Oncolytic activity of velogenic and lentogenic Newcastle Disease Virus (NDV) on Saos-2 cells in hypoxic condition
Introduction: The standard treatment of cancer (surgery, chemotherapy, and radiotherapy) has not improved the survival rate of the patients much over the decades. A new alternative treatment known as oncolytic virotherapy has emerged, utilising selective virus to kill cancer cells. Newcastle diseas...
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| Format: | Article |
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Universiti Putra Malaysia
2019
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| author | Kosni, Nurneqman Nashreq Shafee, Norazizah Omar, Abdul Rahman Suet, Lin Chia |
| author_facet | Kosni, Nurneqman Nashreq Shafee, Norazizah Omar, Abdul Rahman Suet, Lin Chia |
| author_sort | Kosni, Nurneqman Nashreq |
| collection | UPM |
| description | Introduction: The standard treatment of cancer (surgery, chemotherapy, and radiotherapy) has not improved the survival rate of the patients much over the decades. A new alternative treatment
known as oncolytic virotherapy has emerged, utilising selective virus to kill cancer cells. Newcastle disease virus (NDV) is one of the viruses studied for its oncolytic properties. Despite extensive
studies have been done, the relationship between the oncolytic activities of different pathotypes (velogenic and lentogenic) of NDV in Saos-2, either in normoxic or hypoxic conditions, have yet
to be elucidated. Methods: Saos-2 cells grown in normoxic and hypoxic conditions were infected with different pathotypes of NDV. The cells were then analysed with MTT assay as well as flow cytometry and microscopic analyses. Results: It was found that the cell viability reduced to 57% and 75% when velogenic and lentogenic NDV infected the cells, respectively, in both hypoxic and
normoxic conditions. Similar observations were obtained when in flow cytometry and morphological analyses. Conclusion: Velogenic NDV showed higher oncolytic activities compared to that of the lentogenic NDV in both hypoxic and normoxic conditions. These findings warrant further investigation of velogenic NDV to be a potential candidate to be used for the treatment of Saos-2 cell in the future. |
| first_indexed | 2024-03-06T11:10:24Z |
| format | Article |
| id | upm.eprints-99041 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T11:10:24Z |
| publishDate | 2019 |
| publisher | Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-990412023-01-31T03:38:50Z http://psasir.upm.edu.my/id/eprint/99041/ Oncolytic activity of velogenic and lentogenic Newcastle Disease Virus (NDV) on Saos-2 cells in hypoxic condition Kosni, Nurneqman Nashreq Shafee, Norazizah Omar, Abdul Rahman Suet, Lin Chia Introduction: The standard treatment of cancer (surgery, chemotherapy, and radiotherapy) has not improved the survival rate of the patients much over the decades. A new alternative treatment known as oncolytic virotherapy has emerged, utilising selective virus to kill cancer cells. Newcastle disease virus (NDV) is one of the viruses studied for its oncolytic properties. Despite extensive studies have been done, the relationship between the oncolytic activities of different pathotypes (velogenic and lentogenic) of NDV in Saos-2, either in normoxic or hypoxic conditions, have yet to be elucidated. Methods: Saos-2 cells grown in normoxic and hypoxic conditions were infected with different pathotypes of NDV. The cells were then analysed with MTT assay as well as flow cytometry and microscopic analyses. Results: It was found that the cell viability reduced to 57% and 75% when velogenic and lentogenic NDV infected the cells, respectively, in both hypoxic and normoxic conditions. Similar observations were obtained when in flow cytometry and morphological analyses. Conclusion: Velogenic NDV showed higher oncolytic activities compared to that of the lentogenic NDV in both hypoxic and normoxic conditions. These findings warrant further investigation of velogenic NDV to be a potential candidate to be used for the treatment of Saos-2 cell in the future. Universiti Putra Malaysia 2019 Article PeerReviewed Kosni, Nurneqman Nashreq and Shafee, Norazizah and Omar, Abdul Rahman and Suet, Lin Chia (2019) Oncolytic activity of velogenic and lentogenic Newcastle Disease Virus (NDV) on Saos-2 cells in hypoxic condition. Asia-Pacific Journal of Molecular Biology and Biotechnology, 27 (suppl.2). p. 107. ISSN 0128-7451 |
| spellingShingle | Kosni, Nurneqman Nashreq Shafee, Norazizah Omar, Abdul Rahman Suet, Lin Chia Oncolytic activity of velogenic and lentogenic Newcastle Disease Virus (NDV) on Saos-2 cells in hypoxic condition |
| title | Oncolytic activity of velogenic and lentogenic Newcastle Disease Virus (NDV) on Saos-2 cells in hypoxic condition |
| title_full | Oncolytic activity of velogenic and lentogenic Newcastle Disease Virus (NDV) on Saos-2 cells in hypoxic condition |
| title_fullStr | Oncolytic activity of velogenic and lentogenic Newcastle Disease Virus (NDV) on Saos-2 cells in hypoxic condition |
| title_full_unstemmed | Oncolytic activity of velogenic and lentogenic Newcastle Disease Virus (NDV) on Saos-2 cells in hypoxic condition |
| title_short | Oncolytic activity of velogenic and lentogenic Newcastle Disease Virus (NDV) on Saos-2 cells in hypoxic condition |
| title_sort | oncolytic activity of velogenic and lentogenic newcastle disease virus ndv on saos 2 cells in hypoxic condition |
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