Effects of Nicotine Administration and Nicotine Cessation on Bone Histomorphometry and Bone Biomarkers in Sprague–Dawley Male Rats
Nicotine is a major alkaloid of tobacco, which can increase free radical formation, leading to osteoporosis. The effects of nicotine administration and cessation on bone histomorphometry and biomarkers were studied in 28 Sprague–Dawley male rats. Rats aged 3 months and weighing 250–300 g were d...
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| Format: | Article |
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2011
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| author | Hapidin, Hermizi Othman, Faizah Soelaiman, Ima-Nirwana Shuid, Ahmad Nazrun Mohamed, Norazlina |
| author_facet | Hapidin, Hermizi Othman, Faizah Soelaiman, Ima-Nirwana Shuid, Ahmad Nazrun Mohamed, Norazlina |
| author_sort | Hapidin, Hermizi |
| collection | USM |
| description | Nicotine is a major alkaloid of tobacco, which
can increase free radical formation, leading to osteoporosis.
The effects of nicotine administration and cessation on
bone histomorphometry and biomarkers were studied in 28
Sprague–Dawley male rats. Rats aged 3 months and
weighing 250–300 g were divided into four groups: control
(C, normal saline for 4 months), nicotine for 2 months
(N2), nicotine for 4 months (N4), and nicotine cessation
(NC). The NC group was given nicotine for the first
2 months and then allowed to recover for the following
2 months without nicotine. Histomorphometric analysis
was done using an image analyzer. ELISA kits were used
to measure serum osteocalcin (bone formation marker) and
pyridinoline (PYD, bone resorption marker) levels at
month 0, month 2, and month 4. All test groups showed a
significant decrease in BV/TV, Ob.S/BS, dLS/BS, MAR,
BFR/BS, and osteocalcin levels and an increase in sLS/BS
and PYD levels compared to group C. No significant differences
were observed in all parameters measured among
the test groups, except for MAR and BFR/BS. In conclusion,
nicotine administration at a dose of 7 mg/kg for 2 and
4 months has detrimental effects on bone metabolism.
Nicotine administration at 7 mg/kg for 2 months is sufficient
to produce significant effects on bone histomorphometric
parameters and biomarkers. In addition, prolonging
the treatment for another 2 months did not show any significant
differences. Cessation of nicotine for 2 months did
not reverse the effects. |
| first_indexed | 2024-03-06T15:06:20Z |
| format | Article |
| id | usm.eprints-35760 |
| institution | Universiti Sains Malaysia |
| last_indexed | 2024-03-06T15:06:20Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | usm.eprints-357602017-07-21T00:59:10Z http://eprints.usm.my/35760/ Effects of Nicotine Administration and Nicotine Cessation on Bone Histomorphometry and Bone Biomarkers in Sprague–Dawley Male Rats Hapidin, Hermizi Othman, Faizah Soelaiman, Ima-Nirwana Shuid, Ahmad Nazrun Mohamed, Norazlina RA0421 Public health. Hygiene. Preventive Medicine Nicotine is a major alkaloid of tobacco, which can increase free radical formation, leading to osteoporosis. The effects of nicotine administration and cessation on bone histomorphometry and biomarkers were studied in 28 Sprague–Dawley male rats. Rats aged 3 months and weighing 250–300 g were divided into four groups: control (C, normal saline for 4 months), nicotine for 2 months (N2), nicotine for 4 months (N4), and nicotine cessation (NC). The NC group was given nicotine for the first 2 months and then allowed to recover for the following 2 months without nicotine. Histomorphometric analysis was done using an image analyzer. ELISA kits were used to measure serum osteocalcin (bone formation marker) and pyridinoline (PYD, bone resorption marker) levels at month 0, month 2, and month 4. All test groups showed a significant decrease in BV/TV, Ob.S/BS, dLS/BS, MAR, BFR/BS, and osteocalcin levels and an increase in sLS/BS and PYD levels compared to group C. No significant differences were observed in all parameters measured among the test groups, except for MAR and BFR/BS. In conclusion, nicotine administration at a dose of 7 mg/kg for 2 and 4 months has detrimental effects on bone metabolism. Nicotine administration at 7 mg/kg for 2 months is sufficient to produce significant effects on bone histomorphometric parameters and biomarkers. In addition, prolonging the treatment for another 2 months did not show any significant differences. Cessation of nicotine for 2 months did not reverse the effects. 2011 Article PeerReviewed Hapidin, Hermizi and Othman, Faizah and Soelaiman, Ima-Nirwana and Shuid, Ahmad Nazrun and Mohamed, Norazlina (2011) Effects of Nicotine Administration and Nicotine Cessation on Bone Histomorphometry and Bone Biomarkers in Sprague–Dawley Male Rats. Calcif Tissue Int, 88. pp. 41-47. https://doi.org/10.1007/s00223-010-9426-4 |
| spellingShingle | RA0421 Public health. Hygiene. Preventive Medicine Hapidin, Hermizi Othman, Faizah Soelaiman, Ima-Nirwana Shuid, Ahmad Nazrun Mohamed, Norazlina Effects of Nicotine Administration and Nicotine Cessation on Bone Histomorphometry and Bone Biomarkers in Sprague–Dawley Male Rats |
| title | Effects of Nicotine Administration and Nicotine Cessation on Bone
Histomorphometry and Bone Biomarkers in Sprague–Dawley
Male Rats |
| title_full | Effects of Nicotine Administration and Nicotine Cessation on Bone
Histomorphometry and Bone Biomarkers in Sprague–Dawley
Male Rats |
| title_fullStr | Effects of Nicotine Administration and Nicotine Cessation on Bone
Histomorphometry and Bone Biomarkers in Sprague–Dawley
Male Rats |
| title_full_unstemmed | Effects of Nicotine Administration and Nicotine Cessation on Bone
Histomorphometry and Bone Biomarkers in Sprague–Dawley
Male Rats |
| title_short | Effects of Nicotine Administration and Nicotine Cessation on Bone
Histomorphometry and Bone Biomarkers in Sprague–Dawley
Male Rats |
| title_sort | effects of nicotine administration and nicotine cessation on bone histomorphometry and bone biomarkers in sprague dawley male rats |
| topic | RA0421 Public health. Hygiene. Preventive Medicine |
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