In Vitro Growth of Human Keratinocytes and Oral Cancer Cells into Microtissues: An Aerosol-Based Microencapsulation Technique
Cells encapsulation is a micro-technology widely applied in cell and tissue research, tissue transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line (ORL...
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| Format: | Article |
| Language: | English |
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MDPI
2017
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| Online Access: | http://eprints.usm.my/36986/1/%28In_Vitro_Growth_of_Human_%29__bioengineering-04-00043.pdf |
| _version_ | 1825833574294618112 |
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| author | Wai, Yean Leong Chin, Fhong Soon Soon, Chuan Wong Kian, Sek Tee Sok, Ching Cheong Siew, Hua Gan Youseffi, Mansour |
| author_facet | Wai, Yean Leong Chin, Fhong Soon Soon, Chuan Wong Kian, Sek Tee Sok, Ching Cheong Siew, Hua Gan Youseffi, Mansour |
| author_sort | Wai, Yean Leong |
| collection | USM |
| description | Cells encapsulation is a micro-technology widely applied in cell and tissue research, tissue
transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model
for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line
(ORL-48) based on a simple aerosol microencapsulation technique. At an extrusion rate of 20 �L/min
and air flow rate of 0.3 L/min programmed in the aerosol system, HaCaT and ORL-48 cells in alginate
microcapsules were encapsulated in microcapsules with a diameter ranging from 200 to 300 �m.
Both cell lines were successfully grown into microtissues in the microcapsules of alginate within
16 days of culture. The microtissues were characterized by using a live/dead cell viability assay,
field emission-scanning electron microscopy (FE-SEM), fluorescence staining, and cell re-plating
experiments. The microtissues of both cell types were viable after being extracted from the alginate
membrane using alginate lyase. However, the microtissues of HaCaT and ORL-48 demonstrated
differences in both nucleus size and morphology. The microtissues with re-associated cells in
spheroids are potentially useful as a cell model for pharmacological studies. |
| first_indexed | 2024-03-06T15:09:52Z |
| format | Article |
| id | usm.eprints-36986 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:09:52Z |
| publishDate | 2017 |
| publisher | MDPI |
| record_format | dspace |
| spelling | usm.eprints-369862017-10-10T01:17:03Z http://eprints.usm.my/36986/ In Vitro Growth of Human Keratinocytes and Oral Cancer Cells into Microtissues: An Aerosol-Based Microencapsulation Technique Wai, Yean Leong Chin, Fhong Soon Soon, Chuan Wong Kian, Sek Tee Sok, Ching Cheong Siew, Hua Gan Youseffi, Mansour R5-130.5 General works Cells encapsulation is a micro-technology widely applied in cell and tissue research, tissue transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line (ORL-48) based on a simple aerosol microencapsulation technique. At an extrusion rate of 20 �L/min and air flow rate of 0.3 L/min programmed in the aerosol system, HaCaT and ORL-48 cells in alginate microcapsules were encapsulated in microcapsules with a diameter ranging from 200 to 300 �m. Both cell lines were successfully grown into microtissues in the microcapsules of alginate within 16 days of culture. The microtissues were characterized by using a live/dead cell viability assay, field emission-scanning electron microscopy (FE-SEM), fluorescence staining, and cell re-plating experiments. The microtissues of both cell types were viable after being extracted from the alginate membrane using alginate lyase. However, the microtissues of HaCaT and ORL-48 demonstrated differences in both nucleus size and morphology. The microtissues with re-associated cells in spheroids are potentially useful as a cell model for pharmacological studies. MDPI 2017 Article PeerReviewed application/pdf en http://eprints.usm.my/36986/1/%28In_Vitro_Growth_of_Human_%29__bioengineering-04-00043.pdf Wai, Yean Leong and Chin, Fhong Soon and Soon, Chuan Wong and Kian, Sek Tee and Sok, Ching Cheong and Siew, Hua Gan and Youseffi, Mansour (2017) In Vitro Growth of Human Keratinocytes and Oral Cancer Cells into Microtissues: An Aerosol-Based Microencapsulation Technique. Bioengineering, 4 (43). pp. 1-14. ISSN 2306-5354 http://www.mdpi.com/2306-5354/4/2/43 |
| spellingShingle | R5-130.5 General works Wai, Yean Leong Chin, Fhong Soon Soon, Chuan Wong Kian, Sek Tee Sok, Ching Cheong Siew, Hua Gan Youseffi, Mansour In Vitro Growth of Human Keratinocytes and Oral Cancer Cells into Microtissues: An Aerosol-Based Microencapsulation Technique |
| title | In Vitro Growth of Human Keratinocytes and Oral
Cancer Cells into Microtissues: An Aerosol-Based
Microencapsulation Technique |
| title_full | In Vitro Growth of Human Keratinocytes and Oral
Cancer Cells into Microtissues: An Aerosol-Based
Microencapsulation Technique |
| title_fullStr | In Vitro Growth of Human Keratinocytes and Oral
Cancer Cells into Microtissues: An Aerosol-Based
Microencapsulation Technique |
| title_full_unstemmed | In Vitro Growth of Human Keratinocytes and Oral
Cancer Cells into Microtissues: An Aerosol-Based
Microencapsulation Technique |
| title_short | In Vitro Growth of Human Keratinocytes and Oral
Cancer Cells into Microtissues: An Aerosol-Based
Microencapsulation Technique |
| title_sort | in vitro growth of human keratinocytes and oral cancer cells into microtissues an aerosol based microencapsulation technique |
| topic | R5-130.5 General works |
| url | http://eprints.usm.my/36986/1/%28In_Vitro_Growth_of_Human_%29__bioengineering-04-00043.pdf |
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