecular network (MN) from Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS) data to profile the methanol extract and correlating them with their antioxidant (2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), total phenolic contents (TPC), and β-carotene) and antimicrobial (disk-diffusion agar method, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC)) properties. The antioxidant capacity was observed to be significantly higher in the rhizome crude extract, with 18.10 ± 0.91 µg/mL DPPH activity, and a β-carotene bleaching result of 35.20%. For the antimicrobial activity, the leaf crude extract exhibited a strong Staphylococcus aureus and Salmonella choleraesuis (8–15 ± 3.0 mm) inhibition in the disk-diffusion agar. The leaf extract also exhibited maximum antibacterial activity against S. aureus (MIC = ±0.25 mg/mL, MBC = ±0.25 mg/mL) and S. choleraesuis (MIC = ±0.25 mg/mL, MBC = ±0.25 mg/mL). LC-MS/MS analysis and MN revealed norlignans and phenolic glycosides as major metabolites in the rhizome and leaf extracts of the negative mode (M − H)−. Fourteen known compounds were identified, and three unknown compounds were putatively identified in the rhizome extract, while ten known compounds and six unknown compounds were putatively identified in the leaf extract. Multidisciplinary Digital Publishing Institute 2021 Article PeerReviewed Mad Nasir, Nadiah and Ezam Shah, Nur Syafiqah and Zainal, Nurul Zulaikha and Kassim, Nur Kartinee and Mohd Faudzi, Siti Munirah and Hasan, Hanan (2021) Combination of molecular networking and LC-MS/MS profiling in investigating the interrelationships between the antioxidant and antimicrobial properties of Curculigo latifolia. Plants-Basel, 10 (8). art. no. 1488. pp. 1-18. ISSN 2223-7747 https://www.mdpi.com/2223-7747/10/8/1488 10.3390/plants10081488 |
| spellingShingle | Mad Nasir, Nadiah Ezam Shah, Nur Syafiqah Zainal, Nurul Zulaikha Kassim, Nur Kartinee Mohd Faudzi, Siti Munirah Hasan, Hanan Combination of molecular networking and LC-MS/MS profiling in investigating the interrelationships between the antioxidant and antimicrobial properties of Curculigo latifolia |
| title | Combination of molecular networking and LC-MS/MS profiling in investigating the interrelationships between the antioxidant and antimicrobial properties of Curculigo latifolia |
| title_full | Combination of molecular networking and LC-MS/MS profiling in investigating the interrelationships between the antioxidant and antimicrobial properties of Curculigo latifolia |
| title_fullStr | Combination of molecular networking and LC-MS/MS profiling in investigating the interrelationships between the antioxidant and antimicrobial properties of Curculigo latifolia |
| title_full_unstemmed | Combination of molecular networking and LC-MS/MS profiling in investigating the interrelationships between the antioxidant and antimicrobial properties of Curculigo latifolia |
| title_short | Combination of molecular networking and LC-MS/MS profiling in investigating the interrelationships between the antioxidant and antimicrobial properties of Curculigo latifolia |
| title_sort | combination of molecular networking and lc ms ms profiling in investigating the interrelationships between the antioxidant and antimicrobial properties of curculigo latifolia |
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