Direct shoot organogenesis and clonal fidelity confirmation of Tongkat Ali (Eurycoma longifolia) using molecular markers
Eurycoma longifolia is a medicinally potent plant found in the tropical forest of South-East Asia. Every part of the plant, especially the root is traditionally used as an aphrodisiac, anticancer and anti-inflammatory. E. longifolia is conventionally propagated by seeds but with low germination rate...
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| Format: | Article |
| Language: | English |
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Friends Science Publishers
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/96747/1/ABSTRACT.pdf |
| _version_ | 1825937758811586560 |
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| author | Alttaher, Annor Gebril Annour Balia Yusof, Zetty Norhana Shaharuddin, Noor Azmi |
| author_facet | Alttaher, Annor Gebril Annour Balia Yusof, Zetty Norhana Shaharuddin, Noor Azmi |
| author_sort | Alttaher, Annor Gebril Annour |
| collection | UPM |
| description | Eurycoma longifolia is a medicinally potent plant found in the tropical forest of South-East Asia. Every part of the plant, especially the root is traditionally used as an aphrodisiac, anticancer and anti-inflammatory. E. longifolia is conventionally propagated by seeds but with low germination rate and efficiency. This has made an in vitro propagation of E. longifolia a desirable alternative. Hence, this study reports an effective method of direct organogenesis of shoot. In vitro seedling’s leaves were cultured on Murashige and Skoog (MS) medium containing 1.0 mg L-1 6-benzylaminopurine (BAP), producing 1.8 ± 0.5 shoots per leaf with a regeneration frequency of 68.2%. The shoot buds were directly formed from leaves without intermediate callus formation. To obtain complete plantlets, the shoots were in vitro rooted with an average number of 4.2 ± 0.4 roots per shoot in half-strength MS (½MS) medium supplemented with 0.5 mg L-1 indole-3-butyric acid (IBA). Regenerated plantlets were successfully acclimatized to field conditions with an 85% survival rate. Genetic fidelity of the micropropagated plantlets was evaluated using Simple Sequence Repeat (SSR) and Inter Simple Sequence Repeat (ISSR) analysis. The results showed that the monomorphic banding patterns of in vitro raised plantlets and their mother plant were similar, confirming its homogeneity and the reliability of the multiplication system. |
| first_indexed | 2024-03-06T11:04:24Z |
| format | Article |
| id | upm.eprints-96747 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T11:04:24Z |
| publishDate | 2021 |
| publisher | Friends Science Publishers |
| record_format | dspace |
| spelling | upm.eprints-967472022-12-01T04:34:25Z http://psasir.upm.edu.my/id/eprint/96747/ Direct shoot organogenesis and clonal fidelity confirmation of Tongkat Ali (Eurycoma longifolia) using molecular markers Alttaher, Annor Gebril Annour Balia Yusof, Zetty Norhana Shaharuddin, Noor Azmi Eurycoma longifolia is a medicinally potent plant found in the tropical forest of South-East Asia. Every part of the plant, especially the root is traditionally used as an aphrodisiac, anticancer and anti-inflammatory. E. longifolia is conventionally propagated by seeds but with low germination rate and efficiency. This has made an in vitro propagation of E. longifolia a desirable alternative. Hence, this study reports an effective method of direct organogenesis of shoot. In vitro seedling’s leaves were cultured on Murashige and Skoog (MS) medium containing 1.0 mg L-1 6-benzylaminopurine (BAP), producing 1.8 ± 0.5 shoots per leaf with a regeneration frequency of 68.2%. The shoot buds were directly formed from leaves without intermediate callus formation. To obtain complete plantlets, the shoots were in vitro rooted with an average number of 4.2 ± 0.4 roots per shoot in half-strength MS (½MS) medium supplemented with 0.5 mg L-1 indole-3-butyric acid (IBA). Regenerated plantlets were successfully acclimatized to field conditions with an 85% survival rate. Genetic fidelity of the micropropagated plantlets was evaluated using Simple Sequence Repeat (SSR) and Inter Simple Sequence Repeat (ISSR) analysis. The results showed that the monomorphic banding patterns of in vitro raised plantlets and their mother plant were similar, confirming its homogeneity and the reliability of the multiplication system. Friends Science Publishers 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/96747/1/ABSTRACT.pdf Alttaher, Annor Gebril Annour and Balia Yusof, Zetty Norhana and Shaharuddin, Noor Azmi (2021) Direct shoot organogenesis and clonal fidelity confirmation of Tongkat Ali (Eurycoma longifolia) using molecular markers. International Journal of Agriculture and Biology, 26. pp. 9-16. ISSN 1560-8530; ESSN: 1814-9596 https://www.fspublishers.org/html_issue.php?i_id=41624 10.17957/IJAB/15.1802 |
| spellingShingle | Alttaher, Annor Gebril Annour Balia Yusof, Zetty Norhana Shaharuddin, Noor Azmi Direct shoot organogenesis and clonal fidelity confirmation of Tongkat Ali (Eurycoma longifolia) using molecular markers |
| title | Direct shoot organogenesis and clonal fidelity confirmation of Tongkat Ali (Eurycoma longifolia) using molecular markers |
| title_full | Direct shoot organogenesis and clonal fidelity confirmation of Tongkat Ali (Eurycoma longifolia) using molecular markers |
| title_fullStr | Direct shoot organogenesis and clonal fidelity confirmation of Tongkat Ali (Eurycoma longifolia) using molecular markers |
| title_full_unstemmed | Direct shoot organogenesis and clonal fidelity confirmation of Tongkat Ali (Eurycoma longifolia) using molecular markers |
| title_short | Direct shoot organogenesis and clonal fidelity confirmation of Tongkat Ali (Eurycoma longifolia) using molecular markers |
| title_sort | direct shoot organogenesis and clonal fidelity confirmation of tongkat ali eurycoma longifolia using molecular markers |
| url | http://psasir.upm.edu.my/id/eprint/96747/1/ABSTRACT.pdf |
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