| Summary: | Komuniti mikrob bagi dua tanah paya bakau Pulau Pinang (Batu Maung dan Balik Pulau) yang dipengaruhi oleh aktiviti antropogenik telah dikaji dengan menggunakan pendekatan penjujukan metagenomik “shotgun” tanpa-kultur. Dua set
data metagenomik (~250 GB) dihasilkan melalui platfom “Next-generation Sequencing (NGS)” Illumina HiSeq dan disimpan dalam pelayan awam “Metagenomic-Rapid Annotations using Subsystems Technology (MG-RAST)”. Analisis taksonomi mikrob menunjukkan bahawa kedua-dua tanah paya bakau Pulau
Pinang didominasi oleh Bakteria (97 %), Proteobakteria (43 %) dan Deltaproteobakteria (15 %) pada peringkat domain,. filum dan kelas masing-masing. Pada peringkat genus, kebanyakan bakteria anaerobik diperhatikan terdiri daripada
Deltaproteobakteria. Sebahagian besar daripada jujukan adalah milik spesis mikrob (70 %) dan filum (32 %) yang belum dikenalpasti atau belum dikultur.
The microbial communities of two local Penang mangrove soils (Batu Maung
and Balik Pulau) which are under anthropogenic influences were investigated using
culture-independent shotgun metagenome sequencing approach. Two metagenome
data sets (~250 GB) were generated from the Illumina HiSeq next-generation
sequencing (NGS) platform and then deposited in Metagenomic-Rapid Annotations
using Subsystems Technology (MG-RAST) public server. Microbial taxonomic
analysis showed that both Penang mangrove soils were dominated by Bacteria
(97 %), Proteobacteria (43 %) and Deltaproteobacteria (15 %) at the domain,
phylum and class levels, respectively. At the genus level, predominance of anaerobic
bacteria was observed and mostly belonged to Deltaproteobacteria. A large portion
of the reads belonged to unknown or yet uncultured microbial species (70 %) and
microbial phyla (32 %).
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