Phyllanthus Niruri Standardized Extract Alleviates the Progression of Non-Alcoholic Fatty Liver Disease and Decreases Atherosclerotic Risk in Sprague–Dawley Rats
Non-alcoholic fatty liver disease (NAFLD) is one of the major global health issues, strongly correlated with insulin resistance, obesity and oxidative stress. The current study aimed to evaluate anti-NAFLD effects of three different extracts of Phyllanthus niruri (P. niruri). NAFLD was induced in...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI
2017
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| Subjects: | |
| Online Access: | http://eprints.usm.my/37136/1/%28Phyllanthus_Niruri%29_nutrients-09-00766.pdf |
| _version_ | 1797009769007939584 |
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| author | Al Zarzour, Raghdaa Hamdan Ahmad, Mariam Asmawi, Mohd. Zaini Kaur, Gurjeet Ahmed Saeed, Mohammed Ali Al-Mansoub, Majed Ahmed Mohammed Saghir, Sultan Ayesh Usman, Nasiba Salisu Al-Dulaimi, Dhamraa W. Mun, Fei Yam |
| author_facet | Al Zarzour, Raghdaa Hamdan Ahmad, Mariam Asmawi, Mohd. Zaini Kaur, Gurjeet Ahmed Saeed, Mohammed Ali Al-Mansoub, Majed Ahmed Mohammed Saghir, Sultan Ayesh Usman, Nasiba Salisu Al-Dulaimi, Dhamraa W. Mun, Fei Yam |
| author_sort | Al Zarzour, Raghdaa Hamdan |
| collection | USM |
| description | Non-alcoholic fatty liver disease (NAFLD) is one of the major global health issues, strongly
correlated with insulin resistance, obesity and oxidative stress. The current study aimed to evaluate
anti-NAFLD effects of three different extracts of Phyllanthus niruri (P. niruri). NAFLD was induced
in male Sprague–Dawley rats using a special high-fat diet (HFD). A 50% methanolic extract (50%
ME) exhibited the highest inhibitory effect against NAFLD progression. It significantly reduced
hepatomegaly (16%) and visceral fat weight (22%), decreased NAFLD score, prevented fibrosis,
and reduced serum total cholesterol (TC) (48%), low-density lipoprotein (LDL) (65%), free fatty
acids (FFAs) (25%), alanine aminotransferase (ALT) (45%), alkaline phosphatase (ALP) (38%), insulin
concentration (67%), homeostatic model assessment of insulin resistance (HOMA-IR) (73%), serum
atherogenic ratios TC/high-density lipoprotein (HDL) (29%), LDL/HDL (66%) and (TC–HDL)/HDL
(64%), hepatic content of cholesterol (43%), triglyceride (29%) and malondialdehyde (MDA) (40%)
compared to a non-treated HFD group. In vitro, 50% ME of P. niruri inhibited �-glucosidase,
pancreatic lipase enzymes and cholesterol micellization. It also had higher total phenolic and
total flavonoid contents compared to other extracts. Ellagic acid and phyllanthin were identified as
major compounds. These results suggest that P. niruri could be further developed as a novel natural
hepatoprotective agent against NAFLD and atherosclerosis. |
| first_indexed | 2024-03-06T15:10:17Z |
| format | Article |
| id | usm.eprints-37136 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:10:17Z |
| publishDate | 2017 |
| publisher | MDPI |
| record_format | dspace |
| spelling | usm.eprints-371362017-10-16T04:13:09Z http://eprints.usm.my/37136/ Phyllanthus Niruri Standardized Extract Alleviates the Progression of Non-Alcoholic Fatty Liver Disease and Decreases Atherosclerotic Risk in Sprague–Dawley Rats Al Zarzour, Raghdaa Hamdan Ahmad, Mariam Asmawi, Mohd. Zaini Kaur, Gurjeet Ahmed Saeed, Mohammed Ali Al-Mansoub, Majed Ahmed Mohammed Saghir, Sultan Ayesh Usman, Nasiba Salisu Al-Dulaimi, Dhamraa W. Mun, Fei Yam RS1-441 Pharmacy and materia medica Non-alcoholic fatty liver disease (NAFLD) is one of the major global health issues, strongly correlated with insulin resistance, obesity and oxidative