Diarylpentanoid (1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one) (MS13) exhibits anti-proliferative, apoptosis induction and anti-migration properties on androgen-independent human prostate cancer by targeting cell cycle-apoptosis and PI3K signalling pathways
Diarylpentanoids exhibit a high degree of anti-cancer activity and stability in vitro over curcumin in prostate cancer cells. Hence, this study aims to investigate the effects of a diarylpentanoid, 1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one (MS13) on cytotoxicity, anti-proliferative, ap...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/96718/1/ABSTRACT.pdf |
| _version_ | 1825937754170589184 |
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| author | Abd Wahab, Nurul Azwa Abas, Faridah Othman, Iekhsan Naidu, Rakesh |
| author_facet | Abd Wahab, Nurul Azwa Abas, Faridah Othman, Iekhsan Naidu, Rakesh |
| author_sort | Abd Wahab, Nurul Azwa |
| collection | UPM |
| description | Diarylpentanoids exhibit a high degree of anti-cancer activity and stability in vitro over curcumin in prostate cancer cells. Hence, this study aims to investigate the effects of a diarylpentanoid, 1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one (MS13) on cytotoxicity, anti-proliferative, apoptosis-inducing, anti-migration properties, and the underlying molecular mechanisms on treated androgen-independent prostate cancer cells, DU 145 and PC-3. A cell viability assay has shown greater cytotoxicity effects of MS13-treated DU 145 cells (EC50 7.57 ± 0.2 µM) and PC-3 cells (EC50 7.80 ± 0.7 µM) compared to curcumin (EC50: DU 145; 34.25 ± 2.7 µM and PC-3; 27.77 ± 6.4 µM). In addition, MS13 exhibited significant anti-proliferative activity against AIPC cells compared to curcumin in a dose- and time-dependent manner. Morphological observation, increased caspase-3 activity, and reduced Bcl-2 protein levels in these cells indicated that MS13 induces apoptosis in a time- and dose-dependent. Moreover, MS13 effectively inhibited the migration of DU 145 and PC-3 cells. Our results suggest that cell cycle-apoptosis and PI3K pathways were the topmost significant pathways impacted by MS13 activity. Our findings suggest that MS13 may demonstrate the anti-cancer activity by modulating DEGs associated with the cell cycle-apoptosis and PI3K pathways, thus inhibiting cell proliferation and cell migration as well as inducing apoptosis in AIPC cells. |
| first_indexed | 2024-03-06T11:04:19Z |
| format | Article |
| id | upm.eprints-96718 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T11:04:19Z |
| publishDate | 2021 |
| publisher | Frontiers Media |
| record_format | dspace |
| spelling | upm.eprints-967182022-12-01T08:11:05Z http://psasir.upm.edu.my/id/eprint/96718/ Diarylpentanoid (1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one) (MS13) exhibits anti-proliferative, apoptosis induction and anti-migration properties on androgen-independent human prostate cancer by targeting cell cycle-apoptosis and PI3K signalling pathways Abd Wahab, Nurul Azwa Abas, Faridah Othman, Iekhsan Naidu, Rakesh Diarylpentanoids exhibit a high degree of anti-cancer activity and stability in vitro over curcumin in prostate cancer cells. Hence, this study aims to investigate the effects of a diarylpentanoid, 1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one (MS13) on cytotoxicity, anti-proliferative, apoptosis-inducing, anti-migration properties, and the underlying molecular mechanisms on treated androgen-independent prostate cancer cells, DU 145 and PC-3. A cell viability assay has shown greater cytotoxicity effects of MS13-treated DU 145 cells (EC50 7.57 ± 0.2 µM) and PC-3 cells (EC50 7.80 ± 0.7 µM) compared to curcumin (EC50: DU 145; 34.25 ± 2.7 µM and PC-3; 27.77 ± 6.4 µM). In addition, MS13 exhibited significant anti-proliferative activity against AIPC cells compared to curcumin in a dose- and time-dependent manner. Morphological observation, increased caspase-3 activity, and reduced Bcl-2 protein levels in these cells indicated that MS13 induces apoptosis in a time- and dose-dependent. Moreover, MS13 effectively inhibited the migration of DU 145 and PC-3 cells. Our results suggest that cell cycle-apoptosis and PI3K pathways were the topmost significant pathways impacted by MS13 activity. Our findings suggest that MS13 may demonstrate the anti-cancer activity by modulating DEGs associated with the cell cycle-apoptosis and PI3K pathways, thus inhibiting cell proliferation and cell migration as well as inducing apoptosis in AIPC cells. Frontiers Media 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/96718/1/ABSTRACT.pdf Abd Wahab, Nurul Azwa and Abas, Faridah and Othman, Iekhsan and Naidu, Rakesh (2021) Diarylpentanoid (1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one) (MS13) exhibits anti-proliferative, apoptosis induction and anti-migration properties on androgen-independent human prostate cancer by targeting cell cycle-apoptosis and PI3K signalling pathways. Frontiers in Pharmacology, 12. art. no. 707335. pp. 1-22. ISSN 1663-9812 https://www.frontiersin.org/articles/10.3389/fphar.2021.707335/full 10.3389/fphar.2021.707335 |
| spellingShingle | Abd Wahab, Nurul Azwa Abas, Faridah Othman, Iekhsan Naidu, Rakesh Diarylpentanoid (1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one) (MS13) exhibits anti-proliferative, apoptosis induction and anti-migration properties on androgen-independent human prostate cancer by targeting cell cycle-apoptosis and PI3K signalling pathways |
| title | Diarylpentanoid (1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one) (MS13) exhibits anti-proliferative, apoptosis induction and anti-migration properties on androgen-independent human prostate cancer by targeting cell cycle-apoptosis and PI3K signalling pathways |
| title_full | Diarylpentanoid (1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one) (MS13) exhibits anti-proliferative, apoptosis induction and anti-migration properties on androgen-independent human prostate cancer by targeting cell cycle-apoptosis and PI3K signalling pathways |
| title_fullStr | Diarylpentanoid (1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one) (MS13) exhibits anti-proliferative, apoptosis induction and anti-migration properties on androgen-independent human prostate cancer by targeting cell cycle-apoptosis and PI3K signalling pathways |
| title_full_unstemmed | Diarylpentanoid (1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one) (MS13) exhibits anti-proliferative, apoptosis induction and anti-migration properties on androgen-independent human prostate cancer by targeting cell cycle-apoptosis and PI3K signalling pathways |
| title_short | Diarylpentanoid (1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one) (MS13) exhibits anti-proliferative, apoptosis induction and anti-migration properties on androgen-independent human prostate cancer by targeting cell cycle-apoptosis and PI3K signalling pathways |
| title_sort | diarylpentanoid 1 5 bis 4 hydroxy 3 methoxyphenyl 1 4 pentadiene 3 one ms13 exhibits anti proliferative apoptosis induction and anti migration properties on androgen independent human prostate cancer by targeting cell cycle apoptosis and pi3k signalling pathways |
| url | http://psasir.upm.edu.my/id/eprint/96718/1/ABSTRACT.pdf |
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