Preliminary comparative analysis of antibacterial effects of activated and non-activated of expired platelet concentrate by disc diffusion method
Background: Platelets release more than 30 cytokines to provide primary hemostatic function. In addition, platelets are also known to release antimicrobial peptides upon activation by thrombin. Materials and Methods: In this study, comparative analysis of antibacterial activity of activated and non...
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| Format: | Article |
| Language: | English |
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Indian Association of Pathologists and Microbiologists
2012
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| Online Access: | http://eprints.usm.my/37573/1/Preliminary_comparative_analysis_of_antibacterial_effects-_azrul_zabidi.pdf |
| _version_ | 1797009861320376320 |
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| author | Zabidi, Muhammad Azrul Mohd Yusoff, Narazah Sheik Abdul Kader, Zainoodin |
| author_facet | Zabidi, Muhammad Azrul Mohd Yusoff, Narazah Sheik Abdul Kader, Zainoodin |
| author_sort | Zabidi, Muhammad Azrul |
| collection | USM |
| description | Background: Platelets release more than 30 cytokines to provide primary hemostatic function. In addition, platelets are also known to release antimicrobial peptides upon activation by
thrombin. Materials and Methods: In this study, comparative analysis of antibacterial activity of activated and non-activated expired platelet concentrate was determined against Grampositive
and Gram-negative bacteria by Kirby-Bauer disk diffusion method. Thrombin was used to prepare activated platelet concentrate. Gram-positive bacteria tested in this study were
S.aureus and S.pyogenes and Gram-negative bacteria were E.coli and K.oxytoca. All the bacteria used in this study were sensitive strains from clinical isolates. Activated and nonactivated
platelet showed no zone of inhibition against S.pyogenes and E.coli. Results: Activated platelet showed antibacterial activity against S.aureus and K.oxytoca with the zone of
inhibition of 8.3 ± 0.6 mm and 7.7 ± 0.2 mm, respectively. Zone of inhibition observed in non-activated platelet against S.aureus and K.oxytoca were 7.8 ± 0.4 mm and 7.5 ± 0.3 mm,
respectively. Conclusions: These findings showed that no significant differences in antibacterial activity produced by activated and non-activated platelet. However, zone of inhibition
observed in activated and non-activated platelet indicate the presence of antibacterial property in expired platelet. |
| first_indexed | 2024-03-06T15:11:31Z |
| format | Article |
| id | usm.eprints-37573 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:11:31Z |
| publishDate | 2012 |
| publisher | Indian Association of Pathologists and Microbiologists |
| record_format | dspace |
| spelling | usm.eprints-375732017-11-21T03:11:18Z http://eprints.usm.my/37573/ Preliminary comparative analysis of antibacterial effects of activated and non-activated of expired platelet concentrate by disc diffusion method Zabidi, Muhammad Azrul Mohd Yusoff, Narazah Sheik Abdul Kader, Zainoodin QR1-502 Microbiology Background: Platelets release more than 30 cytokines to provide primary hemostatic function. In addition, platelets are also known to release antimicrobial peptides upon activation by thrombin. Materials and Methods: In this study, comparative analysis of antibacterial activity of activated and non-activated expired platelet concentrate was determined against Grampositive and Gram-negative bacteria by Kirby-Bauer disk diffusion method. Thrombin was used to prepare activated platelet concentrate. Gram-positive bacteria tested in this study were S.aureus and S.pyogenes and Gram-negative bacteria were E.coli and K.oxytoca. All the bacteria used in this study were sensitive strains from clinical isolates. Activated and nonactivated platelet showed no zone of inhibition against S.pyogenes and E.coli. Results: Activated platelet showed antibacterial activity against S.aureus and K.oxytoca with the zone of inhibition of 8.3 ± 0.6 mm and 7.7 ± 0.2 mm, respectively. Zone of inhibition observed in non-activated platelet against S.aureus and K.oxytoca were 7.8 ± 0.4 mm and 7.5 ± 0.3 mm, respectively. Conclusions: These findings showed that no significant differences in antibacterial activity produced by activated and non-activated platelet. However, zone of inhibition observed in activated and non-activated platelet indicate the presence of antibacterial property in expired platelet. Indian Association of Pathologists and Microbiologists 2012 Article PeerReviewed application/pdf en cc_by http://eprints.usm.my/37573/1/Preliminary_comparative_analysis_of_antibacterial_effects-_azrul_zabidi.pdf Zabidi, Muhammad Azrul and Mohd Yusoff, Narazah and Sheik Abdul Kader, Zainoodin (2012) Preliminary comparative analysis of antibacterial effects of activated and non-activated of expired platelet concentrate by disc diffusion method. Indian Journal of Pathology and Microbiology, 55 (1). pp. 47-51. ISSN 0377-4929 https://doi.org/10.4103/0377-4929.94855 |
| spellingShingle | QR1-502 Microbiology Zabidi, Muhammad Azrul Mohd Yusoff, Narazah Sheik Abdul Kader, Zainoodin Preliminary comparative analysis of antibacterial effects of activated and non-activated of expired platelet concentrate by disc diffusion method |
| title | Preliminary comparative analysis of antibacterial effects of activated and non-activated of expired platelet concentrate by
disc diffusion method |
| title_full | Preliminary comparative analysis of antibacterial effects of activated and non-activated of expired platelet concentrate by
disc diffusion method |
| title_fullStr | Preliminary comparative analysis of antibacterial effects of activated and non-activated of expired platelet concentrate by
disc diffusion method |
| title_full_unstemmed | Preliminary comparative analysis of antibacterial effects of activated and non-activated of expired platelet concentrate by
disc diffusion method |
| title_short | Preliminary comparative analysis of antibacterial effects of activated and non-activated of expired platelet concentrate by
disc diffusion method |
| title_sort | preliminary comparative analysis of antibacterial effects of activated and non activated of expired platelet concentrate by disc diffusion method |
| topic | QR1-502 Microbiology |
| url | http://eprints.usm.my/37573/1/Preliminary_comparative_analysis_of_antibacterial_effects-_azrul_zabidi.pdf |
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