stress. The current study aimed to evaluate anti-NAFLD effects of three different extracts of Phyllanthus niruri (P. niruri). NAFLD was induced in male Sprague–Dawley rats using a special high-fat diet (HFD). A 50% methanolic extract (50% ME) exhibited the highest inhibitory effect against NAFLD progression. It significantly reduced hepatomegaly (16%) and visceral fat weight (22%), decreased NAFLD score, prevented fibrosis, and reduced serum total cholesterol (TC) (48%), low-density lipoprotein (LDL) (65%), free fatty acids (FFAs) (25%), alanine aminotransferase (ALT) (45%), alkaline phosphatase (ALP) (38%), insulin concentration (67%), homeostatic model assessment of insulin resistance (HOMA-IR) (73%), serum atherogenic ratios TC/high-density lipoprotein (HDL) (29%), LDL/HDL (66%) and (TC–HDL)/HDL (64%), hepatic content of cholesterol (43%), triglyceride (29%) and malondialdehyde (MDA) (40%) compared to a non-treated HFD group. In vitro, 50% ME of P. niruri inhibited �-glucosidase, pancreatic lipase enzymes and cholesterol micellization. It also had higher total phenolic and total flavonoid contents compared to other extracts. Ellagic acid and phyllanthin were identified as major compounds. These results suggest that P. niruri could be further developed as a novel natural hepatoprotective agent against NAFLD and atherosclerosis. MDPI 2017 Article PeerReviewed application/pdf en http://eprints.usm.my/37136/1/%28Phyllanthus_Niruri%29_nutrients-09-00766.pdf Al Zarzour, Raghdaa Hamdan and Ahmad, Mariam and Asmawi, Mohd. Zaini and Kaur, Gurjeet and Ahmed Saeed, Mohammed Ali and Al-Mansoub, Majed Ahmed and Mohammed Saghir, Sultan Ayesh and Usman, Nasiba Salisu and Al-Dulaimi, Dhamraa W. and Mun, Fei Yam (2017) Phyllanthus Niruri Standardized Extract Alleviates the Progression of Non-Alcoholic Fatty Liver Disease and Decreases Atherosclerotic Risk in Sprague–Dawley Rats. Nutrients, 9 (766). pp. 1-19. ISSN 2072-6643 http://www.mdpi.com/2072-6643/9/7/766 |
| spellingShingle | RS1-441 Pharmacy and materia medica Al Zarzour, Raghdaa Hamdan Ahmad, Mariam Asmawi, Mohd. Zaini Kaur, Gurjeet Ahmed Saeed, Mohammed Ali Al-Mansoub, Majed Ahmed Mohammed Saghir, Sultan Ayesh Usman, Nasiba Salisu Al-Dulaimi, Dhamraa W. Mun, Fei Yam Phyllanthus Niruri Standardized Extract Alleviates the Progression of Non-Alcoholic Fatty Liver Disease and Decreases Atherosclerotic Risk in Sprague–Dawley Rats |
| title | Phyllanthus Niruri Standardized Extract Alleviates
the Progression of Non-Alcoholic Fatty Liver Disease
and Decreases Atherosclerotic Risk in
Sprague–Dawley Rats |
| title_full | Phyllanthus Niruri Standardized Extract Alleviates
the Progression of Non-Alcoholic Fatty Liver Disease
and Decreases Atherosclerotic Risk in
Sprague–Dawley Rats |
| title_fullStr | Phyllanthus Niruri Standardized Extract Alleviates
the Progression of Non-Alcoholic Fatty Liver Disease
and Decreases Atherosclerotic Risk in
Sprague–Dawley Rats |
| title_full_unstemmed | Phyllanthus Niruri Standardized Extract Alleviates
the Progression of Non-Alcoholic Fatty Liver Disease
and Decreases Atherosclerotic Risk in
Sprague–Dawley Rats |
| title_short | Phyllanthus Niruri Standardized Extract Alleviates
the Progression of Non-Alcoholic Fatty Liver Disease
and Decreases Atherosclerotic Risk in
Sprague–Dawley Rats |
| title_sort | phyllanthus niruri standardized extract alleviates the progression of non alcoholic fatty liver disease and decreases atherosclerotic risk in sprague dawley rats |
| topic | RS1-441 Pharmacy and materia medica |
| url | http://eprints.usm.my/37136/1/%28Phyllanthus_Niruri%29_nutrients-09-00766.pdf |
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