Heterologous Expression Of Avian Influenza A (H5n1) Neuraminidase In Kluyveromyces Lactis And Escherichia Coli
Pengekspresan neuraminidase (NA) virus H5N1 di dalam sistem pengekspresan mikrob yang ringkas dengan menggunakan Kluyveromyces lactis dan Escherichia coli sebagai perumah telah dikaji. Untuk penghasilan protein NA dalam K. lactis, dua jenis fragmen DNA NA terdiri daripada 1) jujukan penuh (1347 b...
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| Format: | Thesis |
| Language: | English |
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2016
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| Online Access: | http://eprints.usm.my/31644/1/Noor_Zafiah_Taufik.pdf |
| _version_ | 1825832578838429696 |
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| author | Taufik, Noor Zafiah |
| author_facet | Taufik, Noor Zafiah |
| author_sort | Taufik, Noor Zafiah |
| collection | USM |
| description | Pengekspresan neuraminidase (NA) virus H5N1 di dalam sistem
pengekspresan mikrob yang ringkas dengan menggunakan Kluyveromyces lactis dan
Escherichia coli sebagai perumah telah dikaji. Untuk penghasilan protein NA dalam
K. lactis, dua jenis fragmen DNA NA terdiri daripada 1) jujukan penuh (1347 bp)
dan 2) jujukan domain kepala (residu 63-449, 1161 bp) NA daripada
A/Chicken/Malaysia/5858/2004 (H5N1) telah diamplikasi dan diklon ke dalam
vektor pengekspresan pKLAC2 lalu menghasilkan konstruk plasmid yang dinamakan
pKLAC2-NA and pKLAC2-NAHD.
The expression of H5N1 virus neuraminidase (NA) in simple microbial
expression system utilizing Kluyveromyces lactis and Escherichia coli as the host
was studied. For NA protein production in K. lactis, two types of NA DNA fragment
composed of 1) full length sequence (1347 bp) and 2) head domain sequence
(residue 63-449, 1161 bp) from A/Chicken/Malaysia/5858/2004 (H5N1) were
amplified and cloned into pKLAC2 expression vector generating plasmid constructs
named pKLAC2-NA and pKLAC2-NAHD. |
| first_indexed | 2024-03-06T14:54:56Z |
| format | Thesis |
| id | usm.eprints-31644 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T14:54:56Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | usm.eprints-316442019-04-12T05:25:14Z http://eprints.usm.my/31644/ Heterologous Expression Of Avian Influenza A (H5n1) Neuraminidase In Kluyveromyces Lactis And Escherichia Coli Taufik, Noor Zafiah QH1 Natural history (General - Including nature conservation, geographical distribution) Pengekspresan neuraminidase (NA) virus H5N1 di dalam sistem pengekspresan mikrob yang ringkas dengan menggunakan Kluyveromyces lactis dan Escherichia coli sebagai perumah telah dikaji. Untuk penghasilan protein NA dalam K. lactis, dua jenis fragmen DNA NA terdiri daripada 1) jujukan penuh (1347 bp) dan 2) jujukan domain kepala (residu 63-449, 1161 bp) NA daripada A/Chicken/Malaysia/5858/2004 (H5N1) telah diamplikasi dan diklon ke dalam vektor pengekspresan pKLAC2 lalu menghasilkan konstruk plasmid yang dinamakan pKLAC2-NA and pKLAC2-NAHD. The expression of H5N1 virus neuraminidase (NA) in simple microbial expression system utilizing Kluyveromyces lactis and Escherichia coli as the host was studied. For NA protein production in K. lactis, two types of NA DNA fragment composed of 1) full length sequence (1347 bp) and 2) head domain sequence (residue 63-449, 1161 bp) from A/Chicken/Malaysia/5858/2004 (H5N1) were amplified and cloned into pKLAC2 expression vector generating plasmid constructs named pKLAC2-NA and pKLAC2-NAHD. 2016-09 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/31644/1/Noor_Zafiah_Taufik.pdf Taufik, Noor Zafiah (2016) Heterologous Expression Of Avian Influenza A (H5n1) Neuraminidase In Kluyveromyces Lactis And Escherichia Coli. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | QH1 Natural history (General - Including nature conservation, geographical distribution) Taufik, Noor Zafiah Heterologous Expression Of Avian Influenza A (H5n1) Neuraminidase In Kluyveromyces Lactis And Escherichia Coli |
| title | Heterologous Expression Of Avian
Influenza A (H5n1) Neuraminidase In
Kluyveromyces Lactis And Escherichia Coli |
| title_full | Heterologous Expression Of Avian
Influenza A (H5n1) Neuraminidase In
Kluyveromyces Lactis And Escherichia Coli |
| title_fullStr | Heterologous Expression Of Avian
Influenza A (H5n1) Neuraminidase In
Kluyveromyces Lactis And Escherichia Coli |
| title_full_unstemmed | Heterologous Expression Of Avian
Influenza A (H5n1) Neuraminidase In
Kluyveromyces Lactis And Escherichia Coli |
| title_short | Heterologous Expression Of Avian
Influenza A (H5n1) Neuraminidase In
Kluyveromyces Lactis And Escherichia Coli |
| title_sort | heterologous expression of avian influenza a h5n1 neuraminidase in kluyveromyces lactis and escherichia coli |
| topic | QH1 Natural history (General - Including nature conservation, geographical distribution) |
| url | http://eprints.usm.my/31644/1/Noor_Zafiah_Taufik.pdf |
| work_keys_str_mv | AT taufiknoorzafiah heterologousexpressionofavianinfluenzaah5n1neuraminidaseinkluyveromyceslactisandescherichiacoli |