Evaluation of Dichlorophenolindophenol (DCIP) test for Haemoglobin E (Hb E) in normal red cell indices individuals
The current cut-off value for thalassaemia screening is based on a mean corpuscular haemoglobin (MCH) level of less than 27 pg. Samples with MCH < 27 pg require additional confirmatory testing, while samples with MCH > 27 pg are considered normal. However, there is a possibility of missing som...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/97154/3/2021120708140602_MJMHS_0524.pdf |
| _version_ | 1825937820724756480 |
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| author | Mohd. Yusoff, Mohd. Fauzi Mohd Tohit, Eusni Rahayu Hashim, Hafizah Seman, Zainina |
| author_facet | Mohd. Yusoff, Mohd. Fauzi Mohd Tohit, Eusni Rahayu Hashim, Hafizah Seman, Zainina |
| author_sort | Mohd. Yusoff, Mohd. Fauzi |
| collection | UPM |
| description | The current cut-off value for thalassaemia screening is based on a mean corpuscular haemoglobin (MCH) level of less than 27 pg. Samples with MCH < 27 pg require additional confirmatory testing, while samples with MCH > 27 pg are considered normal. However, there is a possibility of missing some types of haemoglobinopathy such as haemoglobin (Hb) E trait, by using this cut-off value. This study aimed to evaluate the dichloroindophenol (DCIP) test for Hb E carriers within healthy individuals. Methods: In total, 200 leftover blood samples with normal full blood count (FBC) results and MCH ≥ 27 pg were collected from students who were involved in the thalassaemia
screening program. Blood samples were screened for Hb E by the DCIP test and subjected to haemoglobin analysis using capillary electrophoresis (CE) and gel electrophoresis. Results: Of the 200 leftover blood samples with normal FBC indices, 10 were positive for the DCIP test of which 8 had Hb E trait and 2 had Hb Constant Spring. The DCIP test showed 100% sensitivity and 98.96% specificity with a 100% negative predictive value (NPV) and an 80.0 % positive predictive value (PPV). Conclusion: The DCIP test was an excellent screening tool that, when combined with FBC parameters can produce better results, particularly in areas with a high Hb E incidence. |
| first_indexed | 2024-03-06T11:05:20Z |
| format | Article |
| id | upm.eprints-97154 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T11:05:20Z |
| publishDate | 2021 |
| publisher | Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-971542022-09-14T01:29:32Z http://psasir.upm.edu.my/id/eprint/97154/ Evaluation of Dichlorophenolindophenol (DCIP) test for Haemoglobin E (Hb E) in normal red cell indices individuals Mohd. Yusoff, Mohd. Fauzi Mohd Tohit, Eusni Rahayu Hashim, Hafizah Seman, Zainina The current cut-off value for thalassaemia screening is based on a mean corpuscular haemoglobin (MCH) level of less than 27 pg. Samples with MCH < 27 pg require additional confirmatory testing, while samples with MCH > 27 pg are considered normal. However, there is a possibility of missing some types of haemoglobinopathy such as haemoglobin (Hb) E trait, by using this cut-off value. This study aimed to evaluate the dichloroindophenol (DCIP) test for Hb E carriers within healthy individuals. Methods: In total, 200 leftover blood samples with normal full blood count (FBC) results and MCH ≥ 27 pg were collected from students who were involved in the thalassaemia screening program. Blood samples were screened for Hb E by the DCIP test and subjected to haemoglobin analysis using capillary electrophoresis (CE) and gel electrophoresis. Results: Of the 200 leftover blood samples with normal FBC indices, 10 were positive for the DCIP test of which 8 had Hb E trait and 2 had Hb Constant Spring. The DCIP test showed 100% sensitivity and 98.96% specificity with a 100% negative predictive value (NPV) and an 80.0 % positive predictive value (PPV). Conclusion: The DCIP test was an excellent screening tool that, when combined with FBC parameters can produce better results, particularly in areas with a high Hb E incidence. Universiti Putra Malaysia 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/97154/3/2021120708140602_MJMHS_0524.pdf Mohd. Yusoff, Mohd. Fauzi and Mohd Tohit, Eusni Rahayu and Hashim, Hafizah and Seman, Zainina (2021) Evaluation of Dichlorophenolindophenol (DCIP) test for Haemoglobin E (Hb E) in normal red cell indices individuals. Malaysian Journal of Medicine and Health Sciences, 17 (suppl.10). pp. 10-13. ISSN 2636-9346 https://medic.upm.edu.my/upload/dokumen/2021120708140602_MJMHS_0524.pdf |
| spellingShingle | Mohd. Yusoff, Mohd. Fauzi Mohd Tohit, Eusni Rahayu Hashim, Hafizah Seman, Zainina Evaluation of Dichlorophenolindophenol (DCIP) test for Haemoglobin E (Hb E) in normal red cell indices individuals |
| title | Evaluation of Dichlorophenolindophenol (DCIP) test for Haemoglobin E (Hb E) in normal red cell indices individuals |
| title_full | Evaluation of Dichlorophenolindophenol (DCIP) test for Haemoglobin E (Hb E) in normal red cell indices individuals |
| title_fullStr | Evaluation of Dichlorophenolindophenol (DCIP) test for Haemoglobin E (Hb E) in normal red cell indices individuals |
| title_full_unstemmed | Evaluation of Dichlorophenolindophenol (DCIP) test for Haemoglobin E (Hb E) in normal red cell indices individuals |
| title_short | Evaluation of Dichlorophenolindophenol (DCIP) test for Haemoglobin E (Hb E) in normal red cell indices individuals |
| title_sort | evaluation of dichlorophenolindophenol dcip test for haemoglobin e hb e in normal red cell indices individuals |
| url | http://psasir.upm.edu.my/id/eprint/97154/3/2021120708140602_MJMHS_0524.pdf |
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