Recovery and subsequent characterization of polyhydroxybutyrate from Rhodococcus equi cells grown on crude palm kernel oil
The gram-positive bacterium Rhodococcus equi was isolated from fertile soil, and mineral salt media (MM) and trace elementswere used to provide the necessary elements for its growth and PHB production in addition to using crude palm kernel oil (CPKO) 1%as the carbon source. Gas chromatography (GC) d...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taibah University, Madinah, Kingdom of Saudi Arabia
2016
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/37183/1/%28Recovery_and_subsequent_characterization%29_1-s2.0-S1658365515001624-main.pdf |
| _version_ | 1825833610889920512 |
|---|---|
| author | Altaee, Nadia Fahdil, Ayad Yousif, Emad Sudesh, Kumar |
| author_facet | Altaee, Nadia Fahdil, Ayad Yousif, Emad Sudesh, Kumar |
| author_sort | Altaee, Nadia |
| collection | USM |
| description | The gram-positive bacterium Rhodococcus equi was isolated from fertile soil, and mineral salt media (MM) and trace elementswere used to provide the necessary elements for its growth and PHB production in addition to using crude palm kernel oil (CPKO) 1%as the carbon source. Gas chromatography (GC) demonstrated that the composition of the recovered biopolymer was homopolymerpolyhydroxybutyrate (PHB). The strain of the present study has a dry biomass of 1.43 (g/l) with 38% PHB, as determined by GC.The recovered PHB was characterized by NMR to study the chemical structure. In addition, DSC and TGA were used to study thethermal properties of the recovered polymer, where the melting temperature (Tm) was 173◦C, the glass transition temperature (Tg)was 2.79◦C, and the decomposition temperature (Td) was 276◦C. Gel permeation chromatography (GPC) was used to study themolecular mass of the recovered PHB in addition to comparing the results with other studies using different bacteria and substrates,where the molecular weight was 642 kDa, to enable its usage in many applications. The present study demonstrated the use of aninexpensive substrate for PHB production, i.e., using gram-positive bacteria to produce PHB polymer with characterization. |
| first_indexed | 2024-03-06T15:10:24Z |
| format | Article |
| id | usm.eprints-37183 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:10:24Z |
| publishDate | 2016 |
| publisher | Taibah University, Madinah, Kingdom of Saudi Arabia |
| record_format | dspace |
| spelling | usm.eprints-371832019-09-06T09:02:05Z http://eprints.usm.my/37183/ Recovery and subsequent characterization of polyhydroxybutyrate from Rhodococcus equi cells grown on crude palm kernel oil Altaee, Nadia Fahdil, Ayad Yousif, Emad Sudesh, Kumar QH1 Natural history (General - Including nature conservation, geographical distribution) The gram-positive bacterium Rhodococcus equi was isolated from fertile soil, and mineral salt media (MM) and trace elementswere used to provide the necessary elements for its growth and PHB production in addition to using crude palm kernel oil (CPKO) 1%as the carbon source. Gas chromatography (GC) demonstrated that the composition of the recovered biopolymer was homopolymerpolyhydroxybutyrate (PHB). The strain of the present study has a dry biomass of 1.43 (g/l) with 38% PHB, as determined by GC.The recovered PHB was characterized by NMR to study the chemical structure. In addition, DSC and TGA were used to study thethermal properties of the recovered polymer, where the melting temperature (Tm) was 173◦C, the glass transition temperature (Tg)was 2.79◦C, and the decomposition temperature (Td) was 276◦C. Gel permeation chromatography (GPC) was used to study themolecular mass of the recovered PHB in addition to comparing the results with other studies using different bacteria and substrates,where the molecular weight was 642 kDa, to enable its usage in many applications. The present study demonstrated the use of aninexpensive substrate for PHB production, i.e., using gram-positive bacteria to produce PHB polymer with characterization. Taibah University, Madinah, Kingdom of Saudi Arabia 2016-07 Article PeerReviewed application/pdf en http://eprints.usm.my/37183/1/%28Recovery_and_subsequent_characterization%29_1-s2.0-S1658365515001624-main.pdf Altaee, Nadia and Fahdil, Ayad and Yousif, Emad and Sudesh, Kumar (2016) Recovery and subsequent characterization of polyhydroxybutyrate from Rhodococcus equi cells grown on crude palm kernel oil. Journal of Taibah University for Science, 10 (4). pp. 543-550. ISSN 1658-3655 https://doi.org/10.1016/j.jtusci.2015.09.003 |
| spellingShingle | QH1 Natural history (General - Including nature conservation, geographical distribution) Altaee, Nadia Fahdil, Ayad Yousif, Emad Sudesh, Kumar Recovery and subsequent characterization of polyhydroxybutyrate from Rhodococcus equi cells grown on crude palm kernel oil |
| title | Recovery and subsequent characterization of polyhydroxybutyrate from Rhodococcus equi cells grown on crude palm kernel oil |
| title_full | Recovery and subsequent characterization of polyhydroxybutyrate from Rhodococcus equi cells grown on crude palm kernel oil |
| title_fullStr | Recovery and subsequent characterization of polyhydroxybutyrate from Rhodococcus equi cells grown on crude palm kernel oil |
| title_full_unstemmed | Recovery and subsequent characterization of polyhydroxybutyrate from Rhodococcus equi cells grown on crude palm kernel oil |
| title_short | Recovery and subsequent characterization of polyhydroxybutyrate from Rhodococcus equi cells grown on crude palm kernel oil |
| title_sort | recovery and subsequent characterization of polyhydroxybutyrate from rhodococcus equi cells grown on crude palm kernel oil |
| topic | QH1 Natural history (General - Including nature conservation, geographical distribution) |
| url | http://eprints.usm.my/37183/1/%28Recovery_and_subsequent_characterization%29_1-s2.0-S1658365515001624-main.pdf |
| work_keys_str_mv | AT altaeenadia recoveryandsubsequentcharacterizationofpolyhydroxybutyratefromrhodococcusequicellsgrownoncrudepalmkerneloil AT fahdilayad recoveryandsubsequentcharacterizationofpolyhydroxybutyratefromrhodococcusequicellsgrownoncrudepalmkerneloil AT yousifemad recoveryandsubsequentcharacterizationofpolyhydroxybutyratefromrhodococcusequicellsgrownoncrudepalmkerneloil AT sudeshkumar recoveryandsubsequentcharacterizationofpolyhydroxybutyratefromrhodococcusequicellsgrownoncrudepalmkerneloil |