Discovery of known and novel viruses in wild and cultivated blueberry in Florida through viral metagenomic approaches
Southern highbush blueberry (interspecific hybrids of Vaccinium corymbosum L.) is cultivated near wild V. corymbosum as well as closely related species in Florida, USA. The expansion of blueberry cultivation into new areas in Florida and deployment of new cultivars containing viruses can potentially...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Multidisciplinary Digital Publishing Institute
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/96754/1/ABSTRACT.pdf |
| _version_ | 1796983288041046016 |
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| author | Saad, Norsazilawati Olmstead, James W. Varsani, Arvind Polston, Jane Elizabeth Jones, Jeffery Folimonova, Svetlana Y. Harmon, Philip F. |
| author_facet | Saad, Norsazilawati Olmstead, James W. Varsani, Arvind Polston, Jane Elizabeth Jones, Jeffery Folimonova, Svetlana Y. Harmon, Philip F. |
| author_sort | Saad, Norsazilawati |
| collection | UPM |
| description | Southern highbush blueberry (interspecific hybrids of Vaccinium corymbosum L.) is cultivated near wild V. corymbosum as well as closely related species in Florida, USA. The expansion of blueberry cultivation into new areas in Florida and deployment of new cultivars containing viruses can potentially increase the diversity of viruses in wild and cultivated V. corymbosum. In this study, viral diversity in wild and cultivated blueberries (V. corymbosum) is described using a metagenomic approach. RNA viromes from V. corymbosum plants collected from six locations (two cultivated and four wild) in North Central Florida were generated by high throughput sequencing (HTS) and analyzed using a bioinformatic analysis pipeline. De novo assembled contigs obtained from viromes of both commercial and wild sites produced sequences with similarities to plant virus species from a diverse range of families (Amalgaviridae, Caulimoviridae, Endornaviridae, Ophioviridae, Phenuiviridae, and Virgaviridae). In addition, this study has enabled the identification of blueberry latent virus (BlLV) and blueberry mosaic associated ophiovirus (BlMaV) for the first time in Florida, as well as a tentative novel tepovirus (blueberry virus T) (BlVT) in blueberry. To the best of our knowledge, this is the first study that compares viral diversity in wild and cultivated blueberry using a metagenomic approach. |
| first_indexed | 2024-03-06T11:04:24Z |
| format | Article |
| id | upm.eprints-96754 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T11:04:24Z |
| publishDate | 2021 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | upm.eprints-967542022-12-01T07:30:50Z http://psasir.upm.edu.my/id/eprint/96754/ Discovery of known and novel viruses in wild and cultivated blueberry in Florida through viral metagenomic approaches Saad, Norsazilawati Olmstead, James W. Varsani, Arvind Polston, Jane Elizabeth Jones, Jeffery Folimonova, Svetlana Y. Harmon, Philip F. Southern highbush blueberry (interspecific hybrids of Vaccinium corymbosum L.) is cultivated near wild V. corymbosum as well as closely related species in Florida, USA. The expansion of blueberry cultivation into new areas in Florida and deployment of new cultivars containing viruses can potentially increase the diversity of viruses in wild and cultivated V. corymbosum. In this study, viral diversity in wild and cultivated blueberries (V. corymbosum) is described using a metagenomic approach. RNA viromes from V. corymbosum plants collected from six locations (two cultivated and four wild) in North Central Florida were generated by high throughput sequencing (HTS) and analyzed using a bioinformatic analysis pipeline. De novo assembled contigs obtained from viromes of both commercial and wild sites produced sequences with similarities to plant virus species from a diverse range of families (Amalgaviridae, Caulimoviridae, Endornaviridae, Ophioviridae, Phenuiviridae, and Virgaviridae). In addition, this study has enabled the identification of blueberry latent virus (BlLV) and blueberry mosaic associated ophiovirus (BlMaV) for the first time in Florida, as well as a tentative novel tepovirus (blueberry virus T) (BlVT) in blueberry. To the best of our knowledge, this is the first study that compares viral diversity in wild and cultivated blueberry using a metagenomic approach. Multidisciplinary Digital Publishing Institute 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/96754/1/ABSTRACT.pdf Saad, Norsazilawati and Olmstead, James W. and Varsani, Arvind and Polston, Jane Elizabeth and Jones, Jeffery and Folimonova, Svetlana Y. and Harmon, Philip F. (2021) Discovery of known and novel viruses in wild and cultivated blueberry in Florida through viral metagenomic approaches. Viruses-Basel, 13 (6). pp. 1-16. ISSN 1999-4915 https://www.mdpi.com/1999-4915/13/6/1165 10.3390/v13061165 |
| spellingShingle | Saad, Norsazilawati Olmstead, James W. Varsani, Arvind Polston, Jane Elizabeth Jones, Jeffery Folimonova, Svetlana Y. Harmon, Philip F. Discovery of known and novel viruses in wild and cultivated blueberry in Florida through viral metagenomic approaches |
| title | Discovery of known and novel viruses in wild and cultivated blueberry in Florida through viral metagenomic approaches |
| title_full | Discovery of known and novel viruses in wild and cultivated blueberry in Florida through viral metagenomic approaches |
| title_fullStr | Discovery of known and novel viruses in wild and cultivated blueberry in Florida through viral metagenomic approaches |
| title_full_unstemmed | Discovery of known and novel viruses in wild and cultivated blueberry in Florida through viral metagenomic approaches |
| title_short | Discovery of known and novel viruses in wild and cultivated blueberry in Florida through viral metagenomic approaches |
| title_sort | discovery of known and novel viruses in wild and cultivated blueberry in florida through viral metagenomic approaches |
| url | http://psasir.upm.edu.my/id/eprint/96754/1/ABSTRACT.pdf |
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