Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization
Feeder layers are commonly used in zebrafish embryonic stem (ES) cell cultures to enable proliferation and prevent differentiation of the ES cells. In this study, a culture method using feeder-free was employed to derive and establish longterm zebrafish ES cell line from zebrafish blastula stage...
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| Format: | Thesis |
| Language: | English |
| Published: |
2014
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| Subjects: | |
| Online Access: | http://eprints.usm.my/29097/1/Establishment_of_a_zebrafish_embryonic_stem_cell_line_using_a_feeder-free_culture_method_and_in_vitro_puripotency_characterization.pdf |
| _version_ | 1825832162890350592 |
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| author | Ho, Sing Yee |
| author_facet | Ho, Sing Yee |
| author_sort | Ho, Sing Yee |
| collection | USM |
| description | Feeder layers are commonly used in zebrafish embryonic stem (ES) cell
cultures to enable proliferation and prevent differentiation of the ES cells. In this
study, a culture method using feeder-free was employed to derive and establish longterm
zebrafish ES cell line from zebrafish blastula stage embryos.
Lapisan sel penyuap telah biasa digunakan dalam kultur sel tunjang
embrionik daripada ikan zebra untuk membolehkan proliferasi dan mencegah
pembezaan sel-sel tersebut. Dalam kajian ini, kaedah kultur tanpa lapisan sel
penyuap telah diguna untuk menghasilkan titisan sel tunjang embrionik jangka masa
panjang daripada embrio peringkat blastula ikan zebra. |
| first_indexed | 2024-03-06T14:48:44Z |
| format | Thesis |
| id | usm.eprints-29097 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T14:48:44Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | usm.eprints-290972019-04-12T05:26:04Z http://eprints.usm.my/29097/ Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization Ho, Sing Yee G Geography (General) SF Animal culture Feeder layers are commonly used in zebrafish embryonic stem (ES) cell cultures to enable proliferation and prevent differentiation of the ES cells. In this study, a culture method using feeder-free was employed to derive and establish longterm zebrafish ES cell line from zebrafish blastula stage embryos. Lapisan sel penyuap telah biasa digunakan dalam kultur sel tunjang embrionik daripada ikan zebra untuk membolehkan proliferasi dan mencegah pembezaan sel-sel tersebut. Dalam kajian ini, kaedah kultur tanpa lapisan sel penyuap telah diguna untuk menghasilkan titisan sel tunjang embrionik jangka masa panjang daripada embrio peringkat blastula ikan zebra. 2014 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/29097/1/Establishment_of_a_zebrafish_embryonic_stem_cell_line_using_a_feeder-free_culture_method_and_in_vitro_puripotency_characterization.pdf Ho, Sing Yee (2014) Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | G Geography (General) SF Animal culture Ho, Sing Yee Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization |
| title | Establishment Of A Zebrafish
Embryonic Stem Cell Line Using
A Feeder-Free Culture Method
And In Vitro Pluripotency
Characterization
|
| title_full | Establishment Of A Zebrafish
Embryonic Stem Cell Line Using
A Feeder-Free Culture Method
And In Vitro Pluripotency
Characterization
|
| title_fullStr | Establishment Of A Zebrafish
Embryonic Stem Cell Line Using
A Feeder-Free Culture Method
And In Vitro Pluripotency
Characterization
|
| title_full_unstemmed | Establishment Of A Zebrafish
Embryonic Stem Cell Line Using
A Feeder-Free Culture Method
And In Vitro Pluripotency
Characterization
|
| title_short | Establishment Of A Zebrafish
Embryonic Stem Cell Line Using
A Feeder-Free Culture Method
And In Vitro Pluripotency
Characterization
|
| title_sort | establishment of a zebrafish embryonic stem cell line using a feeder free culture method and in vitro pluripotency characterization |
| topic | G Geography (General) SF Animal culture |
| url | http://eprints.usm.my/29097/1/Establishment_of_a_zebrafish_embryonic_stem_cell_line_using_a_feeder-free_culture_method_and_in_vitro_puripotency_characterization.pdf |
| work_keys_str_mv | AT hosingyee establishmentofazebrafishembryonicstemcelllineusingafeederfreeculturemethodandinvitropluripotencycharacterization |