Expression, Purification And Crystallization Trials Of Small Rubber Particle Protein (Srpp) From Hevea Brasiliensis
Getah asli daripada Hevea brasiliensis yang diperbuat daripada cis-1,4-poliisopren, merupakan komoditi perusahaan yang penuh dengan ciri-ciri keunikan. Biosintesis getah asli daripada H. brasiliensis berlaku pada permukaan partikel getah yang dimangkinkan oleh ‘small rubber particle protein’ (SRPP)...
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| Format: | Thesis |
| Language: | English |
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2016
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| Online Access: | http://eprints.usm.my/31810/1/SARANPAL_SINGH_AL_SATINDER_SINGH_24%28NN%29.pdf |
| _version_ | 1797008711937425408 |
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| author | Satinder Singh, Saranpal Singh |
| author_facet | Satinder Singh, Saranpal Singh |
| author_sort | Satinder Singh, Saranpal Singh |
| collection | USM |
| description | Getah asli daripada Hevea brasiliensis yang diperbuat daripada cis-1,4-poliisopren, merupakan komoditi perusahaan yang penuh dengan ciri-ciri keunikan. Biosintesis getah asli daripada H. brasiliensis berlaku pada permukaan partikel getah yang dimangkinkan oleh ‘small rubber particle protein’ (SRPP) dan ‘rubber elongation factor’ (REF). Protein SRPP ini dipercayai menggabungkan isopentenil difosfat (IPP) pada permukaan partikel getah. Tambahan pula, protein SRPP juga merupakan salah satu alergen getah utama dalam H. brasiliensis. Walaupun terdapat banyak bukti yang menunjukkan penglibatan protein ini dalam biosintesis getah dan alergen, namun, masih terdapat kekurangan penyelidikan yang mengaitkan mekanisme SRPP dalam sintesis getah dan mekanisme alahan.
Natural rubber (NR) of Hevea brasiliensis predominantly made up of cis-1,4-polyisoprene, is an essential industrial commodity with unique characteristics. The biosynthesis of NR from H. brasiliensis is catalyzed on the surface of rubber particles by a set of integrated proteins, namely the small rubber particle protein (SRPP), and rubber elongation factor (REF). The SRPP protein has been speculated to incorporate isopentenyl diphosphate (IPP) monomers in rubber particles. Furthermore, the SRPP protein has also been implicated as a major latex allergen.
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| first_indexed | 2024-03-06T14:55:17Z |
| format | Thesis |
| id | usm.eprints-31810 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T14:55:17Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | usm.eprints-318102019-04-12T05:25:26Z http://eprints.usm.my/31810/ Expression, Purification And Crystallization Trials Of Small Rubber Particle Protein (Srpp) From Hevea Brasiliensis Satinder Singh, Saranpal Singh QH1 Natural history (General - Including nature conservation, geographical distribution) Getah asli daripada Hevea brasiliensis yang diperbuat daripada cis-1,4-poliisopren, merupakan komoditi perusahaan yang penuh dengan ciri-ciri keunikan. Biosintesis getah asli daripada H. brasiliensis berlaku pada permukaan partikel getah yang dimangkinkan oleh ‘small rubber particle protein’ (SRPP) dan ‘rubber elongation factor’ (REF). Protein SRPP ini dipercayai menggabungkan isopentenil difosfat (IPP) pada permukaan partikel getah. Tambahan pula, protein SRPP juga merupakan salah satu alergen getah utama dalam H. brasiliensis. Walaupun terdapat banyak bukti yang menunjukkan penglibatan protein ini dalam biosintesis getah dan alergen, namun, masih terdapat kekurangan penyelidikan yang mengaitkan mekanisme SRPP dalam sintesis getah dan mekanisme alahan. Natural rubber (NR) of Hevea brasiliensis predominantly made up of cis-1,4-polyisoprene, is an essential industrial commodity with unique characteristics. The biosynthesis of NR from H. brasiliensis is catalyzed on the surface of rubber particles by a set of integrated proteins, namely the small rubber particle protein (SRPP), and rubber elongation factor (REF). The SRPP protein has been speculated to incorporate isopentenyl diphosphate (IPP) monomers in rubber particles. Furthermore, the SRPP protein has also been implicated as a major latex allergen. 2016-02 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/31810/1/SARANPAL_SINGH_AL_SATINDER_SINGH_24%28NN%29.pdf Satinder Singh, Saranpal Singh (2016) Expression, Purification And Crystallization Trials Of Small Rubber Particle Protein (Srpp) From Hevea Brasiliensis. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | QH1 Natural history (General - Including nature conservation, geographical distribution) Satinder Singh, Saranpal Singh Expression, Purification And Crystallization Trials Of Small Rubber Particle Protein (Srpp) From Hevea Brasiliensis |
| title | Expression, Purification And Crystallization Trials Of Small Rubber Particle Protein (Srpp) From Hevea Brasiliensis |
| title_full | Expression, Purification And Crystallization Trials Of Small Rubber Particle Protein (Srpp) From Hevea Brasiliensis |
| title_fullStr | Expression, Purification And Crystallization Trials Of Small Rubber Particle Protein (Srpp) From Hevea Brasiliensis |
| title_full_unstemmed | Expression, Purification And Crystallization Trials Of Small Rubber Particle Protein (Srpp) From Hevea Brasiliensis |
| title_short | Expression, Purification And Crystallization Trials Of Small Rubber Particle Protein (Srpp) From Hevea Brasiliensis |
| title_sort | expression purification and crystallization trials of small rubber particle protein srpp from hevea brasiliensis |
| topic | QH1 Natural history (General - Including nature conservation, geographical distribution) |
| url | http://eprints.usm.my/31810/1/SARANPAL_SINGH_AL_SATINDER_SINGH_24%28NN%29.pdf |
| work_keys_str_mv | AT satindersinghsaranpalsingh expressionpurificationandcrystallizationtrialsofsmallrubberparticleproteinsrppfromheveabrasiliensis |