Comparative study of the yield and physicochemical properties of collagen from Sea Cucumber (Holothuria scabra), Obtained through Dialysis and the Ultrafiltration Membrane
Collagen was extracted from the body wall of sea cucumber (Holothuria scabra) using the pepsin-solubilized collagen method followed by isolation using dialysis and the ultrafiltration membrane. The yield and physicochemical properties of the collagen obtained from both isolation methods, denoted as...
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2021
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| _version_ | 1796983242926063616 |
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| author | Saallah, Suryani Roslan, Jumardi Julius, Flavian Sheryl Saallah, Sharinee Mohamad Razali, Umi Hartina Pindi, Wolyna Sulaiman, Mohd Rosni Pa’ee, Khairul Faizal Mustapa Kamal, Siti Mazlina |
| author_facet | Saallah, Suryani Roslan, Jumardi Julius, Flavian Sheryl Saallah, Sharinee Mohamad Razali, Umi Hartina Pindi, Wolyna Sulaiman, Mohd Rosni Pa’ee, Khairul Faizal Mustapa Kamal, Siti Mazlina |
| author_sort | Saallah, Suryani |
| collection | UPM |
| description | Collagen was extracted from the body wall of sea cucumber (Holothuria scabra) using the pepsin-solubilized collagen method followed by isolation using dialysis and the ultrafiltration membrane. The yield and physicochemical properties of the collagen obtained from both isolation methods, denoted as D-PSC and UF-PSC, were compared. The ultrafiltration method affords a higher yield of collagen (11.39%) than that of the dialysis (5.15%). The isolated collagens have almost the same amino acid composition, while their functional groups, referred to as amide A, B, I, II, and III bands, were in accordance with commercial collagen, as verified by Fourier Transform Infrared (FT-IR) spectroscopy. The UV-Vis absorption peaks at 240 nm and 220 nm, respectively, indicated that the collagens produced are type-I collagen. The D-PSC showed interconnecting sheet-like fibrils, while the UF-PSC exhibited a flaky structure with flat-sheets arranged very close to each other. The higher yield and comparable physicochemical properties of the collagen obtained by ultrafiltration as compared with dialysis indicate that the membrane process has high potential to be used in large-scale collagen production for food and pharmaceutical applications. |
| first_indexed | 2024-03-06T11:03:44Z |
| format | Article |
| id | upm.eprints-96458 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T11:03:44Z |
| publishDate | 2021 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | upm.eprints-964582023-01-11T09:03:32Z http://psasir.upm.edu.my/id/eprint/96458/ Comparative study of the yield and physicochemical properties of collagen from Sea Cucumber (Holothuria scabra), Obtained through Dialysis and the Ultrafiltration Membrane Saallah, Suryani Roslan, Jumardi Julius, Flavian Sheryl Saallah, Sharinee Mohamad Razali, Umi Hartina Pindi, Wolyna Sulaiman, Mohd Rosni Pa’ee, Khairul Faizal Mustapa Kamal, Siti Mazlina Collagen was extracted from the body wall of sea cucumber (Holothuria scabra) using the pepsin-solubilized collagen method followed by isolation using dialysis and the ultrafiltration membrane. The yield and physicochemical properties of the collagen obtained from both isolation methods, denoted as D-PSC and UF-PSC, were compared. The ultrafiltration method affords a higher yield of collagen (11.39%) than that of the dialysis (5.15%). The isolated collagens have almost the same amino acid composition, while their functional groups, referred to as amide A, B, I, II, and III bands, were in accordance with commercial collagen, as verified by Fourier Transform Infrared (FT-IR) spectroscopy. The UV-Vis absorption peaks at 240 nm and 220 nm, respectively, indicated that the collagens produced are type-I collagen. The D-PSC showed interconnecting sheet-like fibrils, while the UF-PSC exhibited a flaky structure with flat-sheets arranged very close to each other. The higher yield and comparable physicochemical properties of the collagen obtained by ultrafiltration as compared with dialysis indicate that the membrane process has high potential to be used in large-scale collagen production for food and pharmaceutical applications. Multidisciplinary Digital Publishing Institute 2021 Article PeerReviewed Saallah, Suryani and Roslan, Jumardi and Julius, Flavian Sheryl and Saallah, Sharinee and Mohamad Razali, Umi Hartina and Pindi, Wolyna and Sulaiman, Mohd Rosni and Pa’ee, Khairul Faizal and Mustapa Kamal, Siti Mazlina (2021) Comparative study of the yield and physicochemical properties of collagen from Sea Cucumber (Holothuria scabra), Obtained through Dialysis and the Ultrafiltration Membrane. Molecules, 26 (9). art. no. 2564. pp. 1-11. ISSN 1420-3049 https://www.mdpi.com/1420-3049/26/9/2564 10.3390/molecules26092564 |
| spellingShingle | Saallah, Suryani Roslan, Jumardi Julius, Flavian Sheryl Saallah, Sharinee Mohamad Razali, Umi Hartina Pindi, Wolyna Sulaiman, Mohd Rosni Pa’ee, Khairul Faizal Mustapa Kamal, Siti Mazlina Comparative study of the yield and physicochemical properties of collagen from Sea Cucumber (Holothuria scabra), Obtained through Dialysis and the Ultrafiltration Membrane |
| title | Comparative study of the yield and physicochemical properties of collagen from Sea Cucumber (Holothuria scabra), Obtained through Dialysis and the Ultrafiltration Membrane |
| title_full | Comparative study of the yield and physicochemical properties of collagen from Sea Cucumber (Holothuria scabra), Obtained through Dialysis and the Ultrafiltration Membrane |
| title_fullStr | Comparative study of the yield and physicochemical properties of collagen from Sea Cucumber (Holothuria scabra), Obtained through Dialysis and the Ultrafiltration Membrane |
| title_full_unstemmed | Comparative study of the yield and physicochemical properties of collagen from Sea Cucumber (Holothuria scabra), Obtained through Dialysis and the Ultrafiltration Membrane |
| title_short | Comparative study of the yield and physicochemical properties of collagen from Sea Cucumber (Holothuria scabra), Obtained through Dialysis and the Ultrafiltration Membrane |
| title_sort | comparative study of the yield and physicochemical properties of collagen from sea cucumber holothuria scabra obtained through dialysis and the ultrafiltration membrane |
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