Cloning, Expression And Purification Of Toxocara Canis Recombinant Antigens (Rtes-26, Rtes-32, Rtes-120) And Development Of Serodiagnostic Test For Toxocariasis
In this study, recombinant T. canis DNA which encode for rTES-26, rTES-32 and rTES-120 were produced by cloning of open-reading frames (ORF) of the respective genes via reverse-transcriptase-PCR (RT-PCR) using mRNA extracted from a culture of T. canis second stage larvae into PCR2.1 TOPO vector....
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| Format: | Thesis |
| Language: | English |
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2009
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| Online Access: | http://eprints.usm.my/28927/1/Cloning%2C_expression_and_purification_of_Toxocara_canis_Recombinant_Antigens_%28rTES-26%2C_rTES-32%2C_rTES-120%29_and_development_of_serodiagnostic_test_for_toxocariasis.pdf |
| _version_ | 1825832131944775680 |
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| author | Mohamad, Suharni |
| author_facet | Mohamad, Suharni |
| author_sort | Mohamad, Suharni |
| collection | USM |
| description | In this study, recombinant T. canis DNA which encode for rTES-26, rTES-32 and
rTES-120 were produced by cloning of open-reading frames (ORF) of the respective genes
via reverse-transcriptase-PCR (RT-PCR) using mRNA extracted from a culture of T. canis
second stage larvae into PCR2.1 TOPO vector. Dalam kajian ini, DNA rekombinan T. canis yang mengkodkan antigen rekombinan
rTES-26, rTES-32 dan rTES-120 dihasilkan dengan mengklonkan kerangka bacaan terbuka
gen masing-masing melalui kaedah “reverse-transcriptase-PCR” (RT-PCR) menggunakan
mRNA yang diekstrak daripada kultur larva T. canis peringkat kedua ke dalam vektor
PCR2.1 TOPO. |
| first_indexed | 2024-03-06T14:48:16Z |
| format | Thesis |
| id | usm.eprints-28927 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T14:48:16Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | usm.eprints-289272017-05-15T04:43:29Z http://eprints.usm.my/28927/ Cloning, Expression And Purification Of Toxocara Canis Recombinant Antigens (Rtes-26, Rtes-32, Rtes-120) And Development Of Serodiagnostic Test For Toxocariasis Mohamad, Suharni R5-130.5 General works In this study, recombinant T. canis DNA which encode for rTES-26, rTES-32 and rTES-120 were produced by cloning of open-reading frames (ORF) of the respective genes via reverse-transcriptase-PCR (RT-PCR) using mRNA extracted from a culture of T. canis second stage larvae into PCR2.1 TOPO vector. Dalam kajian ini, DNA rekombinan T. canis yang mengkodkan antigen rekombinan rTES-26, rTES-32 dan rTES-120 dihasilkan dengan mengklonkan kerangka bacaan terbuka gen masing-masing melalui kaedah “reverse-transcriptase-PCR” (RT-PCR) menggunakan mRNA yang diekstrak daripada kultur larva T. canis peringkat kedua ke dalam vektor PCR2.1 TOPO. 2009 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/28927/1/Cloning%2C_expression_and_purification_of_Toxocara_canis_Recombinant_Antigens_%28rTES-26%2C_rTES-32%2C_rTES-120%29_and_development_of_serodiagnostic_test_for_toxocariasis.pdf Mohamad, Suharni (2009) Cloning, Expression And Purification Of Toxocara Canis Recombinant Antigens (Rtes-26, Rtes-32, Rtes-120) And Development Of Serodiagnostic Test For Toxocariasis. PhD thesis, Universiti Sains Malaysia. |
| spellingShingle | R5-130.5 General works Mohamad, Suharni Cloning, Expression And Purification Of Toxocara Canis Recombinant Antigens (Rtes-26, Rtes-32, Rtes-120) And Development Of Serodiagnostic Test For Toxocariasis |
| title | Cloning, Expression And Purification Of Toxocara Canis Recombinant Antigens (Rtes-26, Rtes-32, Rtes-120) And Development Of Serodiagnostic Test For Toxocariasis |
| title_full | Cloning, Expression And Purification Of Toxocara Canis Recombinant Antigens (Rtes-26, Rtes-32, Rtes-120) And Development Of Serodiagnostic Test For Toxocariasis |
| title_fullStr | Cloning, Expression And Purification Of Toxocara Canis Recombinant Antigens (Rtes-26, Rtes-32, Rtes-120) And Development Of Serodiagnostic Test For Toxocariasis |
| title_full_unstemmed | Cloning, Expression And Purification Of Toxocara Canis Recombinant Antigens (Rtes-26, Rtes-32, Rtes-120) And Development Of Serodiagnostic Test For Toxocariasis |
| title_short | Cloning, Expression And Purification Of Toxocara Canis Recombinant Antigens (Rtes-26, Rtes-32, Rtes-120) And Development Of Serodiagnostic Test For Toxocariasis |
| title_sort | cloning expression and purification of toxocara canis recombinant antigens rtes 26 rtes 32 rtes 120 and development of serodiagnostic test for toxocariasis |
| topic | R5-130.5 General works |
| url | http://eprints.usm.my/28927/1/Cloning%2C_expression_and_purification_of_Toxocara_canis_Recombinant_Antigens_%28rTES-26%2C_rTES-32%2C_rTES-120%29_and_development_of_serodiagnostic_test_for_toxocariasis.pdf |
| work_keys_str_mv | AT mohamadsuharni cloningexpressionandpurificationoftoxocaracanisrecombinantantigensrtes26rtes32rtes120anddevelopmentofserodiagnostictestfortoxocariasis |