Isolation And Establishment Of A New Embryonic-Like Stem Cell Line From Zebrafish

Embryonic stem (ES) cells established from various fish species using feederfree method had been reported in many cases. However, zebrafish ES-like cell lines were currently limited and required the addition of fish embryo extract (FEE) in medium formulation. FEE was not easily available to labor...

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Main Author: Lee, Youn Sing
Format: Thesis
Language:English
Published: 2014
Subjects:
Online Access:http://eprints.usm.my/29108/1/Isolation_and_establishment_of_a_new.pdf
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author Lee, Youn Sing
author_facet Lee, Youn Sing
author_sort Lee, Youn Sing
collection USM
description Embryonic stem (ES) cells established from various fish species using feederfree method had been reported in many cases. However, zebrafish ES-like cell lines were currently limited and required the addition of fish embryo extract (FEE) in medium formulation. FEE was not easily available to laboratories that lack of aquatic housing system. Sel tunjang embrionik (ES) yang dihasilkan daripada pelbagai spesies ikan dengan menggunakan kaedah tanpa sel penyuap telah dilaporkan dalam banyak kes. Walau bagaimanapun, titisan sel tunjang embrionik ikan zebra yang dihasilkan masih terhad dan memerlukan penambahan ekstrak embrio ikan (FEE) dalam formulasi media. FEE tidak mudah didapati oleh makmal-makmal yang kekurangan sistem perumahan akuatik.
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spelling usm.eprints-291082019-04-12T05:26:04Z http://eprints.usm.my/29108/ Isolation And Establishment Of A New Embryonic-Like Stem Cell Line From Zebrafish Lee, Youn Sing QH1 Natural history (General - Including nature conservation, geographical distribution) Embryonic stem (ES) cells established from various fish species using feederfree method had been reported in many cases. However, zebrafish ES-like cell lines were currently limited and required the addition of fish embryo extract (FEE) in medium formulation. FEE was not easily available to laboratories that lack of aquatic housing system. Sel tunjang embrionik (ES) yang dihasilkan daripada pelbagai spesies ikan dengan menggunakan kaedah tanpa sel penyuap telah dilaporkan dalam banyak kes. Walau bagaimanapun, titisan sel tunjang embrionik ikan zebra yang dihasilkan masih terhad dan memerlukan penambahan ekstrak embrio ikan (FEE) dalam formulasi media. FEE tidak mudah didapati oleh makmal-makmal yang kekurangan sistem perumahan akuatik. 2014 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/29108/1/Isolation_and_establishment_of_a_new.pdf Lee, Youn Sing (2014) Isolation And Establishment Of A New Embryonic-Like Stem Cell Line From Zebrafish. Masters thesis, Universiti Sains Malaysia.
spellingShingle QH1 Natural history (General - Including nature conservation, geographical distribution)
Lee, Youn Sing
Isolation And Establishment Of A New Embryonic-Like Stem Cell Line From Zebrafish
title Isolation And Establishment Of A New Embryonic-Like Stem Cell Line From Zebrafish
title_full Isolation And Establishment Of A New Embryonic-Like Stem Cell Line From Zebrafish
title_fullStr Isolation And Establishment Of A New Embryonic-Like Stem Cell Line From Zebrafish
title_full_unstemmed Isolation And Establishment Of A New Embryonic-Like Stem Cell Line From Zebrafish
title_short Isolation And Establishment Of A New Embryonic-Like Stem Cell Line From Zebrafish
title_sort isolation and establishment of a new embryonic like stem cell line from zebrafish
topic QH1 Natural history (General - Including nature conservation, geographical distribution)
url http://eprints.usm.my/29108/1/Isolation_and_establishment_of_a_new.pdf
work_keys_str_mv AT leeyounsing isolationandestablishmentofanewembryoniclikestemcelllinefromzebrafish