Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies
Diagnostik ‘point-of-care’ (POC) untuk kawasan terpencil telah menjadi cabaran besar untuk memerangi penyakit berjangkit di seluruh dunia. Pembangunan kaedah untuk membantu proses pembangunan diagnostik POC baru adalah penting untuk memperbaiki senario penjagaan kesihatan di seluruh dunia. Format ut...
| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2016
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| Online Access: | http://eprints.usm.my/31823/1/NUR_FAEZEE_BINTI_ISMAIL_24%28NN%29.pdf |
| _version_ | 1825832604181463040 |
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| author | Ismail, Nur Faezee |
| author_facet | Ismail, Nur Faezee |
| author_sort | Ismail, Nur Faezee |
| collection | USM |
| description | Diagnostik ‘point-of-care’ (POC) untuk kawasan terpencil telah menjadi cabaran besar untuk memerangi penyakit berjangkit di seluruh dunia. Pembangunan kaedah untuk membantu proses pembangunan diagnostik POC baru adalah penting untuk memperbaiki senario penjagaan kesihatan di seluruh dunia. Format utama POC asai adalah pengesanan interaksi antibodi- antigen . Untuk mencapai matlamat ini, asai yang mampu melaporkan interaksi antara antibodi dan antigen adalah diperlukan. Kebiasaannya. asai dibangunkan dengan merangkaikan antibodi-antibodi kepada satu pelapor yang spesifik seperti enzim atau protein. Projek ini menerangkan penggunaan transpeptidasi berantarakan sortase sebagai satu cara untuk merangkaikan antibodi rekombinan untuk sepasang protein pelapor. Enzim Sortase adalah unik dengan mengenali motif yang mempunyai urutan yang khusus untuk membenarkan tidak balas rangkaian protein-protein secara setempat.
Point-of-care (POC) diagnostics for resource deprived regions remains a major challenge to combat infectious diseases worldwide. Development of new methods to aid the development process of new POC diagnostics is essential to improve the healthcare scenario worldwide. The major format of POC assay is the detection of antibody-antigen interaction. To achieve this, an assay is required to be capable of reporting the interaction between the antibody and antigen. In most common assays, these detection are achieved by conjugating antibodies to specific reporter molecules such as enzymes or proteins. The work describes the application of sortase mediated transpeptidation as a means to conjugate recombinant antibodies to a pair of reporter proteins. |
| first_indexed | 2024-03-06T14:55:19Z |
| format | Thesis |
| id | usm.eprints-31823 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T14:55:19Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | usm.eprints-318232019-04-12T05:25:22Z http://eprints.usm.my/31823/ Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies Ismail, Nur Faezee R735-854 Medical education. Medical schools. Research Diagnostik ‘point-of-care’ (POC) untuk kawasan terpencil telah menjadi cabaran besar untuk memerangi penyakit berjangkit di seluruh dunia. Pembangunan kaedah untuk membantu proses pembangunan diagnostik POC baru adalah penting untuk memperbaiki senario penjagaan kesihatan di seluruh dunia. Format utama POC asai adalah pengesanan interaksi antibodi- antigen . Untuk mencapai matlamat ini, asai yang mampu melaporkan interaksi antara antibodi dan antigen adalah diperlukan. Kebiasaannya. asai dibangunkan dengan merangkaikan antibodi-antibodi kepada satu pelapor yang spesifik seperti enzim atau protein. Projek ini menerangkan penggunaan transpeptidasi berantarakan sortase sebagai satu cara untuk merangkaikan antibodi rekombinan untuk sepasang protein pelapor. Enzim Sortase adalah unik dengan mengenali motif yang mempunyai urutan yang khusus untuk membenarkan tidak balas rangkaian protein-protein secara setempat. Point-of-care (POC) diagnostics for resource deprived regions remains a major challenge to combat infectious diseases worldwide. Development of new methods to aid the development process of new POC diagnostics is essential to improve the healthcare scenario worldwide. The major format of POC assay is the detection of antibody-antigen interaction. To achieve this, an assay is required to be capable of reporting the interaction between the antibody and antigen. In most common assays, these detection are achieved by conjugating antibodies to specific reporter molecules such as enzymes or proteins. The work describes the application of sortase mediated transpeptidation as a means to conjugate recombinant antibodies to a pair of reporter proteins. 2016-03 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/31823/1/NUR_FAEZEE_BINTI_ISMAIL_24%28NN%29.pdf Ismail, Nur Faezee (2016) Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | R735-854 Medical education. Medical schools. Research Ismail, Nur Faezee Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies |
| title | Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies |
| title_full | Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies |
| title_fullStr | Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies |
| title_full_unstemmed | Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies |
| title_short | Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies |
| title_sort | sortase mediated transpeptidation the application of sortase a as a tool for site specific modification of recombinant antibodies |
| topic | R735-854 Medical education. Medical schools. Research |
| url | http://eprints.usm.my/31823/1/NUR_FAEZEE_BINTI_ISMAIL_24%28NN%29.pdf |
| work_keys_str_mv | AT ismailnurfaezee sortasemediatedtranspeptidationtheapplicationofsortaseaasatoolforsitespecificmodificationofrecombinantantibodies |